... that surface articular chondrocytes (which occupy the uppermost 10– 15% of the tissue depth) from normal human cartilage initiate de novo synthesis of both type X collagen and alkaline phosphatase when maintained in suspension culture. REFERENCES Adams , S.H. , Pallante , K.M. , Nui , Z...

..., immunoprecipitation and hybridization with cDNA probes. This study was carried out on undisturbed and regenerating scales in situ and in organ and cell cultures from regenerating scales. The hyposquamal scleroblasts forming a pseudoepithelium show an apico-basal polarization and synthesize thick collagen fibrils (100...

... culture either as a low density monolayer or as a suspension over agarose revealed distinct morphological and synthetic behaviour in the two populations. Whilst in monolayer these morphological differences disappeared with time in culture, over agarose they remained. Surface zone cells formed two types...

... with other components to be microfilaments. 29 01 1987 12 05 1987 © 1987 by Company of Biologists 1987 corneal epithelium cytoskeleton culture NBD-phallacidin F-actin epithelial spreading Epithelial cells can spread quickly along the surface of a wound bed in vivo when...

... for correspondence. 22 04 1986 26 06 1986 © 1986 by Company of Biologists 1986 epithelial lung cells transformation culture The regulation and/or the maintenance of cell differentiation operated by the supporting extracellular matrix or the basement membrane might be significant...

... containing the junction between the secretory coil and the collecting duct. Primary cultures were obtained from explants of both tissues in medium RPMI-1640 or Williams E supplemented with foetal calf serum, insulin, transferrin, epidermal growth factor and hydrocortisone. The cells in the initial coil...

... unpigmented and pigmented progeny. Thus there is a separate cell type, or premelanocyte, which can differentiate spontaneously and stably in culture; this cell type includes both unpigmented and faintly pigmented cells. Usefully, most premelanocytes are viable after frozen storage, unlike well-pigmented...