...Estibaliz Gómez-de-Mariscal; Mario Del Rosario; Joanna W. Pylvänäinen; Guillaume Jacquemet; Ricardo Henriques ABSTRACT Fluorescence microscopy is essential for studying living cells, tissues and organisms. However, the fluorescent light that switches on fluorescent molecules also harms the samples...

.... Convolutional neural networks Content-aware image restoration Antibody labelling Noise2noise Cellular structures Fluorescence microscopy Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 Medical Research Council http://dx.doi.org/10.13039...

... the negative effects of IO for live imaging. Fluorescence microscopy Phototoxicity Cell migration Mitochondrial dynamics Microtubule dynamics Adhesion dynamics Natural Sciences and Engineering Research Council of Canada 10.13039/501100000038 493616-16 386084-12 Canadian...

...: Image co-localization analysis is widely used but often misunderstood. This Review discusses important considerations, including applications to super-resolution microscopy. Image analysis Manders Pearson Co-localization Fluorescence microscopy Gordon and Betty Moore Foundation...

... transport to the nuclear pore complex. Adenovirus Fluorescence microscopy Virus entry Microtubule Intracellular transport CRM1 Machine learning Swiss National Science Foundation 10.13039/501100001711 310030B_160316 Swiss National Science Foundation 10.13039/501100001711...

... ). The dysregulated activation of STAT signalling is involved in chronic inflammation ( O'Sullivan et al., 2007 ) and cancer, including blood malignancies and solid tumours ( Yu and Jove, 2004 ). JAK-STAT signalling STAT3 Dimerization Inflammatory hepatocellular adenoma Hyper-IgE syndrome Fluorescence...

... stabilization in the cell periphery, we investigated the peripheral trafficking itineraries and kinetics of wild type (WT) and F508del in living cells using high-speed fluorescence microscopy together with fluorogen activating protein detection. We directly visualized internalization and accumulation of CFTR WT...

... subunit of SOCE channels, Orai1. Fluorescence microscopy revealed that NAGly did not affect STIM1 oligomerization, STIM1 clustering, or the colocalization of STIM1 with Orai1, which were induced by Ca 2+ depletion of the endoplasmic reticulum. In contrast, independently from its slow depolarizing effect...

... ) 4 7 2011 © 2011. 2011 Neutral lipid Lipolysis Lipid droplet Endoplasmic reticulum Fluorescence microscopy Electron microscopy Tomography Saccharomyces cerevisiae Lipid droplets form the main lipid storage organelles for neutral lipids in eukaryotic cells...

... at the time of exocytosis. To determine whether this interaction occurs, we studied the precise site of exocytosis by examining the exocytotic events in vivo using total internal reflection fluorescence microscopy (TIRFM) observation of granules from cells expressing GFP–synaptobrevin-II and compared...

... of drug addition is a deterministic decision. Fluorescence microscopy Protease Caspase Lineage Epigenetics Apoptosis is a form of cell death that eliminates unwanted or harmful cells ( Ellis et al., 1991 ). It is characterized by the activation of caspases ( Salvesen and Dixit, 1997 ; Yuan...

..., USA 12 8 2008 © The Company of Biologists Limited 2008 2008 Endocytosis Axolemma Synaptic vesicle Trafficking Fluorescence microscopy In the nervous system, axons are guided to their specific targets by highly motile growth cones, present at their distal tip, which...

.... ‡ Author for correspondence (e-mail: [email protected] ) * Present address: Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA 27 4 2006 © The Company of Biologists Limited 2006 2006 Actin Dnm1 Dynamin Fluorescence microscopy...

...-repressor SMRT Nuclear structure Green fluorescent protein Fluorescence microscopy Pit-1 Coactivator and co-repressor protein complexes control gene expression by modifying the acetylation states of histones and other transcriptional regulators (for reviews, see Orphanides and Reinberg, 2002...

... dynamics of this phenomenon is limited. In this report, the effect of the activation of H-Ras on its microdomain localization was studied by single-molecule fluorescence microscopy. Individual human H-Ras molecules fused to the enhanced yellow fluorescent protein (eYFP) were imaged in the dorsal plasma...

... is a multi-step process requiring the novel integral membrane component Fis1p. J. Cell Biol. 151 , 367 -379. Nagorni, M. and Hell, S. W. ( 2001 ). Coherent use of opposing lenses for axial resolution increase in fluorescence microscopy. II. Power and limitation of nonlinear image restoration. J. Opt...

... antibiotic, filipin, in combination with high resolution fluorescence microscopy, intense fluorescent staining characteristically decorated the periphery of triglyceride droplets (TGD) as well as the plasma membrane (PM) of fat cells. Filipin-staining was not visible inside the lipid droplets. Purification...

.... * Author for correspondence at present address: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA 22 09 1999 19 07 1999 © 1999 by Company of Biologists 1999 Fluorescence microscopy Cytoskeleton Mechanochemistry Tubulin Molecular...

... of Biologists 1999 Microtubule dynamics Centrosome Diffusion Fluorescence microscopy Melanophore Microtubules (MTs) are dynamic polymers in the sense that they are continuously being built and degraded in living cells. This MT turnover most likely plays a key role in cellular processes...

...Ivan A. Vorobjev; Tatyana M. Svitkina; Gary G. Borisy ABSTRACT The origin of non-centrosomal microtubules was investigated in a variety of animal cells in culture by means of time-lapse digital fluorescence microscopy. A previous study ( Keating et al. (1997) Proc. Nat. Acad. Sci. USA 94, 5078-5083...