... of mRNA ex vivo in complex brain cells, such as astrocytes and microglia, in physiological and pathological conditions. Astrocytes Microglia Hippocampus mRNA In situ hybridization Immunofluorescence ImageJ APPswe/PS1dE9 mouse Alzheimer's disease GFAP Astrocytes are the most...

... into the oocyte nucleus became specifically localized in the nucleoli, and endogenous SRP RNA was also detected in oocyte nucleoli by RNA in situ hybridization. An initial step in the assembly of SRP involves the binding of the SRP19 protein to SRP RNA. When green fluorescent protein (GFP)-tagged SRP19 protein...

.... In situ hybridization showed that poly(A) mRNA is abundant and non-uniformly distributed in nearly all axons. The specific transcripts for β-actin and actin-depolymerizing factor (ADF) were also present and non-uniformly distributed in axons, with an approximately hundredfold higher concentration...

... fluorescent in situ hybridization( Moy and Silver, 1999 ). We originally performed this assay in strains lacking the Xrn1 exonuclease, which degrades the 5′ ITS1 fragment. In xrn1Δ cells, the 5′ ITS1 RNA is distributed throughout the cytoplasm because, in these cells, the small ribosomal subunit is exported...

... degradation pathway. * Author for correspondence (e-mail: ar@gene.com ) 22 09 1999 17 11 1999 © 1999 by Company of Biologists 1999 Hedgehog In situ hybridization Chromosomal localization Trans-activation The Hedgehogs (HH) constitute a family of secreted proteins...

... MAPK expression in those plant cells. In situ hybridization (ISH) showed the presence of MAPK transcripts in the three plant cell types studied, but levels were very low in quiescent cells compared to those in cycling cells. The quantification of labelling density of ISH signals strongly suggests...

... to extracellular matrix breakdown in the human endometrium, both in cultured and non-cultured samples. The source of MMP-1 was identified by in situ hybridization and by immunohistochemistry on serial sections. This was compared with the immunolocalization of other MMPs, steroid receptors, macrophages, and laminin...

..., the level of expression of each subunit varied with the epithelial cell type as demonstrated by in situ hybridization: epidermal and follicular keratinocytes express mostly α and γ subunits (while β was low); a prevalence of β and γ was observed in sweat glands. Thus, it appeared that two out of the three...

... that these structures might also be involved in the regulation of the intranuclear migration of viral messenger RNA. In conclusion, the results of electron microscopic in situ hybridization of rDNA probes performed on Lowicryl sections of non-infected cells and HSV-1 infected cells reveal that the gold particle...

... fluorescence in situ hybridization and immunofluorescence in conjunction with dual channel confocal laser scanning microscopy. Arginyl-tRNA synthetase, elongation factor 1a, ribosomal RNA, and myelin basic protein mRNA were all co-localized in granules in the processes, veins and membrane sheets of the cell...

... patterns as seen by fluorescence in situ hybridization. Transcripts of the HCMV-IE transcription unit were shown to be present in a main nuclear signal in the form of a track or elongated dot and as small nuclear RNA signals that radiate from this site towards the cytoplasm. A similar distribution pattern...

...Shermali Gunawardena; Elizabeth Heddle; Mary C. Rykowski ABSTRACT In situ hybridization has become a powerful technique for dissecting nuclear structure. By localizing nucleic acids with high precision, it is possible to infer the native structure of chromosomes, replication factories...

...Reiko Ohara; Seiichi Hirota; Hitoshi Onoue; Shintaro Nomura; Yukihiko Kitamura; Kumao Toyoshima ABSTRACT The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells...

... by Company of Biologists 1994 vascular development retina fibronectin laminin astrocyte gene expression in situ hybridization As was discussed earlier, fibronectin expression is directly related to astrocyte migration. The specific stimulus that guides astrocyte migration from the optic...

...M. Jamilena; C. Ruiz Rejón; M. Ruiz Rejón ABSTRACT The origin of the B chromosome of Crepis capillaris has been studied by using in situ hybridization with different DNA probes. Genomic in situ hybridization (GISH) with DNA from plants with and without Bs as probes indicates that the B chromosome...

... generated by the myofibril are transduced laterally into the extracellular matrix. We observed costameres developing in cultured skeletal muscles, grown in micromass culture from cells taken from embryonic chicken leg. We detected proteins by immunofluorescence and mRNA by in situ hybridization. Antibody...

... of the cells could be taken into account, a method was developed to preserve the spatial organization of the root meristem. The ribosomal genes were detected by fluorescence in situ hybridization using a biotinylated cDNA probe. 3-D images of 81 nuclei, obtained by confocal scanning laser microscopy, were...

...Marie-Claude Azum-Gélade; Jacqueline Noaillac-Depeyre; Michèle Caizergues-Ferrer; Nicole Gas ABSTRACT The distribution of the U3 small nuclear RNA during the cell cycle of the CHO cell line was studied by in situ hybridization using digoxigenin-labelled oligonucleotide probes. The location...

..., argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and compo- sition may...

..., we used in situ hybridization to localize rRNAs encoded by the endosymbiont nucleus of the cryptomonad alga, Cryptomonas Φ. Transcripts of the endosymbiont rRNA gene were observed within the endosymbiont nucleus, and in the compartment thought to represent the endosymbiont’s cytoplasm. These results...