...Manolo U. Rios; Weronika E. Stachera; Nicole E. Familiari; Claudia Brito; Thomas Surrey; Jeffrey B. Woodruff ABSTRACT CDK5RAP2 (also known as CEP215) is a key pericentriolar material (PCM) protein that recruits microtubule-nucleating factors at human centrosomes. Here, using an in vitro...

... complex, inter-organelle communication and coordination of cellular components. In this study, we report a role of γ-tubulin in DSB repair. γ-tubulin is a major microtubule nucleation factor governing microtubule dynamics. We show that γ-tubulin is recruited to the site of DNA damage and is required...

...Shingo Koinuma; Misa Miyaji; Suzuka Akiyama; Yasuyuki Ito; Hiroshi Takemura; Naoyuki Wada; Michihiro Igarashi; Takeshi Nakamura ABSTRACT The neuronal cytoskeleton comprises microtubules, actin filaments and neurofilaments, and plays a crucial role in axon outgrowth and transport. Microtubules...

...Emma J. van Grinsven; Anna Akhmanova ABSTRACT Microtubules are cytoskeletal filaments important for various cellular processes such as intracellular transport, cell division, polarization and migration. Microtubule organization goes hand in hand with cellular function. Motile cells, such as immune...

... forces. Our previous findings demonstrate that VSMC volume is enhanced in response to increased matrix rigidity, but our understanding of the mechanisms regulating this process remain incomplete. In this study, we show that microtubule stability in VSMCs is reduced in response to enhanced matrix rigidity...

...Luisa F. Arias Padilla; Jonathan Munera Lopez; Aika Shibata; John M. Murray; Ke Hu ABSTRACT The body plan of the human parasite Toxoplasma gondii has a well-defined polarity. The minus ends of the 22 cortical microtubules are anchored to the apical polar ring, which is a putative microtubule...

...Xiaohuan Sun; Wenqian Yu; Peter W. Baas; Kazuhito Toyooka; Liang Qiang ABSTRACT Association of tau (encoded by Mapt) with microtubules causes them to be labile, whereas association of MAP6 with microtubules causes them to be stable. As axons differentiate and grow, tau and MAP6 segregate from one...

... cilia of murine intramedullary collecting duct cells. The shortening of primary cilia caused by hyperosmotic shock followed delocalization of the pericentriolar material (PCM). Excessive microtubule and F-actin formation in the cytoplasm coincided with the hyperosmotic shock-induced changes to primary...

...Matthieu Gélin; Alexandre Schaeffer; Jérémie Gaillard; Christophe Guérin; Benoit Vianay; Magali Orhant-Prioux; Marcus Braun; Christophe Leterrier; Laurent Blanchoin; Manuel Théry ABSTRACT The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates...

...Nami Haruta; Eisuke Sumiyoshi; Yu Honda; Masahiro Terasawa; Chihiro Uchiyama; Mika Toya; Yukihiko Kubota; Asako Sugimoto ABSTRACT The γ-tubulin complex (γTuC) is a widely conserved microtubule nucleator, but some of its components, namely GCP4, GCP5 and GCP6 (also known as TUBGCP4, TUBGCP5...

...Kristen J. Verhey; Ryoma Ohi ABSTRACT Microtubules are critical for a variety of important functions in eukaryotic cells. During intracellular trafficking, molecular motor proteins of the kinesin superfamily drive the transport of cellular cargoes by stepping processively along the microtubule...

...-regulating sequences, correlate with colorectal cancer. The cellular roles of APC in mitosis are widely studied, but the molecular mechanisms of its interaction with the cytoskeleton are poorly understood. Here, we investigated how APC-C regulates microtubule properties, and found that it promotes both...

...Olivera Mitevska; Pak Wing Lam; Lydia Daly; Philip Auckland ABSTRACT Mitotic cell division requires that kinetochores form microtubule attachments that can segregate chromosomes and control mitotic progression via the spindle assembly checkpoint. During prometaphase, kinetochores shed a domain...

... unknown. Here, we investigate the role of actin, Rho GTPases and microtubules, which are major players in CIE processes, in this mechanism. We show that the actin cytoskeleton is dynamically associated with endoA3- and CD166-positive endocytic carriers, and that its perturbation strongly inhibits...

...Toni McHugh; Julie P. I. Welburn ABSTRACT The precise regulation of microtubule length during mitosis is essential to assemble and position the mitotic spindle and segregate chromosomes. The kinesin-13 Kif2C or MCAK acts as a potent microtubule depolymerase that diffuses short distances...

...Emmanuel T. Nsamba; Mohan L. Gupta, Jr ABSTRACT The microtubule cytoskeleton is assembled from the α- and β-tubulin subunits of the canonical tubulin heterodimer, which polymerizes into microtubules, and a small number of other family members, such as γ-tubulin, with specialized functions. Overall...

...Joseph Atherton; Melissa Stouffer; Fiona Francis; Carolyn A. Moores ABSTRACT Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone...

...Ali Nick Maleki; Pim J. Huis in 't Veld; Anna Akhmanova; Marileen Dogterom; Vladimir A. Volkov ABSTRACT Microtubules are dynamic cytoskeletal filaments that can generate forces when polymerizing and depolymerizing. Proteins that follow growing or shortening microtubule ends and couple forces...

... ABSTRACT Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary...

...Akanksha Thawani; Sabine Petry ABSTRACT As one of four filament types, microtubules are a core component of the cytoskeleton and are essential for cell function. Yet how microtubules are nucleated from their building blocks, the αβ-tubulin heterodimer, has remained a fundamental open question since...