...Meng-Meng Shan; Ping-Shuang Lu; Yuan-Jing Zou; Kun-Huan Zhang; Jing-Cai Liu; Jia-Qian Ju; Shao-Chen Sun The meiotic spindle organization and cytokinesis are important for mammalian oocyte maturation. CLIP-associating protein (CLASP) 1 is a member of microtubule plus-end binding proteins, which...

...Ondrej Maxian; Katrina M. Longhini; Michael Glotzer ABSTRACT Cell polarization and cytokinesis are fundamental processes in organismal development. In the Caenorhabditis elegans model system, both processes are partially driven by local inhibition of contractility at the cell poles. This inhibition...

...Alaina H. Willet; Joshua S. Park; Chloe E. Snider; Jingdian Jamie Huang; Jun-Song Chen; Kathleen L. Gould ABSTRACT Cytokinesis is the final stage of the cell cycle that results in the physical separation of daughter cells. To accomplish cytokinesis, many organisms build an actin- and myosin-based...

... in these events. However, mechanistical understanding of mitosis and cytokinesis in dinoflagellates remains rudimentary. Using an optimized immunofluorescence protocol, we analysed changes in microtubule organization occurring during the mitotic cycle of the toxic dinoflagellate Ostreopsis cf. ovata. We find...

...Özge Uysal Özdemir; Andrea Krapp; Bastien Mangeat; Marc Spaltenstein; Viesturs Simanis ABSTRACT The septation initiation network (SIN) is a conserved signal transduction network, which is important for cytokinesis in Schizosaccharomyces pombe . The SIN component Etd1p is required for association...

... provided that the original work is properly attributed. Summary: Fission yeast Pik1 localizes exclusively to the trans-Golgi independently of Ncs1, where it contributes to PI4P but not PI(4,5)P 2 synthesis. Pik1 does not affect cytokinesis. PI4P PI(4,5)P 2 Lipid kinase Pik1 Fission...

...James Glover; Edward J. Scourfield; Leandro N. Ventimiglia; Xiaoping Yang; Steven Lynham; Monica Agromayor; Juan Martin-Serrano ABSTRACT Abscission is the final stage of cytokinesis whereby the midbody, a thin intercellular bridge, is resolved to separate the daughter cells. Cytokinetic abscission...

.... , Sinha , D. , Poddar , A. , Morris, , B. and Chen , Q. (2019). Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis . Mol. Biol. Cell 30 , 1791 - 1804 . 10.1091/mbc.E18-04-0270 Mulholland , J. , Konopka...

...Udo N. Onwubiko; Dhanya Kalathil; Emma Koory; Sahara Pokharel; Hayden Roberts; Ahmad Mitoubsi; Maitreyi Das ABSTRACT During cytokinesis, a series of coordinated events partition a dividing cell. Accurate regulation of cytokinesis is essential for proliferation and genome integrity. In fission yeast...

... cell surface and translocate to the cleavage furrow during cytokinesis. The localization of PCDH7 depends on the palmitoylation activity of ZDHHC5. Silencing PCDH7 increases the percentage of multinucleated cells and the duration of mitosis. Loss of PCDH7 expression correlates with reduced levels...

... polyploidization in a mammalian ancestor. Cytokinesis Evolution Megakaryocytes Platelets Polyploidy University of Cambridge http://dx.doi.org/10.13039/501100000735 Evolution through natural selection as described by Darwin is the bedrock of biodiversity (Darwin, 1859). Among...

... filaments, required for cytokinesis by many organisms. In fission yeast cofilin mutants, contractile rings suffer bridging instabilities in which segments of the ring peel away from the plasma membrane, forming straight bridges whose ends remain attached to the membrane. The origin of bridging instability...

... induced misplacement of basal bodies, a defect in the furrow or fold generation, and eventually cell death. We hypothesize that TFK1 is a basal body positioning-specific actor and a key regulator of cytokinesis in the bloodstream form Trypanosoma brucei . The pGAD-T7 vectors were transformed...

...Günther Gerisch; Jana Prassler; Mary Ecke ABSTRACT In multi-nucleate cells of Dictyostelium , cytokinesis is performed by unilateral cleavage furrows that ingress the large cells from their border. We use a septase (sepA)-null mutant with delayed cytokinesis to show that in anaphase a pattern...

... membrane-localized fission yeast polycystin homolog Pkd2p is an essential protein required for cytokinesis; however, its role remains unclear. Here, we isolated a novel temperature-sensitive pkd2 mutant, pkd2-B42 . Among the strong growth defects of this mutant, the most striking was that many mutant cells...

...Imge Ozugergin; Karina Mastronardi; Chris Law; Alisa Piekny ABSTRACT Cytokinesis occurs at the end of mitosis as a result of the ingression of a contractile ring that cleaves the daughter cells. The core machinery regulating this crucial process is conserved among metazoans. Multiple pathways...

...Julie Rich-Robinson; Afton Russell; Eleanor Mancini; Maitreyi Das ABSTRACT In fission yeast, polarized cell growth stops during division and resumes after cytokinesis completes and cells separate. It is unclear how growth reactivation is timed to occur immediately after cell separation. We...

...Alaina H. Willet; Maya G. Igarashi; Jun-Song Chen; Rahul Bhattacharjee; Liping Ren; Sierra N. Cullati; Zachary C. Elmore; Rachel H. Roberts-Galbraith; Alyssa E. Johnson; Janel R. Beckley; Kathleen L. Gould ABSTRACT The F-BAR protein Imp2 is an important contributor to cytokinesis in the fission...

...Nicholas V. G. Iannantuono; Gregory Emery ABSTRACT Cytokinesis occurs at the end of mitosis/meiosis wherein the cytoplasms of daughter cells are separated. Before abscission, an intercellular bridge containing the remaining furrowing machinery, mitotic spindle and actin cytoskeleton connects...

... and is required for cytokinesis. The association between KIF1BP and CITK can be influenced by CITK activity, and the two proteins may antagonize each other for their midbody localization. KIF1BP knockdown decreases microtubule stability, increases KIF23 midbody levels and impairs midbody localization of KIF14...