.... Subbundle adhesion was lost in the presence of cytochalasin, indicating that continuous actin polymerization is required. Thus, these polarized actin filament bundles are dynamic structures that require continuous maintenance owing to protein and actin filament turnover. We propose that after cell...

... significantly decreased cell length on both substrates, whereas cytochalasin D increased the length. We concluded that fibroblasts have an intracellular mechanism maintaining a relatively constant average cell length. This mechanism may involve the dynamic balance of centripetal and centrifugal forces developed...

... (10 7 to 10 5 M) of phorbol myristate acetate but not of chemotactic peptide induced down-regulation of surface CD18. Cytochalasin D (10 4 M) stimulated CD18 expression even though it inhibited shape changes. The surface distribution of CD18 determined by light microscopy was uniform in unstimulated...

... immunofluorescence. The cytoskeletal inhibitors nocodazole, cytochalasin D and 2,3-butanedione monoxime were used to examine the roles of actin and myosin in ascus development. Microfilament and microtubule arrays are interdependant; disruption of one network results in abnormalities in the other. Both...

...E. P. Eleftheriou; B. A. Palevitz ABSTRACT The relationship between microfilaments (Mfs) and microtubules (Mts) in the organization of the preprophase band (PPB) was investigated in Allium root tip cells subjected to treatment with cytochalasin D (CD). Mts and Mfs were visualized by indirect...

... in PPB organization and/or function remains unclear. The present study employed fluorescence and pharmacological methods in symmetrically and asymmetrically dividing epidermal cells of Allium to probe this problem. Our results show that PPBs in cells treated with 2–200 μ M cytochalasin D (CD) are still...

...Richard E. Williamson; Ursula A. Hurley ABSTRACT Cytochalasin is known to inhibit cytoplasmic streaming rapidly in characean cells without disassembling their actin bundles. Lower cytochalasin concentrations than those needed for streaming inhibition are now shown to disrupt bundle assembly...

...Janet Tannenbaum; Armand F. Miranda ABSTRACT Treatment of human muscle myotube cultures with 2 μ M-cytochalasin D (CD) for 6 h stimulated synthesis of both the (muscle-specific) a-actin and the (non-muscle) β and γ-actins usually expressed by these cells. In non-muscle (HEp-2) cell cultures, CD...