...Ana C. Almeida; Helder Rocha; Maximilian W. D. Raas; Hanh Witte; Ralf J. Sommer; Berend Snel; Geert J. P. L. Kops; Reto Gassmann; Helder Maiato ABSTRACT Chromosome alignment during mitosis can occur as a consequence of bi-orientation or is assisted by the CENP-E (kinesin-7) motor at kinetochores...

...Laura Bel Borja; Samuel J. P. Taylor; Flavie Soubigou; Federico Pelisch ABSTRACT Polo-like kinase 1 (PLK-1) is present in centrosomes, the nuclear envelope and kinetochores and plays a significant role in meiosis and mitosis. PLK-1 depletion or inhibition has severe consequences for spindle...

..., although the specific mechanisms are not fully understood. Here, we investigated how CSR-1 regulates centromere and kinetochore function in C. elegans embryos. We found that depletion of CSR-1 results in defects in mitotic progression and chromosome positioning relative to the spindle pole. Knockdown...

... cycle arrest. To elucidate their contributions to chromosome segregation, in this work, we investigated their centromere- and kinetochore-related functions of Bmh1 and Bmh2. Analysis of appropriate deletion mutants shows that Bmh isoforms have cumulative and non-shared isoform-specific contributions...

...JingHui Cao; Tetsuya Hori; Mariko Ariyoshi; Tatsuo Fukagawa ABSTRACT The kinetochore is an essential structure for chromosome segregation. Although the kinetochore is usually formed on a centromere locus, it can be artificially formed at a non-centromere locus by protein tethering. An artificial...

... with that of other microtubule-associated proteins (MAPs) present on the same cargo. Here, these questions will be discussed in the context of dynein at the kinetochore, the supramolecular protein structure that connects segregating chromosomes to spindle microtubules in dividing cells. As the first kinetochore...

...Yanze Jian; Lingyun Nie; Sikai Liu; Yueyue Jiang; Zhen Dou; Xing Liu; Xuebiao Yao; Chuanhai Fu ABSTRACT The outer kinetochore serves as a platform for the initiation of the spindle assembly checkpoint (SAC) and for mediating kinetochore–microtubule attachments. How the inner kinetochore subcomplex...

...Olivera Mitevska; Pak Wing Lam; Lydia Daly; Philip Auckland ABSTRACT Mitotic cell division requires that kinetochores form microtubule attachments that can segregate chromosomes and control mitotic progression via the spindle assembly checkpoint. During prometaphase, kinetochores shed a domain...

... Summary: A DNA molecule folded in a shape of a spring is used to measure forces generated by dynamic microtubule ends, and to record single-molecule fluorescence at the site of force generation. DNA origami Force sensor Microtubule Dynein Kinetochore Optical trap European Molecular...

...Josh Lawrimore; Kerry Bloom ABSTRACT The centromere serves as the binding site for the kinetochore and is essential for the faithful segregation of chromosomes throughout cell division. The point centromere in yeast is encoded by a ∼115 bp specific DNA sequence, whereas regional centromeres range...

...Masashi Nambu; Atsuki Kishikawa; Takatomi Yamada; Kento Ichikawa; Yunosuke Kira; Yuta Itabashi; Akira Honda; Kohei Yamada; Hiroshi Murakami; Ayumu Yamamoto ABSTRACT Kinetochores drive chromosome segregation by mediating chromosome interactions with the spindle. In higher eukaryotes, sister...

... to the centromeres depends on BUBR1, MEI-S332 and kinetochore protein SPC105R. Although BUBR1 stabilizes microtubule attachments in Drosophila oocytes, it is not required for cohesion maintenance during meiosis I. We propose at least three populations of PP2A-B56 regulate meiosis, two of which depend on SPC105R...

... amphitelic attachments to microtubules emanating from opposing spindle poles. The astrin–kinastrin (astrin is also known as SPAG5 and kinastrin as SKAP) complex, also containing DYNLL1 and MYCBP, is a spindle and kinetochore protein complex with important roles in bipolar spindle formation, chromosome...

... show that this protocol could be successfully employed for the characterization of the protein–protein interaction network of the kinetochore protein Ndc80 by immunoprecipitation coupled with mass spectrometry. This synchronization method significantly expands the versatility and applicability of RPE-1...

... to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but the extent to which BubR1 contributes to CENP-E localization at kinetochores has remained controversial. Here we define...

... to stabilize centromere protein levels and accurate mitotic segregation. Pericentric heterochromatin levels can also recover after severe depletion. Centromere Heterochromatin Polycomb Mitosis Kinetochore CENP-A Human artificial chromosome Epigenetic engineering Wellcome Trust 10.13039...

... replication and chromosome segregation. The parasite undergoes atypical endomitosis and endoreduplication with an intact nuclear membrane and intranuclear mitotic spindle. To understand these diverse modes of Plasmodium cell division, we have studied the behaviour and composition of the outer kinetochore...

...Hiral Shah; Kanika Rawat; Harsh Ashar; Rajesh Patkar; Johannes Manjrekar ABSTRACT The outer kinetochore DASH complex (also known as the Dam1 complex) ensures proper spindle structure and chromosome segregation. While DASH complex protein requirement diverges among different yeasts, its role...

...Banafsheh Etemad; Abel Vertesy; Timo E. F. Kuijt; Carlos Sacristan; Alexander van Oudenaarden; Geert J. P. L. Kops ABSTRACT The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by monitoring kinetochore–microtubule interactions. SAC proteins are shed from kinetochores once...

...Julie K. Monda; Iain M. Cheeseman ABSTRACT Accurate chromosome segregation critically depends on the formation of attachments between microtubule polymers and each sister chromatid. The kinetochore is the macromolecular complex that assembles at the centromere of each chromosome during mitosis...