...Edismauro G. Freitas Filho; Elaine Z. M. da Silva; Hwei Ling Ong; William D. Swaim; Indu S. Ambudkar; Constance Oliver; Maria Célia Jamur ABSTRACT Although RACK1 is known to act as a signaling hub in immune cells, its presence and role in mast cells (MCs) is undetermined. MC activation via antigen...

...Hye Sun Kuehn; Madeleine Rådinger; Jared M. Brown; Khaled Ali; Bart Vanhaesebroeck; Michael A. Beaven; Dean D. Metcalfe; Alasdair M. Gilfillan Mast cells infiltrate the sites of inflammation associated with chronic atopic disease and during helminth and bacterial infection. This process requires...

...Frida Henningsson; Kenji Yamamoto; Paul Saftig; Thomas Reinheckel; Christoph Peters; Stefan D. Knight; Gunnar Pejler Mast-cell carboxypeptidase A is stored in the secretory granule and is released, together with a range of other inflammatory mediators, upon mast-cell degranulation. Carboxypeptidase...

...; by contrast,biologically inactive C 2 -dihydroceramide does neither. Structural perturbations by these ceramides parallel their inhibitory effects on antigen-stimulated Ca 2+ mobilization in RBL mast cells in the presence and absence of extracellular Ca 2+ . Similar inhibition of Ca 2+ mobilization is caused...

...), a member of the Syt family of proteins, is required for TPA-induced degradation of PKCα. Thus, whereas the kinase half-life in TPA-treated cultured mast cells (the mast cell line rat basophilic leukemia RBL-2H3) is 2 hours, it is doubled in RBL-Syt II - cells, in which the cellular level of Syt II...

...Keli Xu; Rebecca M. Williams; David Holowka; Barbara Baird ABSTRACT Sensitization of RBL-2H3 mast cells with monomeric fluorescein-5-isothiocyanate (FITC)-labeled immunoglobulin E (IgE) results in slow but highly efficient accumulation of labeled IgE fragments in a pool of acidic peripheral...

... of membrane permeabilisation affect the requirements for Ca 2+ and guanine nucleotide, we have introduced such effectors directly into intact, rat peritoneal mast cells by microinjection. During this brief procedure (∼1 s) a glass needle forms a seal with the plasma membrane. Following injection...

...A. Koffer; B. D. Gomperts ABSTRACT This study addresses the question of the role of cytoplasmic proteins in exocytosis from permeabilised rat mast cells. We have used two different methods of cell permeabilisation (ATP 4− and streptolysin O) to regulate the size of the plasma membrane lesions...

...I. Hammel; H. Shiloh-Rabinovich; I. Nir ABSTRACT We have previously shown that a confluent layer of mature mast cells is obtained when lymph node cells are grown on embryonic flbroblast monolayers. Two populations of mast cells may be observed, depending on treatment of the mice from which...