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Keywords: high-pressure freezing
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Journal Articles
Maria Rosario Fernandez-Fernandez, Desire Ruiz-Garcia, Eva Martin-Solana, Francisco Javier Chichon, Jose L. Carrascosa, Jose-Jesus Fernandez
Journal:
Journal of Cell Science
J Cell Sci (2017) 130 (1): 83–89.
Published: 1 January 2017
.... To explore this possibility, we have examined neuronal subcellular architecture by performing three-dimensional (3D) electron tomography analysis of mouse brain tissue that had been preserved through high-pressure freezing. Here, we report that sequestration of neuronal cytoplasmic contents occurs...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2008) 121 (1): 19–27.
Published: 1 January 2008
.... How VWF tubules drive the formation of these uniquely shaped organelles, and how WPB density increases during maturation, has recently been revealed by EM analysis using high-pressure freezing and freeze substitution. In addition, an AP1/clathrin coat has been found to be essential to WPB formation...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2007) 120 (12): 2117–2125.
Published: 15 June 2007
... factor (VWF), is crucial to WPB function, and so we investigated further the relationship between VWF tubule formation and WPB formation in human umbilical vein endothelial cells (HUVECs). By using high-pressure freezing and freeze substitution before electron microscopy, we visualised VWF tubules...
Includes: Multimedia, Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2000) 113 (24): 4587–4603.
Published: 15 December 2000
...-72076 Tübingen, Germany. § Author for correspondence (e-mail: [email protected] ) 16 11 2000 13 10 2000 © 2000 by Company of Biologists 2000 Glycosylphosphatidylinositol-anchored protein Protein sorting High-pressure freezing Immunoelectron microscopy...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1993) 106 (3): 847–858.
Published: 1 November 1993
...M. E. Galway; P. J. Rennie; L. C. Fowke ABSTRACT An ultrastructural study of endocytosis has been made for the first time in protoplasts of a gymnosperm, white spruce ( Picea glauca ), fixed by high-pressure freezing and freeze substitution. Protoplasts derived from the WS1 line of suspension...