... glutamine and its metabolism by glutaminases. Addition of ammonia, a metabolite of glutamine, was sufficient to enlarge vacuoles via PIKFYVE inhibition. Moreover, PIKFYVE inhibition led to intracellular ammonium accumulation. Endosome–lysosome permeabilization resulted in ammonium leakage from the cells...

... is required for VPS4A stability and its early endosomal localization. Either loss of PPTC7 or presence of constitutively phosphorylated VPS4A leads to defective recycling of EGFR, thus leading to EGFR re-routing to lysosomes for degradation. Further, we demonstrate that PPTC7-VPS4A-dependent EGFR recycling...

...Stéphane Vassilopoulos; Guillaume Montagnac ABSTRACT Clathrin assembles into honeycomb-like lattices at the plasma membrane but also on internal membranes, such as at the Golgi and tubular endosomes. Clathrin assemblies primarily regulate the intracellular trafficking of different cargoes...

... distinct transport vesicles at the trans -Golgi network (TGN), and the epsin-related proteins Ent3p and Ent5p (collectively Ent3p/5p) act as accessories for these adaptors. Recently, we showed that vesicle transport from the TGN is crucial for yeast Rab5 (Vps21p)-mediated endosome formation, and that Ent3p...

... several proteins involved in membrane trafficking. Here, we set out to identify novel substrates of this protein. We found that endogenously tagged Yck3 localized not only at the vacuole, but also on endosomes. To disable Yck3 function, we generated a kinase-deficient mutant and thus identified the I-BAR...

...Shumpei Nakashima; Takahide Matsui; Mitsunori Fukuda ABSTRACT The small GTPase Rab22A is an important regulator of the formation of tubular endosomes, which are one of the types of recycling endosome compartments of the clathrin-independent endocytosis pathway. In order to regulate tubular endosome...

... and endosomes following internalization, endosomal trafficking of EGFR spatiotemporally regulates EGFR signaling. In this process, leucine-rich repeat kinase 1 (LRRK1) has multiple roles in kinase activity-dependent transport of EGFR-containing endosomes and kinase-independent sorting of EGFR...

... homolog, Bph1. We show that Bph1 is an endosomal protein and an effector of the minor Rab5 isoform Ypt52. Strikingly, bph1 Δ mutant cells have lipidated Atg8 on their endosomes, which is sorted via late endosomes into the vacuole lumen under non-autophagy-inducing conditions. In agreement...

... and extracellular matrix adhesion during migration has not been investigated. Here, we reveal a novel role for ALFY in the endocytic pathway and in cell migration. We show that ALFY localizes to RAB5- and EEA1-positive early endosomes in a PtdIns(3)P-dependent manner and is highly enriched in cellular protrusions...

... reticulum (ER)-to-Golgi trafficking was severely inhibited. Moreover, we discovered a novel Rab1-depletion phenotype: perinuclear clustering of early endosomes and delayed transferrin recycling. In contrast, acute Rab5 protein depletion resulted in loss of early endosomes and late endosomes, but lysosomes...

... provided that the original work is properly attributed. Highlighted Article: Endocytic delivery to lysosomes by kiss and run/membrane fusion entails pore formation commencing at the edge of tether arrays, and demonstrates SNARE redundancy and compensation. Lysosome Endosome Membrane...

...Anthony Ravussin; Andreas Brech; Sharon A. Tooze; Harald Stenmark ABSTRACT Late endosomes and lysosomes (endolysosomes) receive proteins and cargo from the secretory, endocytic and autophagic pathways. Although these pathways and the degradative processes of endolysosomes are well characterized...

... through the ER, Ca 2+ is transported towards membrane contact sites with mitochondria or endolysosomes, where it plays key roles in the regulation of essential organellar biology, which are reviewed here. Calcium ER Membrane contact sites Mitochondria Endosome Owing to its distinct...

...Mark Remec Pavlin; James H. Hurley ABSTRACT The endosomal sorting complexes required for transport (ESCRTs) I, -II and –III, and their associated factors are a collection of ∼20 proteins in yeast and ∼30 in mammals, responsible for severing membrane necks in processes that range from multivesicular...

...Ashley J. Evans; James L. Daly; Anis N. K. Anuar; Boris Simonetti; Peter J. Cullen ABSTRACT Human retromer, a heterotrimer of VPS26 (VPS26A or VPS26B), VPS35 and VPS29, orchestrates the endosomal retrieval of internalised cargo and promotes their cell surface recycling, a prototypical cargo being...

...Lena Dehnen; Maren Janz; Jitender Kumar Verma; Olympia Ekaterini Psathaki; Lars Langemeyer; Florian Fröhlich; Jürgen J. Heinisch; Heiko Meyer; Christian Ungermann; Achim Paululat ABSTRACT Endosome biogenesis in eukaryotic cells is critical for nutrient uptake and plasma membrane integrity. Early...

...Dalton Buysse; Anna-Katharina Pfitzner; Matt West; Aurélien Roux; Greg Odorizzi ABSTRACT The ESCRT-III protein complex executes reverse-topology membrane scission. The scission mechanism is unclear but is linked to remodeling of ESCRT-III complexes at the membrane surface. At endosomes, ESCRT-III...

... with decreased cellular copper export as a result of disruption of the ATP7B trafficking itinerary and early endosome accumulation when COMMD1 is depleted. PtdIns(4,5)P 2 interaction with COMMD1 as well as COMMD1 abundance could both be important in maintenance of specific membrane protein trafficking pathways...

...Junya Hasegawa; Imen Jebri; Hikaru Yamamoto; Kazuya Tsujita; Emi Tokuda; Hideki Shibata; Masatoshi Maki; Toshiki Itoh ABSTRACT Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies...

... CORVET, CHEVI and HOPS are major organizers of the endocytic pathway. This Review combines current knowledge on their roles in membrane trafficking and diseases. Disease Endosome HOPS Lysosome Membrane trafficking Tethering CHEVI CORVET ZonMw 10.13039/501100001826 40-00812-98...