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Keywords: in situ hybridization
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Journal Articles
J Cell Sci (2020) 133 (7): jcs239756.
Published: 8 April 2020
... of mRNA ex vivo in complex brain cells, such as astrocytes and microglia, in physiological and pathological conditions. Astrocytes Microglia Hippocampus mRNA In situ hybridization Immunofluorescence ImageJ APPswe/PS1dE9 mouse Alzheimer's disease GFAP Astrocytes are the most...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (6): 1299–1307.
Published: 15 March 2005
... into the oocyte nucleus became specifically localized in the nucleoli, and endogenous SRP RNA was also detected in oocyte nucleoli by RNA in situ hybridization. An initial step in the assembly of SRP involves the binding of the SRP19 protein to SRP RNA. When green fluorescent protein (GFP)-tagged SRP19 protein...
Journal Articles
J Cell Sci (2003) 116 (21): 4467–4478.
Published: 1 November 2003
.... In situ hybridization showed that poly(A) mRNA is abundant and non-uniformly distributed in nearly all axons. The specific transcripts for β-actin and actin-depolymerizing factor (ADF) were also present and non-uniformly distributed in axons, with an approximately hundredfold higher concentration...
Journal Articles
J Cell Sci (2002) 115 (14): 2985–2995.
Published: 15 July 2002
... fluorescent in situ hybridization( Moy and Silver, 1999 ). We originally performed this assay in strains lacking the Xrn1 exonuclease, which degrades the 5′ ITS1 fragment. In xrn1Δ cells, the 5′ ITS1 RNA is distributed throughout the cytoplasm because, in these cells, the small ribosomal subunit is exported...
Journal Articles
Journal Articles
J Cell Sci (1999) 112 (7): 1065–1076.
Published: 1 April 1999
... MAPK expression in those plant cells. In situ hybridization (ISH) showed the presence of MAPK transcripts in the three plant cell types studied, but levels were very low in quiescent cells compared to those in cycling cells. The quantification of labelling density of ISH signals strongly suggests...
Journal Articles
Journal Articles
J Cell Sci (1996) 109 (2): 379–385.
Published: 1 February 1996
..., the level of expression of each subunit varied with the epithelial cell type as demonstrated by in situ hybridization: epidermal and follicular keratinocytes express mostly α and γ subunits (while β was low); a prevalence of β and γ was observed in sweat glands. Thus, it appeared that two out of the three...
Journal Articles
J Cell Sci (1996) 109 (1): 119–129.
Published: 1 January 1996
... that these structures might also be involved in the regulation of the intranuclear migration of viral messenger RNA. In conclusion, the results of electron microscopic in situ hybridization of rDNA probes performed on Lowicryl sections of non-infected cells and HSV-1 infected cells reveal that the gold particle...
Journal Articles
J Cell Sci (1995) 108 (8): 2781–2790.
Published: 1 August 1995
... fluorescence in situ hybridization and immunofluorescence in conjunction with dual channel confocal laser scanning microscopy. Arginyl-tRNA synthetase, elongation factor 1a, ribosomal RNA, and myelin basic protein mRNA were all co-localized in granules in the processes, veins and membrane sheets of the cell...
Journal Articles
J Cell Sci (1995) 108 (7): 2565–2572.
Published: 1 July 1995
... patterns as seen by fluorescence in situ hybridization. Transcripts of the HCMV-IE transcription unit were shown to be present in a main nuclear signal in the form of a track or elongated dot and as small nuclear RNA signals that radiate from this site towards the cytoplasm. A similar distribution pattern...
Journal Articles
J Cell Sci (1995) 108 (5): 1863–1872.
Published: 1 May 1995
...Shermali Gunawardena; Elizabeth Heddle; Mary C. Rykowski ABSTRACT In situ hybridization has become a powerful technique for dissecting nuclear structure. By localizing nucleic acids with high precision, it is possible to infer the native structure of chromosomes, replication factories...
Journal Articles
J Cell Sci (1995) 108 (1): 97–103.
Published: 1 January 1995
...Reiko Ohara; Seiichi Hirota; Hitoshi Onoue; Shintaro Nomura; Yukihiko Kitamura; Kumao Toyoshima ABSTRACT The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells...
Journal Articles
J Cell Sci (1994) 107 (9): 2499–2508.
Published: 1 September 1994
... by Company of Biologists 1994 vascular development retina fibronectin laminin astrocyte gene expression in situ hybridization As was discussed earlier, fibronectin expression is directly related to astrocyte migration. The specific stimulus that guides astrocyte migration from the optic...
Journal Articles
J Cell Sci (1994) 107 (3): 703–708.
Published: 1 March 1994
...M. Jamilena; C. Ruiz Rejón; M. Ruiz Rejón ABSTRACT The origin of the B chromosome of Crepis capillaris has been studied by using in situ hybridization with different DNA probes. Genomic in situ hybridization (GISH) with DNA from plants with and without Bs as probes indicates that the B chromosome...
Journal Articles
J Cell Sci (1994) 107 (3): 377–386.
Published: 1 March 1994
... generated by the myofibril are transduced laterally into the extracellular matrix. We observed costameres developing in cultured skeletal muscles, grown in micromass culture from cells taken from embryonic chicken leg. We detected proteins by immunofluorescence and mRNA by in situ hybridization. Antibody...
Journal Articles
J Cell Sci (1994) 107 (3): 457–467.
Published: 1 March 1994
... of the cells could be taken into account, a method was developed to preserve the spatial organization of the root meristem. The ribosomal genes were detected by fluorescence in situ hybridization using a biotinylated cDNA probe. 3-D images of 81 nuclei, obtained by confocal scanning laser microscopy, were...
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Journal Articles
J Cell Sci (1994) 107 (2): 649–657.
Published: 1 February 1994
..., we used in situ hybridization to localize rRNAs encoded by the endosymbiont nucleus of the cryptomonad alga, Cryptomonas Φ. Transcripts of the endosymbiont rRNA gene were observed within the endosymbiont nucleus, and in the compartment thought to represent the endosymbiont’s cytoplasm. These results...