..., in the pathogenesis of hereditary human colon cancer. To date, however, the contributions of MCC to intestinal homeostasis and disease remain unclear. Here, we examine the subcellular localization of MCC, both at the mRNA and protein levels, in the adult intestinal epithelium. Our findings reveal that Mcc transcripts...

...Thaher Pelaseyed; Gunnar C. Hansson; Ana-Maria Lennon-Duménil ABSTRACT Membrane mucins cover most mucosal surfaces throughout the human body. The intestine harbors complex population of microorganisms (the microbiota) and numerous exogenous molecules that can harm the epithelium. In the colon...

... modification of heparan sulfate is crucial for regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular heparan sulfate endosulfatase Sulf1 resulted in increased ISC division during normal homeostasis, which was caused...

...Yannick D. Benoit; Manon B. Lepage; Taoufik Khalfaoui; Éric Tremblay; Nuria Basora; Julie C. Carrier; Lorraine J. Gudas; Jean-François Beaulieu Summary The crypt–villus axis constitutes the functional unit of the small intestine, where mature absorptive cells are confined to the villi, and stem...

... small intestine. Here, we demonstrate that PTK6 associates with nuclear and cytoplasmic β-catenin and inhibits β-catenin- and T-cell factor (TCF)-mediated transcription. PTK6 directly phosphorylates β-catenin on Tyr64, Tyr142, Tyr331 and/or Tyr333, with the predominant site being Tyr64. However...

...) of intestinal epithelial cells and might have an important impact on intestinal tumorigenesis. We demonstrate here that the expression of PKCα in proliferating intestinal epithelial cells is repressed both in vitro and in vivo by the SOX9 transcription factor. This repression does not require DNA binding...

...). Perhaps, because they are relatively simple organisms, worms can change their lipid composition without compromising the survival or function of most of their cells. It is not clear what essential functions the GSLs serve in the intestinal cells in C. elegans , where normal GSL levels are required...

.... Ophthalmol. Vis. Sci. 44 , 4097 -4104. Bertelsen, L. S., Eckmann, L. and Barrett, K. E. ( 2004 ). Prolonged interferon-gamma exposure decreases ion transport, NKCC1, and Na+-K+-ATPase expression in human intestinal xenografts in vivo. Am. J. Physiol. 286 , G157 -G165. Bijlsma, P. B., Kiliaan...

... of neuronal cells (NG108-15) with subepithelial fibroblasts, we confirmed that mechanically induced Ca 2+ -waves propagated to neurons. From these findings we propose that subepithelial fibroblasts work as a mechanosensor in the intestine. Uptake of food, water and nutrients may cause mechanical stress...

... of which was prevented by follistatin, was typical of an intestinal wall. Consistent with this interpretation, activin A rapidly induced in the explants the mRNAs for fatty acid binding proteins (FABPs), which are markers of the intestine, but not of the pancreas. We also found that induction of the FABPs...

... in the inflamed mucosa of patients with ulcerative colitis. Inflamm. Bowel Dis. 7 , 210 -214. Beaulieu, J. F., Nichols, B. and Quaroni, A. ( 1989 ). Posttranslational regulation of sucrase-isomaltase expression in intestinal crypt and villus cells. J. Biol. Chem. 264 , 20000 -20011. Bender, F. C...

...Christopher S. Potten; Gary Owen; Dawn Booth The stem cells in the crypts of the small intestinal mucosa divide about a thousand times during the lifespan of a laboratory mouse, and yet they show little evidence of any decline in proliferative potential and rarely develop carcinogenic mutations...

...Wenle Wang; Sergio Uzzau; Simeon E. Goldblum; Alessio Fasano ABSTRACT Intercellular tight junctions are dynamic structures involved in vectorial transport of water and electrolytes across the intestinal epithelium. Zonula occludens toxin derived from Vibrio cholerae interacts with a specific...

... adulthood and to be predominantly localized at the periphery of a population of large, membrane-bound organelles, called granules, that are seen throughout the early embryo but in later stages are restricted to the cells of the intestine. Analysis of an LMP-1 deficient C. elegans mutant revealed that LMP-1...

... and milk by the small intestine of the suckling newborn. In both cases apical to basolateral transepithelial transport of IgG is thought to be mediated by FcRn, an IgG Fc receptor with homology to MHC class I antigens. We have now expressed the human FcRn in polarized MDCK cells and analyzed...

... set of antibody reagents. Immunoblot analysis demonstrated that adseverin was highly expressed in mouse kidney and intestine at all stages of development and in human fetal and adult kidney. In contrast and as reported previously, gelsolin was expressed much more widely in both murine and human...

... of the regulation of laminin-5 in normal and pathological intestine. REFERENCES Aberdam , D. , Galliano , M. F. , Mattei , M. G. , Pisani-Spadafora , A. , Ortonne , J. P. and Meneguzzi , G. ( 1994a ). Assignment of mouse nicein genes to chromosomes 1 and 18 . Mamm. Genome 5...

...Catharina E. C. Moolenaar; Joke Ouwendijk; Michael Wittpoth; Heleen A. Wisselaar; Hans-Peter Hauri; Leo A. Ginsel; Hassan Y. Naim; Jack A. M. Fransen ABSTRACT A point mutation in the cDNA of human intestinal sucrase-isomaltase has been recently identified in phenotype II of congenital sucrase...

...Roger A. Brooks; Douglas J. Winton ABSTRACT We have developed a method to quantitate DNA strand breaks as a measure of DNA damage and repair in intact, isolated intestinal crypts. The assay is a modified form of the single-cell gel electrophoresis or ‘comet’ assay. By maintaining the spatial...

...S. Heusser; S. Colin; A. Figiel; C. Huet; J.M. Keller; P. Pornet; S. Robine; J. Vandamme; J. Vandekerckhove; M. DAUçA ABSTRACT An actin-binding protein of M r 105,000 has been isolated from anuran amphibian intestinal mucosa. Polyclonal antibodies directed against chicken and pig intestinal villins...