... of proteins. Apical integrins have been identified as potential regulators of epithelial barrier function, however, only indirect approaches have been used to measure integrin regulation of the epithelial barrier. Here, we used polymeric nanowires conjugated with anti-integrin β1 antibodies to specifically...

... angiogenesis. YAP/TAZ Mechanotransduction Focal adhesion DLC1 Rho GTPase Force Endothelium Migration Angiogenesis Integrin Stiffness Nederlandse Organisatie voor Wetenschappelijk Onderzoek http://dx.doi.org/10.13039/501100003246 VIDI 016.156.327 Hartstichting http...

...Georgina P. Coló; Andrea Seiwert; Raquel B. Haga ABSTRACT Fibronectin (FN)-binding integrins control a variety of cellular responses through Rho GTPases. The FN-binding integrins, αvβ3 and α5β1, are known to induce different effects on cell morphology and motility. Here, we report that FN-bound...

...Julien Pernier; Marcelina Cardoso Dos Santos; Mariem Souissi; Adrien Joly; Hemalatha Narassimprakash; Olivier Rossier; Grégory Giannone; Emmanuèle Helfer; Kheya Sengupta; Christophe Le Clainche ABSTRACT Focal adhesions are composed of transmembrane integrins, linking the extracellular matrix...

... phosphorylation by mass spectrometry and in vitro kinase assay. Focusing on S131 and S146, we demonstrate that they have a role in SHARPIN-ARP2/3 complex interaction, but play no role in integrin inhibition or LUBAC activation. Consistent with its novel role in ARP2/3 regulation, S146 phosphorylation of SHARPIN...

...Xuekai Xiong; Weini Li; Jin Nam; Meng Qu; Steve A. Kay; Ke Ma ABSTRACT The circadian clock is entrained to daily environmental cues. Integrin-linked signaling via actin cytoskeleton dynamics transduces physical niche cues from the extracellular matrix to myocardin-related transcription factor (MRTF...

...Alba Zuidema; Wei Wang; Maaike Kreft; Onno B. Bleijerveld; Liesbeth Hoekman; Jonas Aretz; Ralph T. Böttcher; Reinhard Fässler; Arnoud Sonnenberg ABSTRACT The vitronectin receptor integrin αVβ5 can reside in two distinct adhesion structures – focal adhesions (FAs) and flat clathrin lattices (FCLs...

... laterally (dark orange) and is less concentrated medially (light orange). (E) Formation of this dynamic gradient of Fibronectin matrix depends upon shear stress at the interface of the neural tube and PSM, as well as activated Integrin α5β1. In the development of feather bud primordia, inter...

... at the leading and lagging edge of migrating cells. We find that cells lacking ALFY have reduced attachment and altered protein levels and glycosylation of integrins, resulting in the inability to form a proper leading edge and loss of directional cell motility. * These authors contributed equally...

...Sarah Klapproth; Karsten Richter; Clara Türk; Theresa Bock; Thomas Bromberger; Julian Dominik; Kathrin Huck; Kristian Pfaller; Michael W. Hess; Christoph A. Reichel; Marcus Krüger; Inaam A. Nakchbandi; Markus Moser ABSTRACT Osteoclasts form special integrin-mediated adhesion structures called...

...Benjamin T. Goult; Nicholas H. Brown; Martin A. Schwartz ABSTRACT Talins are cytoskeletal linker proteins that consist of an N-terminal head domain, a flexible neck region and a C-terminal rod domain made of 13 helical bundles. The head domain binds integrin β-subunit cytoplasmic tails, which...

... to the underlying basement membrane. Integrin α6β4 is a receptor for laminins and plays a vital role in mediating cell adhesion by initiating the assembly of HDs. In addition, α6β4 has been implicated in signal transduction events that regulate diverse cellular processes, including proliferation and survival...

...Keith P. Eidell; Alenka Lovy; Nicholas R. Sylvain; Frank A. Scangarello; Hayley I. Muendlein; Michael J. Ophir; Ken Nguyen; Maria-Cristina Seminario; Stephen C. Bunnell ABSTRACT Integrin engagement within the immune synapse enhances T cell activation, but our understanding of this process...

...Wenting Bu; Zarina Levitskaya; Suet-Mien Tan; Yong-Gui Gao ABSTRACT Integrin-mediated cell–extracellular matrix (ECM) interactions play crucial roles in a broad range of physiological and pathological processes. Kindlins are important positive regulators of integrin activation. The FERM-domain...

...Farhana A. Sarker; Victoria G. Prior; Samuel Bax; Geraldine M. O'Neill ABSTRACT Research throughout the 90s established that integrin crosstalk with growth factor receptors stimulates robust growth factor signaling. These insights were derived chiefly from comparing adherent versus suspension cell...

... by mechanical forces transmitted across cell surface β1 integrin receptors on endothelial cells, and the transmembrane solute carrier family 3 member 2 (herein denoted CD98hc, also known as SLC3A2) protein has been implicated in this response. Here, we show that β1 integrin, CD98hc and TRPV4 all tightly...

...; Jinhua Wu; Vesa P. Hytönen; Bernhard Wehrle-Haller ABSTRACT Integrin activation and clustering by talin are early steps of cell adhesion. Membrane-bound talin head domain and kindlin bind to the β integrin cytoplasmic tail, cooperating to activate the heterodimeric integrin, and the talin head domain...

... the mechanics of adhesion, we employed tension gauge tether (TGT) probes displaying the integrin ligand cRGDfK and quantified integrin tension. EGF exposure significantly increased spread area, cell circularity, integrated integrin tension, mechanical rupture density, radial organization and size of focal...

...Hao Xu; Kevin Pumiglia; Susan E. LaFlamme ABSTRACT During angiogenesis, endothelial cells engage components of the extracellular matrix through integrin-mediated adhesion. Endothelial expression of laminin-411 and laminin-511 is known to promote vessel stability. However, little is known about...

... to sites of ECM remodeling. Internalization of MT1-MMP is dependent on its phosphorylation, and here we examine the role of β1 integrin-mediated signaling in this process. Activation of β1 integrin using the antibody P4G11 induced phosphorylation and internalization of MT1-MMP and resulted in increased...