...A. Scherberich; R. P. Tucker; E. Samandari; M. Brown-Luedi; D. Martin; R. Chiquet-Ehrismann We cloned and characterized a novel member of the tenascin family of extracellular matrix proteins - the murine orthologue of zebrafish tenascin-W. Full-length recombinant tenascin-W was expressed...

...Axel Völker; York-Dieter Stierhof; Gerd Jürgens The Arabidopsis KNOLLE gene encodes a cytokinesis-specific syntaxin that localises to the plane of division and mediates cell-plate formation. KNOLLE mRNA and protein expression is tightly regulated during the cell cycle. To explore the significance...

..., Ole e I has been immunolocalized for the first time within the pollen wall and in the tapetum. Reverse transcription-polymerase chain reaction analysis using specific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards...

... the size limit and the intensity of DNA nuclear transport could be increased by the attachment of strong nuclear localization signals. Conjugation of a 900 bp expression cassette to nuclear localization signals increased both its nuclear entry and expression in microinjected, living cells. * Author...

... that N-myristoyl transferase may be recruited to the membrane as part of a translational complex, perhaps by binding to p34 ribosome binding protein, a leucine rich repeat receptor of the microsomal membranes. We have also studied the expression pattern of the gene in the embryo by northern blot analysis...

... found in bovine tissues. We have reported previously that one of these, DSC3, is expressed only in basal and lower suprabasal layers of stratified epithelia. Using RT-PCR we have now obtained the complete cDNA coding sequence of mature bovine DSC3. It has alternatively spliced ‘a’ and ‘b’ forms found...

...Kin-Chow Chang; Kenneth Fernandes; Margaret J. Dauncey ABSTRACT Members of the myosin heavy chain (MyHC) gene family show developmental stage- and spatial-specificity of expression. We report on the characterization and identification of a porcine skeletal fast MyHC gene, including its...

...V. L. Misener; C.-c. Hui; I. A. Malapitan; M.-E. Ittel; A. L. Joyner; J. Jongstra ABSTRACT Mouse LSP1 is a 330 amino acid intracellular F-actin binding protein expressed in lymphocytes and macrophages but not in non-hematopoietic tissues. A 328 amino acid LSP1-related protein, designated S37...

...Julie A. Chambers; Michael A. Hollingsworth; Ann E. O. Trezise; Ann Harris ABSTRACT The mucin gene MUC1 , is expressed in a number of human ductal epithelia in vivo including those within the pancreas, mammary gland, kidney and genital ducts. Further it is expressed at a high level in certain...

...Simon J. Atkinson; Murray Stewart ABSTRACT We have expressed in Escherichia coli a cDNA clone corresponding broadly to rabbit light meromyosin (LMM) together with a number of modified polypeptides and have used this material to investigate the role of different aspects of molecular structure...

...Robert D. Moir; Anne D. Donaldson; Murray Stewart ABSTRACT We have expressed in Escherichia coli cDNA corresponding to human lamins A and C, together with a number of fragments produced using site-specific mutagenesis. The proteins produced in this way were characterised both biochemically...

...Michael Way; Brian Pope; Alan Weeds ABSTRACT We review the impact of molecular biology on actin binding proteins, in particular on sequence relationships and expression of clones to dissect properties in vitro . Significant homologies exist between proteins in each class, but we propose...

... of expressed LMM cDNA indicated that charge zone complementation is not a major driving force in myosin polymerisation. Instead, it probably serves to prevent unfavourable interaction geometries. © The Company of Biologists Limited 1991 1991 myosin assembly structure mutagenesis expression...

...D. J. Manstein; K. M. Ruppel; L. Kubalek; J. A. Spudich ABSTRACT © The Company of Biologists Limited 1991 1991 Dictyostelium discoideum cytoskeleton myosin gene targeting expression The eukaryote Dictyostelium discoideum is an attractive model organism for the study...

...Roy A. Quinlan; Robert D. Moir; Murray Stewart ABSTRACT We have expressed in Escherichia coli a 1258 bp cDNA fragment corresponding to 97% of mouse glial fibrillary acidic protein (GFAP), the principal intermediate filament protein of astrocytes. High levels of expression were obtained, as a fusion...