..., ablation of ET-20 lncRNA effectively blocks Tnc expression and with it EMT. Mechanistically, ET-20 interacts with desmosomal proteins, thereby impairing epithelial desmosomes and promoting EMT. A short transcript variant of ET-20 is shown to be upregulated in invasive human breast cancer cell lines, where...

...Lisa Müller; Katrin Rietscher; René Keil; Marvin Neuholz; Mechthild Hatzfeld ABSTRACT Desmosome remodeling is crucial for epidermal regeneration, differentiation and wound healing. It is mediated by adapting the composition, and by post-translational modifications, of constituent proteins. We have...

...Katrin Rietscher; René Keil; Annemarie Jordan; Mechthild Hatzfeld ABSTRACT Desmosomes are essential for strong intercellular adhesion and are abundant in tissues exposed to mechanical strain. At the same time, desmosomes need to be dynamic to allow for remodeling of epithelia during differentiation...

... mutations promulgate a mechanical role for this important cytoskeletal component. In stratified epithelia, keratin filaments form prominent radial spokes that are focused onto cell-cell contact sites, i.e. the desmosomes. In this Hypothesis, we draw attention to a subset of keratin filaments...

...Andrew R. Harris; Alicia Daeden; Guillaume T. Charras ABSTRACT Adherens junctions and desmosomes integrate the cytoskeletons of adjacent cells into a mechanical syncitium. In doing so, intercellular junctions endow tissues with the strength needed to withstand the mechanical stresses encountered...

... cadherin- and integrin-based adhesion structures and hence epidermal function. In this study, we show that a member of this family, Rnd3, regulates desmosomal cell–cell adhesion in that loss of Rnd3 expression leads to an increase in desmosomes at sites of cell–cell adhesion and altered colony morphology...

... epidermal keratins K5/K14, and electron microscopy revealed total absence of IFs in the suprabasal epidermis. Although plakoglobin was unchanged, the expression of the desmosomal proteins desmoplakin, desmocollin 1 and desmoglein 1 were altered and suprabasal desmosomes were smaller in Krt1 −/− ; Krt10...

... finding in our study of teeth in Perp -null mice was the separation and ectopic displacement of the ameloblasts from the SI at the secretory and transitional stages of amelogenesis. This observation is consistent with the reduced number and size of desmosomes at the ameloblast–SI interface in the teeth...

...Lisa M. Sevilla; Rachida Nachat; Karen R. Groot; Fiona M. Watt Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin overexpression in human...

...Jiangli Chen; Zhining Den; Peter J. Koch Desmocollin 3 (DSC3) belongs to a subfamily of cadherins and is a major component of desmosomes in keratinocytes of stratified epithelia, such as the epidermis. Based on its amino acid sequence homology to classical cadherins, such as E-cadherin, it has been...

... at the plasma membrane. The results suggest that maintenance of desmosomes and hemidesmosomes may require some tension, normally mediated by keratin attachments. 9 7 2004 © The Company of Biologists Limited 2004 2004 * Author for correspondence (e-mail: e.b.lane@dundee.ac.uk...

... of the ridges or above the dermal papillae. We found that in populations of palm keratinocytes, selected by their ability to adhere rapidly to type IV collagen, there were significantly reduced levels of desmoplakin and other major desmosome proteins. We then showed that a low desmoglein 3 (Dsg3) expression...

...Andrew P. South; Hong Wan; Michael G. Stone; Patricia J. C. Dopping-Hepenstal; Patricia E. Purkis; John F. Marshall; Irene M. Leigh; Robin A. J. Eady; Ian R. Hart; John A. McGrath Ablation of the desmosomal plaque component plakophilin 1 underlies the autosomal recessive genodermatosis, skin...

..., a 680 kDa desmosomal plaque protein that is well characterized in bovine muzzle epidermis. A cDNA clone (DY6, 3693 bp) was isolated by immunoscreening a mouse keratinocyte expression library with 33A-3D, and it was confirmed that DY6 has a partial coding sequence for desmoyokin. DY6 consists of highly...

...Joachim Arnemann; Kate H. Sullivan; Anthony I. Magee; Ian A. King; Roger S. Buxton ABSTRACT Desmosomal junctions are abundant in epidermis and contain two classes of transmembrane glycoprotein, the desmocollins and the desmogleins, which are members of the cadherin superfamily of Ca 2+ -dependent...

...P. Bogner; P. Skehan; S. Kenney; E. Sainz; M.A. Akeson; S. J. Friedman ABSTRACT Short-chain monocarboxylic acids (MCAs) selectively protect desmosomal junctions of MDCK cells from disruption by chelating agents and low calcium medium. This effect occurs in the millimolar concentration range...

...Katrina T. Trevor; Lara S. Steben ABSTRACT In diverse epithelia, cytoskeletal keratin intermediate filaments (IFs) associate with the cytoplasmic face of intercelluar junctional desmosomes. The processes underlying desmosome formation and keratin IF interactions remain unclear. We have examined F9...

...D. L. Mattey; G. Burdge; D. R. Garrod ABSTRACT The development or maturation of intercellular adhesions following their initiation has received very little attention even though this is an area of significance for a variety of in vivo processes. Using Ca 2+ -induced desmosome formation in MDCK...

...J. L. Holton; T. P. Kenny; P. K. Legan; J. E. Collins; J. N. Keen; R. Sharma; D. R. Garrod ABSTRACT The N-terminal sequence of a mixture of desmosomal glycoproteins 2 and 3 (dg2/3, desmocollins) from bovine nasal epidermis, prepared by electro-elution from polyacrylamide gels, was determined...

...E. P. Parrish; J. E. Marston; D. L. Mattey; H. R. Measures; R. Venning; D. R. Garrod ABSTRACT Metabolic labelling with [ 35 S]methionine and immu-noprecipitation with specific antibodies to bovine desmosomal glycoproteins 2 and 3 (dg2 and dg3: desmocollins) reveals a triplet of polypeptides of M r...