...Lee Dolat; Raphael H. Valdivia ABSTRACT Our understanding of how the obligate intracellular bacterial pathogen Chlamydia trachomatis reprograms the function of infected cells in the upper genital tract is largely based on observations made in cell culture with transformed epithelial cell lines...

... function and is localized to the apical junctional complex in polarized epithelial cells. Earlier, we identified two distinct conserved motifs on the Na,K-β 1 transmembrane domain that mediate protein-protein interactions: a glycine zipper motif involved in the cis homo-oligomerization of Na,K-β 1...

... elucidate these mechanisms, we generated a conditional knockout of DG in the epithelial compartment of the mouse mammary gland. Deletion of DG caused an inhibition of mammary epithelial outgrowth and a failure of lactation. Surprisingly, loss of DG in vivo did not disrupt normal tissue architecture or BM...

...Jacqueline Whyte; Laura Thornton; Sara McNally; Sarah McCarthy; Fiona Lanigan; William M. Gallagher; Torsten Stein; Finian Martin Mammary epithelial cells organize in three dimensions and generate acini when supported on laminin-rich extracellular matrix. Acinus formation begins with the apicobasal...

... stores, is through the regulation of cytosolic [Ca 2+ ] within a microdomain. Exocytosis Calcium Epithelial Zymogen Pancreatic Two photon Ca 2+ -dependent exocytosis is an essential and widespread process (Sudhof, 2004). An increase in cytosolic Ca 2+ concentration ([Ca 2+ ]) triggers...

... resistance that contributes to luminal filling in three-dimensional mammary epithelial acinar structures in vitro. Given that integrins and growth factor receptors are highly interdependent for function, we examined the role of integrin subunits in ErbB2-mediated survival signaling. Here, we show that MCF...

...Laura E. Lamb; Beatrice S. Knudsen; Cindy K. Miranti The androgen receptor (AR) is expressed in differentiated secretory prostate epithelial cells in vivo. However, in the human prostate, it is unclear whether androgens directly promote the survival of secretory cells, or whether secretory cells...

...Mari Inada; Antonia Follenzi; Kang Cheng; Manju Surana; Brigid Joseph; Daniel Benten; Sriram Bandi; Hong Qian; Sanjeev Gupta Understanding the biological potential of fetal stem/progenitor cells will help define mechanisms in liver development and homeostasis. We isolated epithelial fetal human...

...M. Lynn Weir; Maria Luisa Oppizzi; Michael D. Henry; Akiko Onishi; Kevin P. Campbell; Mina J. Bissell; John L. Muschler Precise contact between epithelial cells and their underlying basement membrane is crucial to the maintenance of tissue architecture and function. To understand the role...

...Le Shen; Eric D. Black; Edwina D. Witkowski; Wayne I. Lencer; Vince Guerriero; Eveline E. Schneeberger; Jerrold R. Turner Epithelial tight junctions form a barrier against passive paracellular flux. This barrier is regulated by complex physiologic and pathophysiologic signals that acutely fine-tune...

...Christopher J. Watson; Catherine J. Hoare; David R. Garrod; Gordon L. Carlson; Geoffrey Warhurst Impairment of the gut epithelial barrier by agents such as IFNγ may play a key role in the pathogenesis of inflammatory disorders by increasing the paracellular penetration of luminal macromolecules...

... Epithelial Constriction One of the most intensely studied populations of morphogenic epithelia is that which gives rise to the adult brain and spinal chord, as defects in neural tube closure effect approximately 0.1% of human births, resulting in spina bifida and anencephaly (Campbell et al., 1986...

... coprecipitates with ankyrin-3-containing complexes isolated from Madin Darby canine kidney epithelial cells and phosphorylates this epithelial ankyrin isoform in vivo. These results are the first demonstration of a kinase constitutively associating with the ankyrin-spectrin cytoskeleton in erythroid and kidney...

...Harmeet Malhi; Adil N. Irani; Singh Gagandeep; Sanjeev Gupta The liver can regenerate itself through the progenitor cells it harbors. Here we demonstrate isolation of epithelial progenitor/stem cells from the fetal human liver, which contains a large number of hepatoblasts. Progenitor liver cells...

... of newly synthesized galactosphingolipids to the two surface domains of epithelial cells . J. Cell Biol . 132 , 813 – 821 . 10.1083/jcb.132.5.813 van der Bliek , A. M. , Kooiman , P. M. , Schneider , C. and Borst , P. (1988). Sequence of mdr3 cDNA encoding a human P...

... a previously unrecognized function of adseverin in epithelial cell function. Fig. 1. Characterization of anti adseverin antibodies. 50 μg of total protein extract from mouse kidney, small intestine, and brain and indicated amounts of purified recombinant murine adseverin were loaded per lane and subjected...

...Feodor Berdichevsky; Deborah Alford; Brendan D’Souza; Joyce Taylor-Papadimitriou ABSTRACT To study the morphogenesis of human epithelial cells in vitro we have used a three-dimensional collagen matrix and a newly developed mammary epithelial cell line, 1-7 HB2. In standard medium 1-7 HB2 cells...

... dependent upon the quality of the medium and constituents used. Cultures reached confluence within 10–14 days and consisted of epithelial colonies together with varying amounts of smooth-muscle-like cells. Cultures have been maintained for periods up to one month, but the longer-term potential for growth...