...David B. Williams Calnexin and calreticulin are related proteins that comprise an ER chaperone system that ensures the proper folding and quality control of newly synthesized glycoproteins. The specificity for glycoproteins is conferred by a lectin site that recognizes an early oligosaccharide...

...Roland Le Borgne; Nathalie Planque; Patrick Martin; Frédérique Dewitte; Simon Saule; Bernard Hoflack The Quail Neuroretina clone 71 gene (QNR-71) is expressed during the differentiation of retinal pigmented epithelia and the epidermis. It encodes a type I transmembrane glycoprotein that shares...

... morphogenetic role can be achieved only via a regional differentiation of the protein distribution in the epiplasm. We have tried to demonstrate such differentiation by preparing an epiplasm extract, which consists predominantly of concanavalin A (ConA)-positive glycoproteins. This fraction, either untreated...

..., and a series of minor components of 62-18×10 3 M r . Based upon lectin labeling and glycosidase treatment, some of the latter have been identified as glycoproteins. Using affinity-purified antibodies specific for individual glycoproteins or groups of glycoproteins, we were able to localize them in situ...

.... On immunoblots, antiserum 15 labels the hydrophilic proteins that are extracted from the epiplasm by treatment with dilute acid solution and that are predominantly glycoproteins, four of which are labeled with Concanavalin A on Western blots. On Lowicryl thin sections, antiserum 15 labels the epiplasm uniformly...

...Stamatis C. Stamatoglou; Kate H. Sullivan; Staff An Johansson; Peter M. Bayley; Ian D. J. Burdett; R. Colin Hughes ABSTRACT We have compared the localization of integrin α 5 β 1 and AGp100 (apical glycoprotein of M r 110×10 3 ) in rat liver parenchyma and in primary hepatocyte cultures. Integrin α...

...E. P. Parrish; J. E. Marston; D. L. Mattey; H. R. Measures; R. Venning; D. R. Garrod ABSTRACT Metabolic labelling with [ 35 S]methionine and immu-noprecipitation with specific antibodies to bovine desmosomal glycoproteins 2 and 3 (dg2 and dg3: desmocollins) reveals a triplet of polypeptides of M r...

... of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are formed. As part of our investigation into the function of the peanut lectin-binding glycoproteins of cultured human keratinocytes, we have examined their expression at different stages...

...Christopher E. Turner; Mary R. Newton; David M. Shotton ABSTRACT The independent capping of the three major rat thymocyte glycoproteins, the leucocyte-common (L-C) antigen, the leucocyte sialoglycoprotein (LSGP) and Thy-1, was investigated using specific monoclonal antibodies. The capping of each...

...Jonathan C. R. Jones ABSTRACT An analysis of the concanavalinA binding polypeptide components of bovine tongue epithelial desmosomes reveals that in addition to the known desmosomal glycoproteins of 100/115K (the ‘desmocollins’), 140K and 160/165K (*desmoglein 1’) there is an uncharacterized...

...Jonathan C. R. Jones; Karen L. Vikstrom; Robert D. Goldman ABSTRACT We have prepared both monoclonal and polyclonal antibody preparations directed against the 160/165 ×10 3 M r glycoproteins (desmogleins) of bovine tongue epithelial desmosomes. The polyclonal antibody preparation recognizes...

...M. Kallajoki; I. Virtanen; J. Suominen ABSTRACT The surface membrane glycoprotein composition of human spermatozoa has been studied by introducing radioactive label into galactosyl (Gal) and N-acetylgalactosaminyl (GalNAc) residues by using the galactose oxidase/NaB 3 H 4 method. Triton X-100...

...Jean-François Riou; Thierry Darribère; Jean-Claude Boucaut ABSTRACT Identification of the cell surface glycoproteins is an essential requirement for elucidating the mechanisms that mediate intercellular adhesion and cell migration in gastrulating amphibian embryos. In our experiments...