... µM Fura-2/AM (TEFLabs, Austin, TX, USA) for 45 minutes. Fura-2/AM-loaded cells were imaged using a fluorescence microscope described previously ( Malli et al., 2007 ). For CFP/YFP-based FRET experiments cells were excited at 440 nm (440AF21; Omega Optical, Brattleboro, VT) and emission was gathered...

... transmission of inositol 1,4,5-trisphosphate-mediated calcium waves in FURA-2-loaded monolayers of human HeLa cells expressing murine connexin26, -32 or -43. Gap junction-mediated cell coupling in different connexin- transfected HeLa cells was standardized by measuring the spreading of microinjected Mn 2...

...%. * Author for correspondence 15 09 1993 16 11 1993 © 1994 by Company of Biologists 1994 calcium fura-2 exocytosis ATP receptor PC12 cell It is becoming increasingly apparent that extracellular ATP plays an important role in cellular signalling. ATP has been shown...

...+ is particularly well suited to studying the nature of agonist-stimulated Ca 2+ entry because it quenches the fura-2 fluorescence on entering the cytosol. Furthermore, cells do not possess endogenous agonist-releaseable Mn 2+ stores, so any quench of the fluorescence unequivocally indicates that Mn 2+ has...

...Colin Brownlee; Ann L. Pulsford ABSTRACT Fura-2 has been used with digital image analysis to visualize and quantify the cytoplasmic Ca 2+ gradient in rhizoid cells of Fucus serratus . Ca 2+ was higher at the growing rhizoid tip in about 50-60% of cells studied to date. Considerable variation...

... of cytoplasmic streaming and for measurements of fluorescence of the calcium indicator fura-2 The resting potential across the plasma membrane of root hair cells was between −140 and − 160 mV in both species. Insertion of microelectrodes caused immediate cessation of cytoplasmic streaming; 1–4 min later...