... or glutaraldehyde fixation followed by freeze substitution. The subplasmalemmal cytoplasm of the smooth muscle cells of rat epididymal tubules and the podocyte processes of the mouse glomerular visceral epithelium were found to be composed of a fine network of irregular anastomosing strands. This network closely...

...M. E. Galway; P. J. Rennie; L. C. Fowke ABSTRACT An ultrastructural study of endocytosis has been made for the first time in protoplasts of a gymnosperm, white spruce (Picea glauca), fixed by high-pressure freezing and freeze substitution. Protoplasts derived from the WS1 line of suspension...

...G. J. Hyde; S. Lancelle; P. K. Hepler; A. R. Hardham ABSTRACT Rapid freezing and freeze substitution (RF-FS) have been used to re-examine the process by which the multinucleate sporangium of the Oomycetes, Phytophthora cinnamomi and P. palmivora , is subdivided into uninucleate zoospores...

...Toshio Kanbe; Issei Kobayashi; Kenji Tanaka ABSTRACT Changes in the ultrastructure of the fission yeast Schizosaccharomyces pombe during the cell division cycle were analyzed by three-dimensional reconstruction of serial section electron micrographs of freeze-substituted cells. Cytoplasmic vesicles...

... the appearance of being composed of a chain of vesicles. Utilizing rapid freeze fixation and freeze substitution, we have re-examined the ultrastructure of the generative cell wall complex from pollen tubes grown in vitro , and the differences are striking. The two plasma membranes are very smooth and closely...