... facilitate Leishmania entry into macrophages and pathogenesis in murine cutaneous leishmaniasis. Here, we demonstrate that host spleen tyrosine kinase (SYK) is required for efficient uptake of Leishmania promastigotes and amastigotes. A Src-family kinase–Abl-family kinase–SYK signaling cascade induces...

...Priyanka Ghosh; Kyo Sasaki; Isabel Aranzazu Pulido Ruiz; Kayla E. King; Steven A. Weinman; Ann L. Wozniak ABSTRACT Macrophage-derived extracellular vesicles (EVs) play key roles in intercellular communication. Within the liver, they have been linked to several inflammatory diseases including...

...Vasco Rodrigues; Sarah Taheraly; Mathieu Maurin; Mabel San-Roman; Emma Granier; Anaël Hanouna; Philippe Benaroch ABSTRACT A feature of HIV-1 replication in macrophages is that viral assembly occurs at the limiting membrane of a compartment often named the virus-containing compartment (VCC...

...Kuihao Chen; Qiaoyan Man; Jiaen Miao; Wenjing Xu; Yangchen Zheng; Xiuli Zhou; Zhe Gao ABSTRACT Macrophage polarization plays a key role in the inflammatory response. Various ion channels expressed in macrophages have been documented, but very little is known about their roles in macrophage...

...Aarthi Subramani; Maria E. L. Hite; Sarah Garcia; Jack Maxwell; Hursha Kondee; Grace E. Millican; Erin E. McClelland; Rebecca L. Seipelt-Thiemann; David E. Nelson ABSTRACT Macrophages serve as a first line of defense against microbial pathogens. Exposure to interferon-γ (IFNγ) increases interferon...

...Marielle Saclier; Sabrina Ben Larbi; Ha My Ly; Eugénie Moulin; Rémi Mounier; Bénédicte Chazaud; Gaëtan Juban ABSTRACT Duchenne muscular dystrophy is a genetic muscle disease characterized by chronic inflammation and fibrosis mediated by a pro-fibrotic macrophage population expressing pro...

... of borreliae by immune cells, like macrophages, is decisive for the outcome of a respective infection. Here, we use, for the first time, focused ion beam scanning electron microscopy tomography (FIB-SEM tomography) to visualize the interaction of borreliae with primary human macrophages with high resolution...

... RAW264.7 macrophages and primary murine macrophages undergoing interleukin (IL-4)-induced MGC formation. CD301 + MGCs were identified in biopsy specimens of human inflammatory lesions. Function-inhibiting CD301 antibodies or CRISPR/Cas9 deletion of the two mouse CD301 genes (Mgl1 and Mgl2) inhibited IL-4...

... kinase BTK Macrophage Receptor signaling Else-Kröner-Fresenius Stiftung 2013_A210 Deutsche Forschungsgemeinschaft 10.13039/501100001659 CRC TR156 We-4195/15-1 Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg 10.13039/501100003542 33-7533-7...

... of xenophagy in macrophages targets Mtb to autophagosomes; however, how Mtb is targeted to autophagosomes in human macrophages at a high spatial and temporal resolution is unknown. Here, we use human induced pluripotent stem cell-derived macrophages (iPSDMs) to study the human macrophage response to Mtb...

...Madhavi Vijayan; Cheuk-Lun Lee; Vera H. H. Wong; Xia Wang; Kungfeng Bai; Jian Wu; Hannu Koistinen; Markku Seppälä; Kai-Fai Lee; William S. B. Yeung; Ernest H. Y. Ng; Philip C. N. Chiu ABSTRACT Decidual macrophages constitute 20–30% of the total leukocytes in the uterus of pregnant women, regulating...

... responses in the non-ciliated macrophage. We propose that ciliary proteins, including IFT88, KIF3A, TTBK2 and NPHP4, act outside of the ciliary axoneme to tune cytoplasmic NFκB signalling and specify the downstream cell response. This is thus a non-canonical function for ciliary proteins in shaping cellular...

...Thibault Rosazza; Hervé Lecoeur; Thierry Blisnick; Maryse Moya-Nilges; Pascale Pescher; Phillipe Bastin; Eric Prina; Gerald F. Späth ABSTRACT Leishmania spp. are obligate intracellular parasites that infect phagocytes, notably macrophages. No information is available on how Leishmania parasites...

...:   Leishmania effluxes host ATP generated from increased glycolysis, which is converted into extracellular adenosine to create an anti-inflammatory milieu within infected cells via receptor-mediated signalling. ATP Ectonucleotidase Adenosine receptor Leishmania donovani Macrophage...

... of phagocytosis, and changes in macrophage morphology during phagocytosis related to both engulfment and target degradation. Our results provide a high-resolution method for quantifying phagocytosis that provides opportunities to better understand the cellular and molecular regulation of this fundamental...

...Rajesh K. Singh; Frederik W. Lund; Abigail S. Haka; Frederick R. Maxfield ABSTRACT Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process...

... rearrangements drive macrophage recruitment to wounds in Drosophila . We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages, where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin...

... to form PtdIns(5)P in vitro . However, the in vivo conversion of the polyphosphoinositide into PtdIns(5)P by the phosphatase has not yet been demonstrated, and the role of TMEM55a remains poorly understood. Here, we found that mouse macrophages (Raw264.7) deficient in TMEM55a showed an increased...

..., interferes with tip and stalk cell specification, and hinders the function of filopodial protrusions required for precise guidance of vascular sprouts. In addition, ABA skews macrophage polarization towards the M1 phenotype characterized by anti-angiogenic marker expression. In accordance with this, ABA...

...Francisco Barros-Becker; Pui-Ying Lam; Robert Fisher; Anna Huttenlocher ABSTRACT Cell motility is required for diverse processes during immunity and inflammation. Classically, leukocyte motility is defined as an amoeboid type of migration, however some leukocytes, like macrophages, also employ...