... for spatially and temporally ordered contraction in adult cardiomyocytes. However, the mechanism(s) underlying the control of cardiomyocyte alignment remains elusive. Here, we report that SIRT1, the most conserved NAD + -dependent protein deacetylase highly expressed in perinatal heart, plays an important role...

... fibroblasts did not differ in in vitro assays of adhesion or migration; however, in 3D culture, periostin-knockout fibroblasts showed a significantly reduced ability to contract a collagen matrix, and adopted a dendritic phenotype. Recombinant periostin restored the defects in cell morphology and matrix...

... when RhoA is active. © 2010. 2010 Migration Contraction Phosphorylation Focal adhesion We thank the staff of the Second Core Lab, Department of Medical Research, National Taiwan University Hospital for technical support and R. H. Chen (Institute of Biological Chemistry, Academia...

... step in smooth muscle contraction. * Author for correspondence ( [email protected] ) 16 5 2010 © 2010. 2010 Airway smooth muscle Dystrophin Actin G-protein coupled receptors Cytoskeleton Contraction Smooth muscle in hollow organs is subject to cellular...

... regulators of contraction of the myoepithelial sheath. These are two of the three components of the troponin complex that is generally considered as a striated-muscle-specific regulator of actomyosin contraction. Here, we report identification of troponin I as the third component of the troponin complex...

.... Gerthoffer, W. T. ( 2005 ). Actin cytoskeletal dynamics in smooth muscle contraction. Can. J. Physiol. Pharmacol. 83 , 851 -856. Hall, D. H., Winfrey, V. P., Blaeuer, G., Hoffman, L. H., Furuta, T., Rose, K. L., Hobert, O. and Greenstein, D. ( 1999 ). Ultrastructural features of the adult...

...Lauren Mackenzie; H. Llewelyn Roderick; Michael J. Berridge; Stuart J. Conway; Martin D. Bootman We examined the regulation of calcium signalling in atrial cardiomyocytes during excitation-contraction coupling, and how changes in the distribution of calcium impacts on contractility. Under control...

... measurements show that a myosin-dependent viscoelastic force resists cell deformation. * Author for correspondence (e-mail: [email protected] ) 2 1 2003 © The Company of Biologists Limited 2003 2003 Myosin II Cell spreading Cell mechanics Traction force Contraction...

...Alisa J. Piekny; Paul E. Mains Rho-binding kinase and myosin phosphatase regulate the contraction of actomyosin filaments in non-muscle and smooth muscle cells. Previously, we described the role of C. elegans genes encoding Rho-binding kinase( let-502 ) and myosin phosphatase targeting subunit...

... protrusions of such cells was four-times higher than the flux into the elongating cylindrical protrusions of the cells treated with quinine alone. These results indicate that the force responsible for the quinine-induced protrusion is mainly due to contraction of the cell body, which requires normal myosin II...

...Mary C. Reedy ABSTRACT The long-standing swinging crossbridge or lever arm hypothesis for the motor action of myosin heads finds support in recent results from 3-D tomograms of insect flight muscle (IFM) fast frozen during active contraction and from both fluorescence polarization and X-ray...

... for correspondence (e-mail: [email protected] ) 18 11 1999 19 01 2000 © 2000 by Company of Biologists 2000 PAK Myosin II Actin Contraction Cdc42 The vascular system is lined by an intact monolayer of endothelial cells whose major function is to serve as a selective...

... that myosin IC mediates the purse-string-like contraction that closes phagosomes. The sequential processes of pseudopod extension and contraction can explain how macropinosomes and spacious phagosomes form without guidance from a particle surface. * Author for correspondence at the Department...

.... Immunofluorescence and in situ hybridization techniques revealed that contracting myocytes display well aligned myofibrils and a diffuse distribution of α-myosin heavy chain mRNA. Inhibition of contractile activity with the calcium channel blocker verapamil (10 μM) resulted in myofibril disassembly and a perinuclear...

...Onno Kranenburg; Mieke Poland; Martijn Gebbink; Lauran Oomen; Wouter H. Moolenaar ABSTRACT Addition of lysophosphatidic acid (LPA) to serum-deprived N1E-115 neuronal cells results in rapid f-actin assembly accompanied by neurite retraction and rounding of the cell body due to contraction...

... as contraction. Buffered EGTA medium supplemented with isotonic glycerol and with magnesium, at a level close to the reported internal cellular concentration, yields stable cytoskeletal cortices that retain their spherical shape. Cortices prepared this way contain actin, myosin, fascin and spectrin, components...

... conclude that the mini-titins of invertebrate muscles defined earlier by ultrastructural criteria are very likely to be twitchins, i.e. molecules necessary for normal muscle contraction. We discuss the molecular properties of the proteins in the light of the sequence established for twitchin...

...R. D. Butler; C. R. Mccrohan ABSTRACT Experiments were carried out to determine the role of the plasma membrane in the control of tentacle contraction in Heliophrya erhardi . Intracellular recordings gave membrane potentials between −20 and −30 mV. In a Ca 2+ -containing medium mechanical...