...Itziar Pinilla-Macua; Sachin Surve; Alexander Sorkin Ligand binding to EGFR activates Rho family GTPases, triggering actin cytoskeleton reorganization, cell migration and invasion. Activated EGFR is also rapidly endocytosed but the role of EGFR endocytosis in cell motility is poorly understood...

... endocytic adaptor 1 SGIP1 Cannabinoid receptor 1 Endocannabinoid system Endocytosis Presynapse Synaptic transmission Wellcome Trust http://dx.doi.org/10.13039/100010269 220799/Z/20/Z Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268...

...Emma Evergren; Ian G. Mills; Grace Kennedy ABSTRACT Membrane trafficking, a fundamental cellular process encompassing the transport of molecules to specific organelles, endocytosis at the plasma membrane and protein secretion, is crucial for cellular homeostasis and signalling. Cancer cells adapt...

... contributes to its functional diversity. Historically, clathrin was identified by electron microscopy (EM) as the main component of the protein coat that lines the cytosolic face of small plasma membrane (PM) invaginations involved in cell surface receptor endocytosis ( Goldstein et al., 1979 ; Heuser...

... Riemer; Tuguldur Tumurbaatar; Kristina Djinovic-Carugo; Gang Dong; Christian J. Janzen; Brooke Morriswood ABSTRACT All endocytosis and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of the plasma membrane. This subdomain, the flagellar pocket, is a small vase-shaped...

... inhibitor, in Saccharomyces cerevisiae . Unexpectedly, we found that surface levels of most proteins examined were either unaffected or increased during myriocin treatment, consistent with an observed decrease in bulk endocytosis. In contrast, sphingolipid depletion triggered selective endocytosis...

... and pathological processes. Cadherins undergo dynamic intracellular trafficking, and their surface level is determined by a balance between endocytosis, recycling and degradation. However, the regulatory mechanism of cadherin turnover in collective cell migration remains elusive. In this study, we show...

... positioning of cargoes from the earliest stages of endocytosis and through the two main intracellular itineraries: (1) degradation at the lysosome or (2) recycling back to the plasma membrane. In vitro and cellular ( in vivo ) studies on cargo transport thus far have typically focussed independently on either...

..., the reduction in dynamin-1 is not the single cause of neurodegeneration of these neurons. The reduction in levels of dynamin-1 protein and mRNA, as well as of other endocytosis proteins, in Stxbp1 null mutant neurons suggests that MUNC18-1 directly or indirectly controls expression of other presynaptic genes...

...Daniel M. Williams; Lale Gungordu; Anthony Jackson-Crawford; Martin Lowe ABSTRACT Endocytosis allows cells to internalise a wide range of molecules from their environment and to maintain their plasma membrane composition. It is vital during development and for maintenance of tissue homeostasis...

...Vartika Sharma; Bappi Sarkar; Mousumi Mutsuddi; Ashim Mukherjee ABSTRACT Deltex (Dx) is a context-dependent regulator of Notch signaling that can act in a non-canonical fashion by facilitating the endocytosis of the Notch receptor. In an RNAi-based modifier screen of kinases and phosphatases, we...

... the process of CD166 uptake. We also reveal that the Rho GTPase Rac1, but not Cdc42, is a master regulator of this endocytic route. Finally, we provide evidence that microtubules and kinesin molecular motors are required to potentiate endoA3-dependent endocytosis. Of note, our study also highlights potential...

...- and C-termini of Jen1 in its biogenesis, PM stability and transport activity, using functional analyses of Jen1 truncations and chimeric constructs with UapA, an endocytosis-insensitive transporter of Aspergillus nidulans . Our results show that both N- and C-termini are critical for Jen1 trafficking...

... by weakening the lipid bilayer. Endocytosis Multivesicular body Intraluminal vesicles Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659 KO 963/8-1 Endosomal sorting complex required for transport (ESCRT)-III proteins are membrane remodeling factors...

... with this, proteomic analysis of bph1 Δ vacuoles reveals an accumulation of Atg8, reduced flux via selective autophagy, and defective endocytosis. Additionally, bph1 Δ cells have reduced autophagic flux under starvation conditions. Our observations suggest that Bph1 is a novel Rab5 effector that maintains endosomal...

..., depletion of Snazarus (Snz) led to decreased autophagic flux. Interestingly, we observed altered distribution of Vamp7-positive vesicles with Snz depletion, and the roles of Snz were conserved in human cells. SNX25, the closest human ortholog to Snz, regulates both VAMP8 endocytosis and lipid metabolism...

... of the AP2 adaptor complex, a key player in clathrin-mediated endocytosis, is a highly organized process controlled by alpha- and gamma-adaptin-binding protein (AAGAB, also known as p34). In this study, we demonstrate that besides AP2, AAGAB also regulates the assembly of AP1, a cargo adaptor involved...

... D'Angelo , G. , Matusek , T. , Pizette , S. and Thérond , P. P. ( 2015 ). Endocytosis of Hedgehog through dispatched regulates long-range signaling . Dev. Cell 32 , 290 - 303 . 10.1016/j.devcel.2014.12.004 Delevoye , C. , Miserey-Lenkei , S. , Montagnac , G...

.../caveolae-mediated but not clathrin-mediated endocytosis, on its tautomerase activity and, probably, on its thiol protein oxidoreductase activity. As MIF treatment restrained actin polymerisation in vitro , our findings establish a new intracellular role for MIF/D-DT in driving cell motility through...

...Matthias Corrotte; Mark Cerasoli; Fernando Y. Maeda; Norma W. Andrews ABSTRACT Endocytosis of caveolae has previously been implicated in the repair of plasma membrane wounds. Here, we show that caveolin-1-deficient fibroblasts lacking caveolae upregulate a tubular endocytic pathway and have...