... activity selectively localized in growth cones of undifferentiated neurites, whereas in developing axons it displayed a biphasic pattern, being low in nascent axons and high in elongating ones. RhoA–Rho kinase (ROCK) signaling prevented axon initiation but had no effect on elongation, whereas formin...

... cone ( Movie 1 ) after electroporation with mNG–TOCA-1 and the membrane-binding region of GAP43–RFP, showing localisation of mNG–TOCA-1 to (i) filopodia, (ii) advancing lamellipodia and (iii) inwardly-moving puncta in the growth cone body. Enlarged images show montages of the regions marked by dashed...

...Joseph Atherton; Melissa Stouffer; Fiona Francis; Carolyn A. Moores ABSTRACT Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone...

... and mediators of this interpolymer crosstalk, however, are inadequately understood. We identify Fmn2, a non-diaphanous-related formin associated with cognitive disabilities, as a novel regulator of cooperative actin–microtubule remodelling in growth cones of both chick and zebrafish neurons. We show that Fmn2...

... interests. 20 7 2020 24 2 2021 © 2021. Published by The Company of Biologists Ltd 2021 Summary: Axon regeneration drives selective localization of alternatively spliced Cdc42 mRNA isoform to PNS axons. Alternative splicing Axon regeneration Growth cone mRNA...

... ). Individual MT translocation events and rates were measured by producing montages from processed images and tracing internal fluorescent speckles over time. Velocities of five MTs extending from the growth cone T zone into the P domain were averaged to report individual MT velocities for each cell...

...Szilárd Szikora; István Földi; Krisztina Tóth; Ede Migh; Andrea Vig; Beáta Bugyi; József Maléth; Péter Hegyi; Péter Kaltenecker; Natalia Sanchez-Soriano; József Mihály ABSTRACT Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in the growth cone...

...Andreas Prokop; Robin Beaven; Yue Qu; Natalia Sánchez-Soriano Summary The extension of long slender axons is a key process of neuronal circuit formation, both during brain development and regeneration. For this, growth cones at the tips of axons are guided towards their correct target cells...

... and Gprin1 within growing neurites, axons, and growth cones; (iii) the ability of Gprin1 siRNA to decrease α7 expression at the cell surface or within the cell, reverse the effect of Bgtx on neurite growth, and attenuate interaction between α7 and GPC proteins. These findings confirm an important role...

...Sara Geraldo; Phillip R. Gordon-Weeks Interactions between dynamic microtubules and actin filaments are essential to a wide range of cell biological processes including cell division, motility and morphogenesis. In neuronal growth cones, interactions between microtubules and actin filaments...

... is unknown. The matrix-rigidity response of fibroblasts appears to differ from that of neuronal growth cones but it is unknown whether the rigidity detection mechanism or response pathway is altered. Here, we report that RPTPα is required for rigidity-dependent reinforcement of fibronectin (FN)-cytoskeleton...

... demonstrated that TN-R induces microprocesses along neurites and enlarged growth cones of tectal cells by interacting with the cell adhesion molecule contactin 1. Here, we describe competition and cooperation between TN-R, lecticans and contactin 1, and their functional consequences for tectal cells. Aggrecan...

...Ann M. Rajnicek; Louise E. Foubister; Colin D. McCaig Electric fields (EFs) resembling those in the developing and regenerating nervous systems steer growth cones towards the cathode. Requirements for actin microfilaments, microtubules and their interactions during EF growth cone steering have been...

...Xiaoxin X. Wang; Karl H. Pfenninger The `signal regulatory protein' SIRPα is an Ig superfamily, transmembrane glycoprotein with a pair of cytoplasmic domains that can bind the phosphatase SHP-2 when phosphorylated on tyrosine. SIRPα is prominent in growth cones of rat cortical neurons and located...

..., as well as non-neuronal cells, such as astrocytes and ependymal cells. CAD cells, a neuronal cell line, express both full-length and headless Myo10, and this endogenous Myo10 is present in cell bodies, neurites, growth cones and the tips of filopodia. To investigate the dynamics of the two forms of Myo10...

...Peter W. Grabham; Boris Reznik; Daniel J. Goldberg Extracellular cues control the rate and direction of growth of neuronal processes in large part by regulating the cytoskeleton of the growth cone. The actin filament network of the peripheral region is thought to be the primary target...

... kinase Growth cone Axon guidance Synapse Angiogenesis Rho Ras In 1987, a receptor was identified in a search for tyrosine kinases involved in cancer ( Hirai et al.,1987 ). The new receptor was named Eph, after the erythropoietin-producing hepatocellular carcinoma cell line from which its...

... at the growth cone. This report examines whether this is a regulated event controlled by growth factors. The Golgi complex and nascent PPVs of hippocampal neurons in culture were pulse-labeled with fluorescent ceramide. We studied the dynamics of labeled PPVs upon arrival at the axonal growth cone. In controls...

...Motohiro Nozumi; Hiroyuki Nakagawa; Hiroaki Miki; Tadaomi Takenawa; Shigeaki Miyamoto The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply...

.... Immunostaining revealed that cortactin was enriched in areas of membrane budding, growth cones, and in the cell cortex of immature neurons. With differentiation, intense punctate staining was also observed in an extraction-resistant cytosolic compartment of the soma and processes. More importantly, suppression...