.... Inhibition of ROCK activity in ARHGAP32 -knockout (KO) cells effectively restored desmosomal organization and the integrity of epithelial cell sheets. Moreover, loss of DSP impaired desmosomal ARHGAP32 location and led to decreased actomyosin contractility. ARHGAP32 with a deletion of the GAB2-ID domain...

...-junction-based barrier function during continual mechanical compression due to peristalsis. Another fascinating possibility comes from studies of the Caenorhabditis elegans intestine, which identified gap-junction-mediated Ca 2+ waves in intestinal epithelial cells as essential for coordinating rhythmic...

... on the cell-type-specific mechanisms regulating ciliogenesis at the apical surface in polarized epithelial cells, which are particularly relevant for many ciliopathies. Furthermore, recent findings have demonstrated the importance of actin cytoskeleton dynamics in positively and negatively regulating multiple...

... and Tsp2A, and is required for the correct localization of these proteins to sSJs. Knockdown of hoka in the adult midgut leads to intestinal barrier dysfunction and stem cell overproliferation. In hoka -knockdown midguts, aPKC is upregulated in the cytoplasm and the apical membrane of epithelial cells...

...Yasushi Izumi; Kyoko Furuse; Mikio Furuse ABSTRACT Smooth septate junctions (sSJs) contribute to the epithelial barrier, which restricts leakage of solutes through the paracellular route in epithelial cells of the Drosophila midgut. We previously identified three sSJ-associated membrane proteins...

.... It is also unclear how cellular forces, cell–cell adhesion and velocities are coordinated within streams. To independently tune stiffness and collagen fiber length, we developed new hydrogels and discovered invasion-like streaming of normal epithelial cells on soft substrates coated with long collagen fibers...

... abundant and diverse cytoskeletal system in epithelial cells ( Windoffer et al., 2011 ), and during the process of EMT endogenous expression of keratin is downregulated in favor of vimentin expression ( Boyer et al., 2000 ; Nieto et al., 2016 ; Savagner, 2010 ; Tan et al., 2014 ). Previous studies have...

... within different cells. Circadian clock Epithelial cell Fibroblast Breast Lung Epidermis Circadian gene expression Medical Research Council 10.13039/501100000265 G0900414 Biotechnology and Biological Sciences Research Council 10.13039/501100000268 Wellcome...

... inhibited it. Inhibitors of focal adhesion kinase and Cdc42 had negligible effects. Hence, initial actin polymerization occurs in the lateral membrane, and is primarily important to initiate dead cell exfoliation and cell migration to close the gap. Recently three-dimensional (3D) primary epithelial cell...

.... , Panja , A. , Kaplan-Liss , E. , Mayer , L. and Raicht , R. F. ( 1995 ). Expression of growth factor receptor-encoded mRNA by colonic epithelial cells is altered in inflammatory bowel disease . Dig. Dis. Sci.   40 , 485 - 494 . 10.1007/BF02064355 Andrade , D...

...-to-mesenchymal transition (EMT) share certain superficial similarities, but their congruence – or lack thereof – remains unclear. In this Commentary, we discuss aspects of cell jamming, its established role in human epithelial cell layers derived from the airways of non-asthmatic and asthmatic donors, and its...

... role of Cdc42 in ureteric bud and metanephric mesenchyme development is to regulate epithelial cell polarity and the actin cytoskeleton. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 10 10 2014...

... limitations, as human sperm flagellum is positive for hSAXO1 along its full length, making it proportionally more abundant compared with its expression in other cell types. Basal body localization was also observed at the base of motile cilia of human bronchial epithelial cells; however, no signal could...

... (EDAC), in the neighboring normal cells, and vice versa. These results indicate that EPLIN is a crucial regulator of the interaction between normal and transformed epithelial cells. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing...

...Yukako Oda; Tetsuhisa Otani; Junichi Ikenouchi; Mikio Furuse ABSTRACT When the surface view of each epithelial cell is compared with a polygon, its sides correspond to cell–cell junctions, whereas its vertices correspond to tricellular contacts, whose roles in epithelial cell morphogenesis have...

... and reproduction in any medium provided that the original work is properly attributed. Epithelial cell Mesenchymal cell Fibrillin-1 Fibronectin Perlecan Integrin Syndecan Cell–cell junction Fibrillin, the main component of microfibrils of the extracellular matrix (ECM), supports elastic fibre...

... . * Author for correspondence ( [email protected] ) 25 6 2012 © 2012. Published by The Company of Biologists Ltd 2012 Drosophila Midgut Epithelial cell Smooth septate junction Epithelia play important roles as barriers that separate distinct compartments within the body...

...Minji Kim; Anirban Datta; Paul Brakeman; Wei Yu; Keith E. Mostov Epithelial cells are polarized, with an apical surface facing a lumen or outer surface and a basolateral surface facing other cells and extracellular matrix (ECM). Hallmarks of epithelial carcinogenesis include loss of polarity...

... and gelatinase-B (MMP-9) and in the collagen invasive activity of A549 cells. Further, we found that epilysin and the recombinant hemopexin domain were targeted to the surface of epithelial cells. This cell surface interaction was sensitive to the proteolytic activity of MT1-MMP, and was lost after EMT. Current...

... of this transporter complex in polarized epithelial cells. CD98 also interacts with β1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal...