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Keywords: electron microscopy
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Journal Articles
In collection:
Membrane trafficking
J Cell Sci (2024) 137 (8): jcs261674.
Published: 24 April 2024
... and the accompanying poster, we review our current knowledge on the different types of canonical and non-canonical membrane-associated clathrin assemblies in mammalian cells, as observed by thin-section or platinum replica electron microscopy in various cell types, and discuss how the structural plasticity of clathrin...
Includes: Supplementary data
Journal Articles
J Cell Sci (2022) 135 (6): jcs259313.
Published: 30 March 2022
... distance, as measured by electron microscopy, with ionic fluxes. We found that native cER cisternae extended during store depletion and remained elongated at a constant ER-plasma membrane (PM) gap distance during subsequent Ca 2+ elevations. Tethering proteins enhanced store-dependent cER expansion...
Includes: Supplementary data
Journal Articles
J Cell Sci (2022) 135 (5): jcs258325.
Published: 11 August 2021
... of this process are intensively studied, while information about how the cell responds structurally to heat stress is mostly lacking. Here, Saccharomyces cerevisiae were subjected to a mild continuous heat shock (38°C) and intermittently cryo-immobilised for electron microscopy. Through measuring changes in all...
Includes: Supplementary data
Journal Articles
Journal Articles
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2021) 134 (10): jcs258603.
Published: 24 May 2021
...Sandrine Pizette; Tamás Matusek; Bram Herpers; Pascal P. Thérond; Catherine Rabouille ABSTRACT In metazoans, tissue growth and patterning is partly controlled by the Hedgehog (Hh) morphogen. Using immuno-electron microscopy on Drosophila wing imaginal discs, we identified a cellular structure...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (10): jcs216754.
Published: 22 May 2018
..., collectively called the ‘switchblade model’. This hypothesis, however, has not been extensively tested for other classes of integrins. We prepared a set of recombinant integrin ectodomain fragments including αvβ3, α2β1, α3β1, α5β1, α6β1 and α6β4, and used negative-stain electron microscopy to examine...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (10): jcs216754.
Published: 22 May 2018
..., collectively called the ‘switchblade model’. This hypothesis, however, has not been extensively tested for other classes of integrins. We prepared a set of recombinant integrin ectodomain fragments including αvβ3, α2β1, α3β1, α5β1, α6β1 and α6β4, and used negative-stain electron microscopy to examine...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (4): jcs210450.
Published: 22 February 2018
...Mark Terasaki ABSTRACT The endoplasmic reticulum (ER) is an interconnected network of tubules and sheets. In most tissues of the body, ER tubules have a diameter of ∼60 nm. Using new methods for serial-section electron microscopy, a distinct class of very narrow, 20- to 30-nm-diameter tubules were...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (4): jcs210450.
Published: 22 February 2018
...Mark Terasaki ABSTRACT The endoplasmic reticulum (ER) is an interconnected network of tubules and sheets. In most tissues of the body, ER tubules have a diameter of ∼60 nm. Using new methods for serial-section electron microscopy, a distinct class of very narrow, 20- to 30-nm-diameter tubules were...
Includes: Supplementary data
Journal Articles
Journal Articles
Journal Articles
J Cell Sci (2016) 129 (2): 444–456.
Published: 15 January 2016
... precluded structural analysis. Because it is difficult to capture rare and transient events, only a few attempts have been made to observe specific developmental and pathological processes in animal models using electron microscopy. The multimodal correlative approach that we propose here combines...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (8): 1568–1579.
Published: 15 April 2015
... channels in ER‐plasma membrane clusters. A longer splice variant, STIM1L, forms permanent ER‐plasma membrane clusters and mediates rapid Ca 2+ influx in muscle. Here, we used electron microscopy, total internal reflection fluorescence (TIRF) microscopy and Ca 2+ imaging to establish the trafficking...
Includes: Supplementary data
Journal Articles
In collection:
Lipid biology
J Cell Sci (2015) 128 (8): 1627–1638.
Published: 15 April 2015
... or financial interests. 10 9 2014 2 2 2015 © 2015. Published by The Company of Biologists Ltd 2015 Plasma membrane Lipid asymmetry Microdomain Electron microscopy Freeze fracture The asymmetric distribution of lipids between the outer and inner leaflet of the membrane...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2015) 128 (7): 1269–1278.
Published: 1 April 2015
... no competing or financial interests. © 2015. Published by The Company of Biologists Ltd 2015 Caveolae Caveolin Cavin Coiled coil Electron microscopy X-ray crystallography Caveolae (or ‘little caves’) are bulb-shaped invaginations of 50–60 nm that are a main feature of the plasma...
Journal Articles
In collection:
Lipid biology
J Cell Sci (2014) 127 (22): 4801–4812.
Published: 15 November 2014
... extensively with traditional light and electron microscopy to determine the subcellular localization of lipids and their spatial and temporal regulation. We highlight some of the recent studies that have investigated the distribution of lipids and their ability to cluster using super-resolution and electron...
Journal Articles
J Cell Sci (2013) 126 (20): 4545–4552.
Published: 15 October 2013
...Graham Knott; Christel Genoud Summary Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed...
Journal Articles
J Cell Sci (2012) 125 (11): 2571–2580.
Published: 1 June 2012
... (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM...
Journal Articles
In collection:
Lipid biology
J Cell Sci (2011) 124 (22): 3894–3904.
Published: 15 November 2011
... fundamental questions as to the biogenesis, subcellular distribution and inheritance of lipid droplets are as yet unsolved. In this study, we applied a set of imaging techniques such as high-resolution four-dimensional (4D) live-cell imaging, quantitative microscopy, transmission electron microscopy...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2011) 124 (21): 3676–3683.
Published: 1 November 2011
... preferentially transcribed a network of KLF1-regulated genes. Here we used correlative light microscopy in combination with energy filtering transmission electron microscopy (EFTEM) and electron microscopy in situ hybridization (EMISH) to analyse transcription factories, transcribing genes, and their nuclear...
Includes: Supplementary data