... induced misplacement of basal bodies, a defect in the furrow or fold generation, and eventually cell death. We hypothesize that TFK1 is a basal body positioning-specific actor and a key regulator of cytokinesis in the bloodstream form Trypanosoma brucei . * Author for correspondence ( ddacheux...

...Günther Gerisch; Jana Prassler; Mary Ecke ABSTRACT In multi-nucleate cells of Dictyostelium , cytokinesis is performed by unilateral cleavage furrows that ingress the large cells from their border. We use a septase (sepA)-null mutant with delayed cytokinesis to show that in anaphase a pattern...

... membrane-localized fission yeast polycystin homolog Pkd2p is an essential protein required for cytokinesis; however, its role remains unclear. Here, we isolated a novel temperature-sensitive pkd2 mutant, pkd2-B42 . Among the strong growth defects of this mutant, the most striking was that many mutant cells...

...Imge Ozugergin; Karina Mastronardi; Chris Law; Alisa Piekny ABSTRACT Cytokinesis occurs at the end of mitosis as a result of the ingression of a contractile ring that cleaves the daughter cells. The core machinery regulating this crucial process is conserved among metazoans. Multiple pathways...

...Julie Rich-Robinson; Afton Russell; Eleanor Mancini; Maitreyi Das ABSTRACT In fission yeast, polarized cell growth stops during division and resumes after cytokinesis completes and cells separate. It is unclear how growth reactivation is timed to occur immediately after cell separation. We...

...Alaina H. Willet; Maya G. Igarashi; Jun-Song Chen; Rahul Bhattacharjee; Liping Ren; Sierra N. Cullati; Zachary C. Elmore; Rachel H. Roberts-Galbraith; Alyssa E. Johnson; Janel R. Beckley; Kathleen L. Gould ABSTRACT The F-BAR protein Imp2 is an important contributor to cytokinesis in the fission...

...Nicholas V. G. Iannantuono; Gregory Emery ABSTRACT Cytokinesis occurs at the end of mitosis/meiosis wherein the cytoplasms of daughter cells are separated. Before abscission, an intercellular bridge containing the remaining furrowing machinery, mitotic spindle and actin cytoskeleton connects...

... and is required for cytokinesis. The association between KIF1BP and CITK can be influenced by CITK activity, and the two proteins may antagonize each other for their midbody localization. KIF1BP knockdown decreases microtubule stability, increases KIF23 midbody levels and impairs midbody localization of KIF14...

...: A discussion of the roles of the spindle midzone, a microtubule-containing structure that connects the two half-spindles during mitosis, contributes to chromosome segregation and specifies the location of cytokinetic furrowing. Midzone Cytokinesis Anaphase Spindle elongation Microtubule...

...Mathieu C. Husser; Noha Skaik; Vincent J. J. Martin; Alisa Piekny ABSTRACT Cytokinesis is the process that separates a cell into two daughter cells at the end of mitosis. Most of our knowledge of cytokinesis comes from overexpression studies, which affects our interpretation of protein function...

...Patrick Partscht; Borhan Uddin; Elmar Schiebel ABSTRACT The budding yeast phosphatase Cdc14 has a central role in mitotic exit and cytokinesis. Puzzlingly, a uniform picture for the three human CDC14 paralogues CDC14A, CDC14B and CDC14C in cell cycle control has not emerged to date. Redundant...

...Stefano Sechi; Anna Frappaolo; Angela Karimpour-Ghahnavieh; Roberta Fraschini; Maria Grazia Giansanti ABSTRACT In animal cell cytokinesis, interaction of non-muscle myosin II (NMII) with F-actin provides the dominant force for pinching the mother cell into two daughters. Here we demonstrate...

...Destiny J. Davis; Minmin Wang; Iben Sørensen; Jocelyn K. C. Rose; David S. Domozych; Georgia Drakakaki ABSTRACT Cytokinesis in land plants involves the formation of a cell plate that develops into the new cell wall. Callose, a β-1,3 glucan, accumulates at later stages of cell plate development...

... microtubule bundles bridging daughter cells are severed. Here, we present a novel abscission mechanism. We identified chromokinesin KIF4A, which is adjacent to the midbody during cytokinesis, as being required for efficient abscission. KIF4A is regulated by post-translational modifications. We evaluated...

... division. FtsZ Bacteria Bacterial cell biology Cell division Cytokinesis Peptidoglycan When observed by time-lapse microscopy, bacterial cell division looks very simple. Start with one bacterium; end up with two. As with most biological processes, however, things are more complicated...

... the same GTPase. Using the GEF localization pattern during cytokinesis as a paradigm, we report a novel interplay between Gef1 and Scd1 that spatially modulates Cdc42. We find that Gef1 promotes Scd1 localization to the division site during cytokinesis through recruitment of the scaffold protein Scd2, via...

... forces during cytokinesis is not well understood. Here we show that, under mechanical stress, cortical actomyosin flow can switch polarization during cytokinesis in the C. elegans embryo. In unstressed embryos, longitudinal cortical flow contributes to contractile ring formation, while rotational...

...Aryeh Babkoff; Einav Cohen-Kfir; Hananel Aharon; Daniel Ronen; Michael Rosenberg; Reuven Wiener; Shoshana Ravid ABSTRACT An acto-myosin contractile ring, which forms after anaphase onset and is highly regulated in time and space, mediates cytokinesis, the final step of mitosis. The chromosomal...

...Harsh Kumar; Kumari Pushpa; Amrita Kumari; Kuldeep Verma; Rajaiah Pergu; Sivaram V. S. Mylavarapu ABSTRACT Cytokinesis is the final step of cell division following chromosome segregation that generates two daughter cells. The conserved exocyst complex is required for scission of the intercellular...

... also regulates cytokinesis initiation. How TbPLK is targeted to its subcellular locations remains poorly understood. Here we report the trypanosome-specific protein BOH1 that cooperates with TbPLK to regulate flagellum inheritance and cytokinesis initiation in the procyclic form of T. brucei . BOH1...