... or the ATP/ADP ratio following shutdown of ATP production. Similarly, we find that treatments that elevate or suppress actin dynamics do not alter the ATP/ADP ratio in growth cones, the subcellular domain with the highest actin dynamics in developing neurons. Collectively, the data indicate that actin...

...Paula G. Slater; Garrett M. Cammarata; Annika G. Samuelson; Alexandra Magee; Yuhan Hu; Laura Anne Lowery ABSTRACT It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms...

... underlying the roles of Shot and ACF7 in growth cone advance. Secondly, we found a novel role for ACF7 and Shot in regulating the actin cytoskeleton through their ability to control the formation of filopodia. This function in F-actin regulation requires EF-hand motifs and interaction with the translational...

...Anne K. Mongiu; Elizabeth L. Weitzke; Oleg Y. Chaga; Gary G. Borisy Neuronal growth cone advance was investigated by correlative light and electron microscopy carried out on chick dorsal root ganglion cells. Advance was analyzed in terms of the two principal organelles responsible for protrusive...

...Gabrielle Meyerson; Karl H. Pfenninger; Sven Påhlman ABSTRACT Nerve growth cones of primary neurons are highly enriched in the proto-oncogene product pp60 c-src . In order to investigate this molecule further in growing neuronal cells, growth cone and cell body fractions were prepared from human SH...

... mechanism for growth cone extension and show that the growth of filopodia, in particular, is unlikely to be driven by osmotically generated hydrostatic pressure. In contrast to the short-term effects on growth cone morphology, the slower elongation of the neuritic cylinder showed a consistent osmotic...

... behaviour neutrophils growth cones BHK fibroblasts Many tissue cells, in vitro , move over their substratum, be it a collagen gel or a plastic Petri dish. The phenomenon of cell locomotion has been studied intensively for many years, both as a topic of purely academic interest, and because...