... transport (IFT)88, the small GTPase Arl13b, as well as subunits of axonemal dynein. Key parameters in sample preparation, immunolabeling and imaging acquisition are discussed to facilitate similar studies by others in the cilia research community. * Present address: Stanford Cryo-Electron Microscopy...

... tracks. Dynein and kinesins govern autophagosome trafficking on microtubules through the sequential recruitment of their effector proteins, post-translational modifications and interactions with LC3-interacting regions (LIRs). In contrast, myosins are actin-based motors that participate in various stages...

... conserved mechanism to ensure that mitosis occurs in a timely manner. We identified many RNA transcripts that encode proteins involved in mitosis localized at the mitotic spindles in dividing sea urchin embryos and mammalian cells. Disruption of microtubule polymerization, kinesin-1 or dynein results...

.... Published by The Company of Biologists Ltd 2023 Summary: In this Review, we discuss recent studies to highlight what is known about the regulation of endosomal vesicle positioning and transport by motors and cargo adaptors. Cytoskeleton Membrane trafficking Motor proteins Dynein Kinesin...

...Reto Gassmann ABSTRACT The microtubule minus-end-directed motility of cytoplasmic dynein 1 (dynein), arguably the most complex and versatile cytoskeletal motor, is harnessed for diverse functions, such as long-range organelle transport in neuronal axons and spindle assembly in dividing cells...

... endosomal choreography, which includes bidirectional and stop-and-go motions, is coordinated by the microtubule-based motor proteins dynein and kinesin. These motors bridge various endosomal subtypes to the microtubule tracks thanks to their cargo-binding domain interacting with endosome-associated proteins...

... called the fibrous corona as microtubule attachments form. This shedding is mediated, in part, by the minus-end directed motor dynein, which ‘strips’ cargoes along K-fibre microtubules. Despite its essentiality, little is known about how dynein stripping is regulated and how it responds to attachment...

.... Biol. 25 , 200 - 205 . 10.1016/j.cub.2014.11.008 Del Castillo , U. , Winding , M. , Lu , W. and Gelfand , V. I. ( 2015b ). Interplay between kinesin-1 and cortical dynein during axonal outgrowth and microtubule organization in Drosophila neurons . Elife 4 , e10140...

... Summary: A DNA molecule folded in a shape of a spring is used to measure forces generated by dynamic microtubule ends, and to record single-molecule fluorescence at the site of force generation. DNA origami Force sensor Microtubule Dynein Kinetochore Optical trap European Molecular...

...Kari H. Ecklund; Megan E. Bailey; Kelly A. Kossen; Carsten K. Dietvorst; Charles L. Asbury; Steven M. Markus ABSTRACT Dynein motors move the mitotic spindle to the cell division plane in many cell types, including in budding yeast, in which dynein is assisted by numerous factors including...

... demonstrated to be dependent on the proteasome. We show that BBS1 regulates this process by coupling the 19S proteasome regulatory subunit to the microtubule motor dynein for its transport to the centrosome. Our data identify the ciliopathy-related protein BBS1 as a new player in T cell synapse assembly...

...Stephen M. King ABSTRACT Axonemal dyneins power the beating of motile cilia and flagella. These massive multimeric motor complexes are assembled in the cytoplasm, and subsequently trafficked to cilia and incorporated into the axonemal superstructure. Numerous cytoplasmic factors are required...

... in building the cilium by shuttling structural components and signaling receptors between the ciliary base and tip. To provide effective transport, IFT-A and IFT-B adaptor protein complexes assemble into highly repetitive polymers, called IFT trains, that are powered by the motors kinesin-2 and IFT-dynein...

... with mitochondria – the actin-based myosins, as well as the microtubule-based kinesins and dynein. In addition, Miro and TRAK proteins act as adaptors that link kinesin-1 and dynein, as well as myosin of class XIX (MYO19), to mitochondria and coordinate microtubule- and actin-based motor activities. Here, we...

...Gina A. Monzon; Lara Scharrel; Ashwin DSouza; Verena Henrichs; Ludger Santen; Stefan Diez ABSTRACT The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor...

...Safia Omer; Katia Brock; John Beckford; Wei-Lih Lee ABSTRACT The current model for spindle positioning requires attachment of the microtubule (MT) motor cytoplasmic dynein to the cell cortex, where it generates pulling force on astral MTs to effect spindle displacement. How dynein is anchored...

... structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions. Cilia Cryo-electron microscopy Dynein Flagella...

..., we test the model in RPE cells by imaging melanosomes associated with labeled actin filaments and microtubules, and by investigating the roles of different motor proteins. We found that a deficiency in cytoplasmic dynein phenocopies the lack of myosin-7a, in that melanosomes undergo fewer of the slow...

...Riya Keshri; Ashwathi Rajeevan; Sachin Kotak ABSTRACT Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches...

... at the immunological synapse. Furthermore, DISC1, Girdin and dynein are bound in a complex. Although this complex initially forms as a central patch at the synapse, it relocates to a peripheral ring corresponding to the peripheral supramolecular activation cluster (pSMAC). In the absence of DISC1, the classic actin...