... in any medium provided that the original work is properly attributed. Summary: Δ40p53 exerts tumor suppressor activity that is associated with upregulation of p53-target gene expression and induces senescence in hepatocellular carcinoma cell lines. Δ40p53 Gene targeting Liver cancer...

... assembly WD domain protein Gene targeting Cilia/flagella are present in most eukaryotic organisms, including protozoans and humans, and share a highly organized and conserved microtubule-based structure, the axoneme ( Sleigh, 1974 ). Intraflagellar transport (IFT) is a bi-directional process...

...-mail: [email protected] ) 31 7 2006 © The Company of Biologists Limited 2006 2006 Titin Muscle Myofibrillogenesis Gene targeting Titin, the largest protein so far described, is a long, filamentous molecule found in the sarcomeres of skeletal and cardiac muscle that has many...

...-mail: [email protected] ) 18 5 2006 © The Company of Biologists Limited 2006 2006 JUN siRNA Gene targeting Endothelial cells Retinal neovascularisation The angiogenic process involves a complex series of cellular events including microvascular sprouting, endothelial...

... for initiating or regulating myofibrillogenesis. We used a gene-targeting approach in cultured myoblasts to truncate the titin gene so that the kinase domain and other domains downstream of the kinase were not expressed. We recovered cells in which one allele was targeted. We found that these cells expressed...

... and implicate USF-1 as a transcriptional regulator of genes(e.g. PAI-1) involved in wound repair. * Author for correspondence (e-mail: [email protected] ) 9 7 2002 © The Company of Biologists Limited 2002 2002 Keratinocytes PAI-1 Reepithelialization Gene targeting...

... normally transported to organelles by AP-3. * Author for correspondence (e-mail: [email protected] ) 31 10 2000 14 09 2000 © 2000 by Company of Biologists 2000 Mouse mutant Gene targeting Hermansky-Pudlak Syndrome Lamp protein Lysosome Melanosome...

...-mail: [email protected] ) 17 02 1997 29 05 1997 © 1997 by Company of Biologists 1997 Gap junction Connexin43 Connexin45 Preimplantation development Mouse Intercellular coupling Immunogold labeling Confocal microscopy Gene targeting Gap junctional coupling...

... to dissect the molecular mechanisms which transduce the effects of these ligands, both in vivo and in isolated systems. One approach we are employing is gene targeting of retinoic acid receptors (RARs) and cellular retinoid-binding proteins to generate mice in which one or more of these genes has been...

... genes and gene targeting techniques. Here we review briefly the systems and methods being used in these efforts to dissect protozoan myosin I structure and function using molecular genetic approaches. © The Company of Biologists Limited 1991 1991 myosin I molecular cloning structure-function...

...D. J. Manstein; K. M. Ruppel; L. Kubalek; J. A. Spudich ABSTRACT © The Company of Biologists Limited 1991 1991 Dictyostelium discoideum cytoskeleton myosin gene targeting expression The eukaryote Dictyostelium discoideum is an attractive model organism for the study...