... of vimentin and cytokeratin 5/14 filaments through plectin's intermediate filament-binding domain . J. Cell Sci. 113 , 483 - 491 . Strnad P. , Windoffer R. , Leube R. E. ( 2002 ). Induction of rapid and reversible cytokeratin filament network remodeling by inhibition of tyrosine...

... of cytoskeleton in mRNA anchoring was tested by treating oocytes with inhibitors that selectively disrupted actin microfilaments and cytokeratin filaments. Treatment of oocytes with cytochalasin B caused clumping of Vg1 and Xwnt-11 as revealed by in situ hybridization of the IVC, but did not cause their release...

... a remarkable dual location: they occur in the nuclei of cells and, in epithelial cells, at the plasma membrane within the desmosomal plaques. We have shown by solid phasebinding assays that both PKP1a and PKP2a bind to intermediate filament (IF) proteins, in particular to cytokeratins (CKs) from epidermal...

... of cytokeratin and F-actin during autophagic cell death in the human mammary carcinoma cell line MCF-7 because recent studies suggest that an intact cytoskeleton is necessary for autophagocytosis. Programmed cell death was induced by 10 −6 M tamoxifen. For quantitative light microscopic analysis, autophagic...

... of plectin repeat 5-binding to IF proteins of different types. Quantitative Eu 3+ -based overlay assays showed that plectin’s repeat 5 domain bound to type III IF proteins (vimentin) with preference over type I and II cytokeratins 5 and 14. The ability of both types of IF proteins to self-assemble...

...Reinhard Windoffer; Rudolf E. Leube ABSTRACT To monitor the desmosome-anchored cytokeratin network in living cells fusion protein HK13-EGFP consisting of human cytokeratin 13 and the enhanced green fluorescent protein was stably expressed in vulvar carcinoma-derived A-431 cells. It is shown...

... to a luminal phenotype. Under these growth-restrictive conditions, immunocytochemical analysis shows that NRP-152 cells acquire the luminal markers Z0-1 (a tight-junction associated protein), occludin (integral tight-junction protein), and cytokeratin 18, and lose the basal markers cytokeratins 5 and 14. Total...

...Steffen Goletz; Franz-Georg Hanisch; Uwe Karsten ABSTRACT We report on a novel posttranslational modification of cytoplasmic proteins. Presented evidences suggest that cytokeratins are bound in vitro by mammalian galectin-3 and the galectins from the sponge Geodia cydonium via their type II...

...Andreas Eger; Andreas Stockinger; Gerhard Wiche; Roland Foisner ABSTRACT The intermediate filament-binding protein plectin and cytokeratin were localised at the cellular periphery of fully polarised Madin-Darby canine kidney (MDCK) cells, whereas vimentin was primarily found in a perinuclear...

... protein was identified in the plasma membrane fraction of rat hepatocytes. The protein had an apparent mass of 59 kDa, was insoluble in a spectrum of detergents, and was identical to cytokeratin 8 (CK 8) as determined by sequence analysis of nine amino acids at the N terminus of two cyanogen bromide...

... the apical distribution of a 53 kDa cytokeratin. Furthermore, this cytokeratin co-purified with biotinylated apical plasma membrane proteins in high density complexes. Differential biotinylation of the basolateral domain showed that the 53 kDa protein is mainly attached to the apical membrane, although...

... organiz-ation of the cytokeratin network that correlate with an increased phosphorylation of cytokeratin molecules, whereas microtubules, microfilaments and vimentin remain mainly unaffected. Indirect immunofluorescence studies show that the cytokeratin network is redistributed from the cell periphery...

... on those of the intermediate filaments of the cytokeratin type. We show here that in PtK 1 cells various prosomal antigens also reside to a variable extent on intermediate filaments subnetworks of the vimentin type. In proliferating human fibroblasts the prosome and vimentin networks were found to coincide...

...Helen L. Robey; Paul S. Hiscott; Ian Grierson ABSTRACT The expression of cytokeratins 18 and 19 by human retinal pigment epithelial cells (HRPE) has been suspected of being associated with HRPE proliferation. We have investigated the involvement of these cytokeratin subtypes in the proliferative...

... as Xenopus vimentin. Direct microsequencing of the M r 57,000 protein shows near identity of three tryptic fragments with regions of the predicted amino acid sequence of XCK1(8), a basic cytokeratin whose mRNA is known to be expressed in Xenopus oocytes. We have raised an antibody, CK7, against a fusion...

... and entangled cytoplasmic microtubule network, a perinuclear cage of cytokeratin fibers, and a diffuse distribution of F-actin were revealed. Short-term stimulation of these cells by TRH induces a first early phase of PRL release (0-2 min), concomitant with a rarefaction of cytoplasmic PRL-containing granules...

...; predominantly in astrocytes), several different neurofilament proteins (in most neurons), peripherin (in certain neuronal cells), at least four different lamins (structural components of the nuclear lamina), and a whole panopticum of cytokeratins (present in almost all epithelial cells). A given tissue...

...Nicol M. Mckechnle; Mike Boulton; Helen L. Robey; Felicity J. Savage; Ian Grierson ABSTRACT The cytoskeletal elements of normal (in situ) and cultured human retinal pigment epithelium (RPE) were studied by a variety of immunocytochemical techniques. Primary antibodies to vimentin and cytokeratins...

... that these human lung adenocarcinoma, classic SCLC and variant SCLC cell lines can be differentiated on the basis of their pattern of IFP. The main conclusions from this study can be summarized as follows. (1) The two adenocarcinoma cell lines contain cytokeratins 7, 8, 18, and sometimes 19, next to vimentin...

... , B. ( 1981 ). Diversity of cytokeratins: differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues. J . molec. Biol . 153 , 933 – 959 . 10.1016/0022-2836(81)90460-5 Franke , W. W. , Schmid , E. , Freudenstein , C. , Appelhans , B...