... Transactivation Growth factors The EGF receptor (EGFR, also known as erbB1 and HER1) tyrosine kinase system is vitally involved in normal physiological processes such as tissue morphogenesis and wound repair, because these events require the key cell responses of survival, proliferation and migration...

..., or with the growth factors, IGF-1 or BDNF, increases SIRPα phosphorylation and SHP-2 binding rapidly and transiently, via Src family kinase activation; phosphorylated SIRPα dissociates from the lipid microdomains. A cytoplasmic tail fragment of SIRPα (cSIRPα), when expressed in primary cortical neurons, also...

...Eugene Tkachenko; Esther Lutgens; Radu-Virgil Stan; Michael Simons Full activity of fibroblast growth factors (FGFs) requires their internalization in addition to the interaction with cell surface receptors. Recent studies have suggested that the transmembrane proteoglycan syndecan-4 functions...

... of endogenous UPA in mediating the mitogenic effects of platelet-derived growth factor (PDGF) and basic fibroblast growth factor(bFGF) on human vascular smooth muscle cells (SMC). Growth-arrested SMC constitutively expressed UPA, but UPA expression and secretion increased several times upon stimulation...

... such treatment ( Fig. 3 ). *Author for correspondence (e-mail: [email protected] ) 25 6 2001 © The Company of Biologists Limited 2001 2001 Growth factors Signal transduction Protein kinases Protein phosphatases Cell nucleus Upon stimulation of many types of cell surface...

...Laura A. Maile; Jane Badley-Clarke; David R. Clemmons ABSTRACT The disintegrin echistatin inhibits ligand occupancy of the αVβ3 integrin and reduces Insulin-like growth factor I (IGF-I) stimulated migration, DNA synthesis, and receptor autophosphorylation in smooth muscle cells. This suggests...

... mitogens growth factors immediate early genes delayed early genes cell cycle gene regulation cell metabolism DNA After exiting from mitosis, mammalian cells have the option to either start a new round of cell division or to leave the cell cycle and enter a resting state, usually referred...

...Andreas Sewing; Christiane Bürger; Sabine Brüsselbach; Christian Schalk; Frances C. Lucibello; Rolf Müller ABSTRACT We show that the cyclin D1 gene is regulated by a variety of growth factors in human diploid fibroblasts (WI- 38). Expression of cyclin D1 mRNA is low in quiescent WI-38 cells...

... in the developing mouse and to study the regulation of tenascin expression by growth factors in vitro. At postnatal day 1 tenascin mRNAs are abundant in regions of bone and cartilage formation, as well as in the ependymal layer of the central nervous system. Previous studies have demonstrated that transforming...

... and acid treatment. EC-FBS was inactivated by passage over a heparin-Agarose column. The column-bound activity could be eluted as a single peak at ∼1.0 M NaCl. Stimulation of pericyte growth was also achieved with platelet-derived growth factor (PDGF), acidic fibroblast growth factor (aFGF) and basic...

... of Biologists 1990 human hair follicles organ maintenance growth factors The hair follicle is composed of epithelial components (the matrix and outer root sheath) and dermal components (the dermal papilla and connective tissue sheath). Hair growth, which is effected by the division of the hair...

... released by entrapped platelets, such as platelet-derived growth factor. We suggest that the supernatant remaining after the fibrinogen-thrombin reaction could stimulate fibroblast replication, even in the absence of other blood components. To examine this hypothesis we expressed liquid from a fibrin clot...

...William J. Larochelle; Neill Giese; Mary May-Siroff; Keith C. Robbins; Stuart A. Aaronson ABSTRACT Human platelet-derived growth factor (PDGF) is a connective tissue cell mitogen comprising two related chains encoded by distinct genes. The B chain is the homolog of the v-sis oncogene product...

.... The expression of DIA/LIF in vitro is both developmentally programmed and controlled by the action of other growth factors, the most notable of which are members of the fibroblast growth factor family expressed by the stem cells themselves. This indicates that differentiation and proliferation in early...

... are tedious to perform, or methods based on uptake of radiolabelled thymidine, which may be prone to errors caused by precursor pool artefacts. We describe here an assay for estimating the number of adherent cells present in a microculture and its application to the study of growth factors. The assay depends...

...Clare M. Heyworth; Ian L. O. Ponting; T. Michael Dexter ABSTRACT Haemopoietic cell growth factors are normally assayed using unfractionated marrow cells (NBM). However, using this population it is difficult to distinguish between direct versus indirect effects, because of the low incidence...

...Robert F. Brooks; Peter N. Riddle ABSTRACT When the proliferation rate of Swiss 3T3 cells is decreased by limiting the availability of growth factors, cell cycle variability increases, as predicted by the transition probability model. Nevertheless, the transition probabilities would appear to play...

... and embryonic stem (ES) cells. EC cells have proved to be a useful source of embryonic growth factors. A potent mitogen, ECDGF has been isolated from EC cell conditioned medium. ECDGF appears to be a novel member of the heparin binding growth factor family. A remarkable feature of heparin binding growth factors...

...William L. Farrar; Stuart W. Evans; Annick Harel-Bellan; Douglas K. Ferris ABSTRACT Haemopoietic growth factors stimulate a number of consensus biochemical and molecular events regardless of the specificity detailed by unique ligand and receptor structures. Analysis of three distinct colony...

... in order to estimate the mitogenic response of each corneal cell type to externally supplied growth factors and hormones. It was found that the corneal endothelial cells could be stimulated to initiate DNA synthesis by exposure to epidermal growth factor (EGF). The stimulatory effect of this growth factor...