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Keywords: fluorescence
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Journal Articles
In collection:
Cytoskeleton
J Cell Sci (2022) 135 (3): jcs258745.
Published: 2 February 2022
...Aaron R. Tipton; Gary J. Gorbsky ABSTRACT The microtubules of the mitotic spindle mediate chromosome alignment to the metaphase plate, then sister chromatid segregation to the spindle poles in anaphase. Previous analyses of spindle microtubule kinetics utilizing fluorescence dissipation after...
Includes: Supplementary data
Journal Articles
In collection:
Mitochondria
J Cell Sci (2017) 130 (24): 4193–4199.
Published: 15 December 2017
...Christopher Estell; Emmanouela Stamatidou; Sarah El-Messeiry; Andrew Hamilton ABSTRACT Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using...
Includes: Supplementary data
Journal Articles
J Cell Sci (2017) 130 (24): 4193–4199.
Published: 15 December 2017
...Christopher Estell; Emmanouela Stamatidou; Sarah El-Messeiry; Andrew Hamilton ABSTRACT Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2017) 130 (15): 2644–2653.
Published: 1 August 2017
...Eugenio Gallo; Jonathan W. Jarvik ABSTRACT A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small-molecule fluorogens. In this report, we investigated the property of multi-selectivity activation by a single antibody...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2017) 130 (15): 2644–2653.
Published: 1 August 2017
...Eugenio Gallo; Jonathan W. Jarvik ABSTRACT A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small-molecule fluorogens. In this report, we investigated the property of multi-selectivity activation by a single antibody...
Includes: Supplementary data
Journal Articles
In collection:
Adhesion , Imaging
J Cell Sci (2016) 129 (15): 2897–2904.
Published: 1 August 2016
.... ( 2007 ). Multicolor super-resolution imaging with photo-switchable fluorescent probes . Science   317 , 1749 - 1753 . 10.1126/science.1146598 Berika , M. and Garrod , D. ( 2014 ). Desmosomal adhesion in vivo . Cell Commun. Adhes.   21 , 65 - 75 . 10.3109/15419061.2013.876018...
Includes: Supplementary data
Journal Articles
In collection:
Adhesion , Imaging
J Cell Sci (2016) 129 (15): 2897–2904.
Published: 1 August 2016
... of proteins in the desmosome, a structure mediating strong cell–cell adhesion, is elucidated by dSTORM super-resolution fluorescence microscopy. Cell adhesion Keratinocyte Super-resolution Fluorescence Microscopy Cadherin dSTORM National Institutes of Health 10.13039/100000002...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (2): 288–294.
Published: 15 January 2014
...Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong V. Wang ABSTRACT We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement...
Journal Articles
J Cell Sci (2013) 126 (17): 3805–3815.
Published: 1 September 2013
... to contribute to future solutions. * Author for correspondence ( timm.schroeder@bsse.ethz.ch ) © 2013. Published by The Company of Biologists Ltd 2013 Biosensors Fluorescence Hardware Single-cell imaging Software Time-lapse microscopy Organisms are composed of vast numbers...
Journal Articles
J Cell Sci (2013) 126 (13): 2810–2819.
Published: 1 July 2013
.... It is especially true in cells where the spatiotemporal distribution of Tau–MT interactions is unknown. Using Förster resonance energy transfer (FRET), we showed that the Tau–MT interaction was distributed along MTs in periodic hotspots of high and low FRET intensities. Fluorescence recovery after photobleaching...
Includes: Supplementary data
Journal Articles
Series: Imaging Series
J Cell Sci (2010) 123 (21): 3621–3628.
Published: 1 November 2010
...Alexa L. Mattheyses; Sanford M. Simon; Joshua Z. Rappoport Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma...
Includes: Multimedia, Supplementary data
Journal Articles
Series: Imaging Series
J Cell Sci (2010) 123 (21): 3629–3638.
Published: 1 November 2010
... for correspondence ( maddy.parsons@kcl.ac.uk ) © 2010. 2010 Cell adhesion Fluorescence Focal adhesion Live imaging Microscopy Migration Several different types of adhesions are formed by cells following engagement with the extracellular matrix (ECM). These structures are classified on the basis...
Journal Articles
J Cell Sci (2007) 120 (14): 2413–2423.
Published: 15 July 2007
... for correspondence (e-mail: tamkunmm@lamar.colostate.edu ) 9 5 2007 © The Company of Biologists Limited 2007 2007 Cytoskeletal fence Fluorescence Live cell imaging Localization Potassium channel Quantum dot tracking Fig. 9. Concentration-dependent effects of swinholide A on GFP...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (3): 643–650.
Published: 1 February 2005
... by a donkey anti-goat RRX secondary antibody. The collected images show 100% colocalization with the receptors in addition to some background fluorescence. To measure the distribution of the BMP receptors on the cell surface, we used image correlation spectroscopy (ICS) and ICCS. A431 cells were cultured...
Journal Articles
Journal Articles
J Cell Sci (2003) 116 (14): 2833–2838.
Published: 15 July 2003
...Jeffrey M. Levsky; Robert H. Singer Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific nucleic acids sequences in native context, is a 20-year-old technology that has developed continuously. Over its maturation,various methodologies and modifications have...
Journal Articles
J Cell Sci (2002) 115 (13): 2725–2733.
Published: 1 July 2002
...Pamela K. Hanson; Althea M. Grant; J. Wylie Nichols At low temperature, the short-chain fluorescent-labeled phospholipids,1-myristoyl-2-[6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl]-phosphatidylcholine (M-C 6 -NBD-PC) and its phosphatidylethanolamine analog, M-C 6 -NBD-PE, are internalized...
Journal Articles
J Cell Sci (1993) 1993 (Supplement_17): 165–169.
Published: 1 December 1993
...-tails promise to give new insights into the stability and lifetime of membrane domains. © The Company of Biologists Limited 1993 1993 plasma membrane lateral diffusion fluorescence FPR domains For many years my laboratory’s work has focused on problems concerning the lateral...
Journal Articles
J Cell Sci (1989) 93 (2): 363–374.
Published: 1 June 1989
...Richard G. Sleight; Marina N. Abanto ABSTRACT The transport and metabolism of a fluorescent phosphatidylcholine analog, l-palmitoyl-2-(N-4-nitrobenzo-2-oxa-l,3-diazole)-aminocaproyl-phos-phatidylcholine ((palmitoyl, C 6 -NBD)-PC), in BHK, CH0-K1, CHO-15B, MDCK, VA-2, Vero, V79 and WI-38 cells has...
Journal Articles