... from MDA-MB-231 TNBC cells cultured on 2 kPa gels, which mimic the pre-metastatic stroma, drives efficient MSC chemotaxis and induces stable differentiation of MSC-derived CAFs in a TGFβ (TGFB1)- and contractility-dependent manner. In addition to enhancing cancer cell proliferation, MSC-derived CAFs...

... role in the choice between distinct forms of mitotic arrest – reversible quiescence and terminal differentiation, which play distinct roles in tissue homeostasis. We outline evidence implicating the centrosome–basal body switch in stem cell function, including how the cilium–centrosome complex...

...Erez Cohen; Craig Johnson; Catherine J. Redmond; Raji R. Nair; Pierre A. Coulombe ABSTRACT A large group of keratin genes ( n =54 in the human genome) code for intermediate filament (IF)-forming proteins and show differential regulation in epithelial cells and tissues. Keratin expression can...

... organizer CTCF in regulating expression of the HOXA gene locus during differentiation. ChIP sequencing revealed a significant overlap between Nup93 and CTCF peaks. Interestingly, Nup93 and CTCF are associated with the 3′ and 5′ HOXA genes, respectively. Depletions of Nup93 and CTCF antagonistically modulate...

... chondrocyte quiescence and immature chondrocyte state through reducing the amount of H3K9me3-marked heterochromatin. Chondrocyte Differentiation Heterochromatin Hydrostatic pressure Mechanotransduction Nucleus Replicative stress Helsinki Institute of Life Science Wihuri...

... of histone variants and gene position changes with respect to the nuclear periphery. Here, using high-resolution microscopy and quantitative image analysis, we surveyed a panel of histone modifications for changes in nuclear peripheral enrichment during differentiation of human embryonic stem cells...

... mechanistic evidence indicating that ubiquitin carboxyl-terminal hydrolase 8 (USP8) modulates BDNF- and TrkB-dependent neuronal differentiation. USP8 binds to the C-terminus of TrkB using its microtubule-interacting domain (MIT). Immunopurified USP8 deubiquitylates TrkB in vitro , whereas knockdown of USP8...

... in lineage specification, proliferative potential, differentiation, cell death and survival, DNA damage response and metabolism. Furthermore, p63 is linked to human disease states including cancer. p63 is critical to many aspects of cell signaling, and in this Cell science at a glance article...

...Shinji Kondo; Hidemasa Kato; Yutaka Suzuki; Toyoyuki Takada; Masamitsu Eitoku; Toshihiko Shiroishi; Narufumi Suganuma; Sumio Sugano; Hidenori Kiyosawa ABSTRACT We developed an in vitro system to differentiate embryonic stem cells (ESCs) derived from reciprocally crossed F1 hybrid mice into neurons...

.... In line with this, CXCL2 overexpression attenuated proliferation, as well as differentiation, of osteoblasts in vitro . By contrast, CXCL2 downregulation promoted osteoblast expansion and differentiation. Mechanistically, CXCL2 inhibits the ERK1/2 (MAPK3/1) signaling pathway in osteoblasts. Activation...

... , in cultured Schwann cells and find that tau is present in these cells. Gene silencing of Mapt promoted Schwann cell proliferation and inhibited Schwann cell migration and differentiation. In vivo application of Mapt siRNA suppressed the migration of Schwann cells after sciatic nerve injury. Consistent...

... structural variations that result in a remarkable repertoire of diverse compounds. This diversity of GSLs is exploited during embryogenesis, when different GSLs are produced at specific developmental stages and along several differentiation trajectories. Importantly, plasma membrane receptors interact...

... regulation in neuroendocrine differentiated cells. This point mutation altered the promoter's regulatory mechanism by discrete changes in transcription factor TR occupancy and altered enrichment of the repressive chromatin modification of histone-3-lysine-9-trimethyl (H3K9Me3). Insights relating...

...Julie Perroud; Laurent Bernheim; Maud Frieden; Stephane Koenig ABSTRACT Ca 2+ signaling plays a key role during human myoblast differentiation. Among Ca 2+ -sensitive pathways, calcineurin is essential for myoblast differentiation and muscle regeneration. Nuclear factor of activated T-cell (NFAT...

...Julia Lindqvist; Elin Torvaldson; Josef Gullmets; Henok Karvonen; Andras Nagy; Pekka Taimen; John E. Eriksson ABSTRACT Nestin, a member of the cytoskeletal family of intermediate filaments, regulates the onset of myogenic differentiation through bidirectional signaling with the kinase Cdk5. Here...

...-dependent apoptosis only cell differentiation taken to the extreme?   FASEB J.   21 , 8 - 17 . 10.1096/fj.06-5912hyp Fernando , P. , Kelly , J. F. , Balazsi , K. , Slack , R. S. and Megeney , L. A. ( 2002 ). Caspase 3 activity is required for skeletal muscle differentiation...

...Marta N. Shahbazi; Daniel Peña-Jimenez; Francesca Antonucci; Matthias Drosten; Mirna Perez-Moreno ABSTRACT Epidermal homeostasis is tightly controlled by a balancing act of self-renewal or terminal differentiation of proliferating basal keratinocytes. An increase in DNA content as a consequence...

...Ksenia G. Kolobynina; Valeria V. Solovyova; Konstantin Levay; Albert A. Rizvanov; Vladlen Z. Slepak ABSTRACT Tescalcin (TESC, also known as calcineurin-homologous protein 3, CHP3) is a 24-kDa EF-hand Ca 2+ -binding protein that has recently emerged as a regulator of cell differentiation and growth...

...Christine Hofstetter; Justyna M. Kampka; Sascha Huppertz; Heike Weber; Andreas Schlosser; Albrecht M. Müller; Matthias Becker ABSTRACT Pluripotent embryonic stem cells (ESCs) are characterised by their capacity to self-renew indefinitely while maintaining the potential to differentiate into all...

... cells, whereas, during megakaryocytic differentiation, declining Gfi1b levels facilitate a robust induction of Rgs18. Concordantly, alterations in Rgs18 expression produce disparate outcomes by augmenting megakaryocytic and potently suppressing erythroid differentiation and vice versa. These phenotypes...