...Nikolai I. Kiskin; Nicola Hellen; Victor Babich; Lindsay Hewlett; Laura Knipe; Matthew J. Hannah; Tom Carter Using fluorescence recovery after photobleaching (FRAP) we measured the mobilities of EGFP-tagged soluble secretory proteins in the endoplasmic reticulum (ER) and in individual Weibel–Palade...

... of the signaling membrane and induces long-term adaptation. However, the molecular mechanism underlying TRPL translocation remains unclear. Here we report that eGFP-tagged TRPL expressed in the photoreceptor cells formed functional ion channels with properties of the native channels, whereas TRPL-eGFP...

... by producing a series of enhanced green fluorescent protein (eGFP)-tagged MKLP1 and its variant mutations, and studying the distribution of these molecules in cultured primary hippocampal neurons using fluorescence microscopy. We have found that: (a) full-length MKLP1(1-856)-eGFP was distributed...

... in vitro and as a new marker for Parkinson's disease and other synucleopathies. In this paper the structural and functional consequences of TPPP/p25 are characterized to elucidate the relationship between the in vitro and the pathological phenomena. We show that at low expression levels EGFP-TPPP/p25...

...-types in vivo is still unclear, especially under minimal injury conditions. In this study, we describe a method of purifying murine mesenchymal stem cells from bone marrow and efficiently transducing them using a lentivirus vector expressing the eGFP reporter gene. Lentivirus-transduced mesenchymal stem...

...Nibedita Lenka; Zhong J. Lu; Philipp Sasse; Jürgen Hescheler; Bernd K. Fleischmann To gain insight into early events of neurogenesis, transgenic embryonic stem (ES) cells were generated using the enhanced green fluorescence protein( EGFP ) reporter gene under the regulatory control of the neural...

... domains of PTP-BL in epithelial MDCK cells, that the presence of a FERM domain in the protein is both necessary and sufficient for its targeting to the apical side of epithelial cells. Furthermore, immuno-electron microscopy on stable expressing MDCK pools, that were obtained using an EGFP-based cell...

... of Biologists 1999 Membrane skeleton Focal adhesion Vinculin Alpha-actinin Lamin EGFP Membrane skeletons are networks of cytoskeletal and membrane proteins that constitute transmembrane linkages between the extracellular surface and the underlying cortical cytoskeleton. Such specialized...