... miRNA Mouse Nicotinic acetylcholine receptors nAChR Cell culture Fluorescence Neuromuscular junction Dystrophin complex National Institute of Neurological Disorders and Stroke http://dx.doi.org/10.13039/100000065 R01NS111041 University of Michigan http://dx.doi.org...

... and antibodies outlined in Black et al. (2022) . Secondary antibody fluorescence was detected using the LI-COR Odyssey ® SA Infrared imaging system. Band intensities on high-quality composite images were then quantified using ImageStudioLite (Version 5.2) ( Fig. S2 ). Mean pixel grey values of proteins...

...Aaron R. Tipton; Gary J. Gorbsky ABSTRACT The microtubules of the mitotic spindle mediate chromosome alignment to the metaphase plate, then sister chromatid segregation to the spindle poles in anaphase. Previous analyses of spindle microtubule kinetics utilizing fluorescence dissipation after...

...Christopher Estell; Emmanouela Stamatidou; Sarah El-Messeiry; Andrew Hamilton ABSTRACT Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using...

...Eugenio Gallo; Jonathan W. Jarvik ABSTRACT A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small-molecule fluorogens. In this report, we investigated the property of multi-selectivity activation by a single antibody...

... of proteins in the desmosome, a structure mediating strong cell–cell adhesion, is elucidated by dSTORM super-resolution fluorescence microscopy. Cell adhesion Keratinocyte Super-resolution Fluorescence Microscopy Cadherin dSTORM National Institutes of Health 10.13039/100000002...

...Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong V. Wang ABSTRACT We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement...

... to contribute to future solutions. * Author for correspondence ( [email protected] ) © 2013. Published by The Company of Biologists Ltd 2013 Biosensors Fluorescence Hardware Single-cell imaging Software Time-lapse microscopy Organisms are composed of vast numbers...

.... It is especially true in cells where the spatiotemporal distribution of Tau–MT interactions is unknown. Using Förster resonance energy transfer (FRET), we showed that the Tau–MT interaction was distributed along MTs in periodic hotspots of high and low FRET intensities. Fluorescence recovery after photobleaching...

...Alexa L. Mattheyses; Sanford M. Simon; Joshua Z. Rappoport Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma...

... for correspondence ( [email protected] ) © 2010. 2010 Cell adhesion Fluorescence Focal adhesion Live imaging Microscopy Migration Several different types of adhesions are formed by cells following engagement with the extracellular matrix (ECM). These structures are classified on the basis...

... for correspondence (e-mail: [email protected] ) 9 5 2007 © The Company of Biologists Limited 2007 2007 Cytoskeletal fence Fluorescence Live cell imaging Localization Potassium channel Quantum dot tracking Voltage-gated K + (Kv) channels play a key role in establishing...

... in serum starved A431 cells. ICCS is an extension of ICS, where a cross-correlation function is generated by calculating the coincident fluorescence-intensity-fluctuations in images collected from two different chromophores that are attached to two different protein or receptor types. One receptor...

... of Biologists Limited 2003 2003 Image correlation spectroscopy Fluorescence BMP receptors Receptor clusters Smad pathway Bone morphogenetic proteins (BMPs) and growth differentiation factors(GDFs) belong to the TGF-β superfamily( Hogan, 1996 ; Bhatia et al., 1999 ; Celeste et al., 1990...

...Jeffrey M. Levsky; Robert H. Singer Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific nucleic acids sequences in native context, is a 20-year-old technology that has developed continuously. Over its maturation,various methodologies and modifications have...

...Pamela K. Hanson; Althea M. Grant; J. Wylie Nichols At low temperature, the short-chain fluorescent-labeled phospholipids,1-myristoyl-2-[6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl]-phosphatidylcholine (M-C 6 -NBD-PC) and its phosphatidylethanolamine analog, M-C 6 -NBD-PE, are internalized...

...-tails promise to give new insights into the stability and lifetime of membrane domains. © The Company of Biologists Limited 1993 1993 plasma membrane lateral diffusion fluorescence FPR domains For many years my laboratory’s work has focused on problems concerning the lateral...

...Richard G. Sleight; Marina N. Abanto ABSTRACT The transport and metabolism of a fluorescent phosphatidylcholine analog, l-palmitoyl-2-(N-4-nitrobenzo-2-oxa-l,3-diazole)-aminocaproyl-phos-phatidylcholine ((palmitoyl, C 6 -NBD)-PC), in BHK, CH0-K1, CHO-15B, MDCK, VA-2, Vero, V79 and WI-38 cells has...

... as stemming from a closer cell–substratum apposition beneath the lamellae than the cell body. This ambiguity can be avoided if the technique of total internal reflection aqueous fluorescence (TIRAF) is used in conjunction with a high refractive index glass (n = 1·83) as substratum. Contributions to the image...