... complex pharmacological target for diseases with aberrant angiogenesis as its effects are strongly dependent on its phosphorylation status and simultaneous activity of other intracellular signaling pathways. Angiogenesis Endothelial cell STAT3 IL-6 family cytokines Oncostatin M Ciliary...

...Chandrani Sarkar; Debanjan Chakroborty; Sandeep Goswami; Hao Fan; Xiaokui Mo; Sujit Basu ABSTRACT We have previously demonstrated significant upregulation of dopamine D2 (DAD2) receptor (DRD2) expression on tumor endothelial cells. The dopamine D2 receptors, upon activation, inhibit...

.... Biosensor Rho GTPase Endothelial cell Thrombin Rho Nederlandse Organisatie voor Wetenschappelijk Onderzoek http://dx.doi.org/10.13039/501100003246 ALWOP.306 Universiteit van Amsterdam http://dx.doi.org/10.13039/501100001827 Rho GTPases function as a molecular switch...

.... Evans ABSTRACT Endothelial cell (EC) sensing of fluid shear stress direction is a critical determinant of vascular health and disease. Unidirectional flow induces EC alignment and vascular homeostasis, whereas bidirectional flow has pathophysiological effects. ECs express several mechanoreceptors...

... platelets perfused over the uncoated apical surface and induce platelet aggregation. Similar microthrombi were observed when endothelial cells (ECs) were co-cultured on the apical surface. Confocal imaging shows podoplanin-expressing MSCs extending processes into the EC layer, and these processes could...

...Jiahui Cao; Hans Schnittler ABSTRACT Junction dynamics of endothelial cells are based on the integration of signal transduction, cytoskeletal remodeling and contraction, which are necessary for the formation and maintenance of monolayer integrity, but also enable repair and regeneration. The VE...

..., also known as GJA1) with human umbilical vein endothelial cells (HUVECs), through which miR-200b could transfer from BMSCs to HUVECs to regulate osteogenesis and angiogenesis. As a negative regulator, the decrease in miR-200b level in BMSCs derepressed the expression of VEGF-A, leading to increased...

... YAP Endothelial cell Deutsche Forschungsgemeinschaft 10.13039/501100001659 SBF1032 Morphogenetic processes and the remodeling of existing tissue require the spatiotemporal integration of extracellular mechanical information alongside biochemical guidance cues. The translation...

... standing wave fields (USWFs) were employed to non-invasively and volumetrically pattern endothelial cells within 3D collagen hydrogels. Patterned hydrogels were composed of parallel bands of endothelial cells located at nodal regions of the USWF and spaced at intervals equal to one half wavelength...

... development of collaterals. Here, we report the role of AGS8 in vessel formation by endothelial cells. Knockdown of AGS8 by small interfering RNA (siRNA) inhibited vascular endothelial growth factor (VEGF)-induced tube formation, as well as VEGF-stimulated cell growth and migration. VEGF stimulated...

...Kiyomi Tsuji-Tamura; Minetaro Ogawa ABSTRACT Endothelial cell morphology needs to be properly regulated during angiogenesis. Vascular endothelial growth factor (VEGF) induces endothelial cell elongation, which promotes sprouting of pre-existing vessels. However, therapeutic angiogenesis using VEGF...

...Jui M. Dave; Colette A. Abbey; Camille L. Duran; Heewon Seo; Gregory A. Johnson; Kayla J. Bayless ABSTRACT During angiogenesis, endothelial cells must coordinate matrix proteolysis with migration. Here, we tested whether the focal adhesion scaffold protein Hic-5 (also known as TGFB1I1) regulated...

... proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of PPARγ in PMVEC functions, such as angiogenesis...

...Ana C. P. Correia; Jan-Renier A. J. Moonen; Marja G. L. Brinker; Guido Krenning ABSTRACT Endothelial-to-mesenchymal transition (EndMT) is characterized by the loss of endothelial cell markers and functions, and coincides with de novo expression of mesenchymal markers. EndMT is induced by TGFβ1...

... in specific cell–cell contact sites of endothelial cells and suppresses LPA signalling through the LPA 6 receptor (also known as LPAR6). In HEK293 cells, overexpression of LPP3 dramatically suppressed activation of LPA 6 . In human umbilical vein endothelial cells (HUVECs), LPA induced actin stress fibre...

... are well defined, whereas the purpose of RhoC remains poorly understood. Here, we describe how RhoC promotes vascular homeostasis by modulating endothelial cell migration, proliferation and permeability. RhoC stimulates proliferation of human umbilical vein endothelial cells (HUVECs) by stabilizing nuclear...

... allows for potential paracrine signaling between them and the underlying non-epithelial stromal cells. In support of this, we have previously demonstrated that endothelial cells support growth of basal cells during co-culture through vascular endothelial growth factor A (VEGFA)-mediated signaling...

... receptor (FGFR1), subsequently promoting FGF2–FGFR1 activation and signaling. Using bioinformatic approaches, both GPC1 and FGFR1 were identified and subsequently validated as targets for miR-149 (both the mature strand, miR-149, and the passenger strand, miR-149*) in endothelial cells (ECs...

...Emma A. Cookson; Ianina L. Conte; John Dempster; Matthew J. Hannah; Tom Carter Summary Regulated secretion from endothelial cells is mediated by Weibel–Palade body (WPB) exocytosis. Plasma membrane cholesterol is implicated in regulating secretory granule exocytosis and fusion pore dynamics...

... that the transmembrane chemokine CX3CL1 (fractalkine) is induced in inflamed lymphatic endothelium, both in vitro in TNF-α-treated human dermal lymphatic endothelial cells (HDLECs) and in vivo in a mouse model of skin hypersensitivity. However, unlike blood endothelial cells, which express predominantly transmembrane...