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Keywords: flow cytometry
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Journal Articles
J Cell Sci (2022) 135 (5): jcs258322.
Published: 20 July 2021
..., inexpensive and non-destructive identification of lipid content, and highlights the differences in lipid biology between cell types by imaging and flow cytometry. References Ahlabo , I. and Barnard , T. ( 1971 ). Observations of peroxisomes in brown adipose tissue of the rat . J. Histochem...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (5): jcs238014.
Published: 05 March 2020
.... Activation-induced gains in fluorescence were also observed in naïve and CD4 + T cells from the Lifeact-EGFP reporter, and were readily detected by both flow cytometry and live cell microscopy. This unique, translationally controlled reporter of effector T cell activation simultaneously enables tracking...
Journal Articles
J Cell Sci (2018) 131 (6): jcs213736.
Published: 21 March 2018
...Thomas D. Williams; Robert R. Kay ABSTRACT Macropinocytosis is a conserved endocytic process used by Dictyostelium amoebae for feeding on liquid medium. To further Dictyostelium as a model for macropinocytosis, we developed a high-throughput flow cytometry assay to measure macropinocytosis...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (2): jcs202416.
Published: 29 January 2018
... during S phase progression. High-resolution data reveal the culmination of replication in the mid S phase in the nucleolus. A. thaliana rDNA Replication Nucleolus Flow cytometry Structured illumination microscopy Ribosomal RNA genes (rDNA) are organised into long repetitive clusters...
Includes: Supplementary data
Journal Articles
In collection:
Autophagy
J Cell Sci (2016) 129 (24): 4622–4632.
Published: 15 December 2016
... of autophagy using Acridine Orange, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of Acridine-Orange-stained cells. This method measured with accuracy the increase in autophagy induced by starvation or rapamycin, and the reduction...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (2): 473–485.
Published: 15 January 2012
... sperm and that ENaCs are blocked during capacitation. In the present work, we used flow cytometry to analyze changes in intracellular Na + concentration ([Na + ] i ) during capacitation in individual cells. Our results indicate that capacitated sperm have lower Na + concentrations. Using sperm...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (10): 2131–2140.
Published: 15 April 2004
...Zakaria Hmama; Khalid Sendide; Amina Talal; Rosa Garcia; Karen Dobos; Neil E. Reiner Macrophage cell membranes were labeled with PKH26 and subsequently incubated with latex beads to generate phagosomes surrounded by a red-fluorescent membrane suitable for flow cytometry. Following cell disruption...
Journal Articles
J Cell Sci (1993) 106 (1): 1–9.
Published: 01 September 1993
...Miriam J. Smyth; Rodney L. Sparks; Walker Wharton *Author for correspondence © 1992 by Company of Biologists 1992 differentiation proliferation adipogenesis growth arrest adipocyte-specific gene expression flow cytometry REFERENCES Ailhaud G. ( 1982 ). Adipose...
Journal Articles
J Cell Sci (1992) 102 (1): 71–78.
Published: 01 May 1992
... on the other. Key words: PCNA, DNA/nuclear proteins, flow cytometry. Introduction There is increasing interest in studies on the regulatory mechanisms of the cell cycle. The ability of cells to begin a new cell cycle is a prerequisite for cell reproduction (Pardee, 1989). To study the transition of cells from...
Journal Articles
J Cell Sci (1987) 88 (5): 579–590.
Published: 01 December 1987
... castellanii cell cycle encystation anti-BrdUrd labelling flow cytometry The cell cycle and its relationship to development in Acanthamoeba castellanii MICHAEL STOHR Instittit fiir Experiiiienlelle I'citlmlogie, Deutsches Krebsforsdningszentrum, lm Neuenheimer Feld 2S0, 6900 Heidelberg, West Geivumv...