..., which enhances its interaction with Gle1. Furthermore, a SUMOylation-defective mutant of human DDX19B, K26R, failed to provide a complete rescue of the mRNA export defect caused by DDX19 depletion. Collectively, our results suggest that SUMOylation fine-tunes the function of DDX19 in mRNA export...

...David Umlauf; Jacques Bonnet; François Waharte; Marjorie Fournier; Matthieu Stierle; Benoit Fischer; Laurent Brino; Didier Devys; László Tora Summary In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC...

..., optimally preserved by low-temperature fixation and then localized by immunoelectron microscopy. A statistical modelling approach was used to identify cargo distribution. We found import cargos localized surprisingly close to the edge of the channel, whereas mRNA export factors were at the very centre...

... and the diverse emerging roles of the NPC environment in promoting gene expression, focusing on yeast as a model organism. * Author for correspondence ( [email protected] ) © 2010. 2010 Nuclear pore complex NPC Transcription mRNA export mRNA surveillance Yeast The positioning...

...-mail: [email protected] ) 21 5 2007 © The Company of Biologists Limited 2007 2007 mRNA export Noncoding RNA Nuclear domains Nuclear matrix The classical view that RNA molecules are dedicated solely to protein synthesis has been rapidly changing ( Mattick, 2003 ; Andersen...

...Reiko Takemura; Yoshiharu Inoue; Shingo Izawa Ethanol stress (10% v/v) causes selective mRNA export in Saccharomyces cerevisiae in a similar manner to heat shock (42°C). Bulk poly(A) + mRNA accumulates in the nucleus, whereas heat shock protein mRNA is exported under such conditions. Here we...

...Takashi Ideue; Abul Kalam Azad; Jun-ichi Yoshida; Tadao Matsusaka; Mitsuhiro Yanagida; Yasumi Ohshima; Tokio Tani To elucidate the mechanism of mRNA export from the nucleus, we isolated five novel temperature-sensitive mutants ( ptr7 to ptr11 ) that accumulate poly(A) + RNA in the nuclei...

... Institute, SE-171 67 Stockholm, Sweden 17 11 2003 © The Company of Biologists Limited 2004 2004 Gene expression mRNA export Export receptors Transport of RNA and proteins to and from the cell nucleus occurs through the nuclear pore complex (NPC) ( Stutz and Rosbach, 1998...

... and microinjected mRNAs. Using such a procedure, we were able to detect a direct interaction between nucleoporin p62 with mRNA during export. The formation of the mRNA-p62 complex was inhibited by wheat-germ agglutinin, an inhibitor of mRNA export. Antibodies directed against p62 caused a substantial reduction...

... Institute for Biochemical Regulation, Chikusa Nagoya 464-01, Japan 03 05 1994 06 09 1994 © 1995 by Company of Biologists 1995 yeast nucleus mRNA export protein import Following transcription, mRNA is processed at both ends and any non-coding intervening sequences may...