... cell surface and translocate to the cleavage furrow during cytokinesis. The localization of PCDH7 depends on the palmitoylation activity of ZDHHC5. Silencing PCDH7 increases the percentage of multinucleated cells and the duration of mitosis. Loss of PCDH7 expression correlates with reduced levels...

..., proteins that regulate cortical stability during anaphase and telophase have been understudied. Here, we describe a role for CLIC4 in regulating actin and actin regulators at the cortex and cytokinetic cleavage furrow during cytokinesis. We first describe CLIC4 as a new component of the cytokinetic...

.... 21 12 2015 19 7 2016 © 2016. Published by The Company of Biologists Ltd 2016 Summary: Disruption of the FASII fatty acid synthesis pathway in the apicoplast of Toxoplasma gondii leads to a defect in cleavage furrow formation, preventing completion of cytokinesis. Cell...

...Hasan Ucar; Kazunori Tachibana; Takeo Kishimoto Summary Maintenance of spindle attachment to the cortex and formation of the cleavage furrow around the protruded spindle are essential for polar body extrusion (PBE) during meiotic maturation of oocytes. Although spindle movement to the cortex has...

...Pier Paolo D'Avino The ingression of a cleavage furrow separates the two daughter cells at the end of cell division. In many organisms this furrow ingression is driven by the assembly and contraction of actomyosin filaments, forming a contractile ring. To achieve a successful cytokinesis...

... through its BRCT domain, whereas MKlp2–Aurora-B probably exerts a regulatory role in Ect2 central spindle targeting. Depletion of Ect2 impaired cleavage furrow formation and RhoA and Citron kinase failed to accumulate at the cleavage furrow. Ect2 displacement from the central spindle revealed...

... of the cleavage furrow, the molecules responsible for these processes have remained elusive. Recent studies have provided insights into the role of different microtubule structures and associated proteins in cleavage furrow positioning and ingression together with the signalling events that regulate the dynamics...

... of actin filaments and positioning of cell cleavage in grasshopper spermatocytes. * Author for correspondence (e-mail: [email protected] ) 24 11 2003 © The Company of Biologists Limited 2004 2004 Cytokinesis Microtubule Actin filament Cleavage furrow Spindle...

.... * Author for correspondence (e-mail: [email protected] ) 10 10 2002 © The Company of Biologists Limited 2003 2003 Cleavage furrow Cytokinesis D 2 O Exocytosis Fusion pore Microtubule Xenopus It is now well established that localized exocytosis occurs at or near...

... an intact cleavage furrow. However, we have found that cyk-3 mutant embryos are intrinsically defective in osmotic regulation and that the cytokinesis defects can be partially rescued by providing osmotic support. The cyk-3 gene has been identified and found to encode a ubiquitin C-terminal hydrolase...

...Masatoshi Eda; Shigenobu Yonemura; Takayuki Kato; Naoki Watanabe; Toshimasa Ishizaki; Pascal Madaule; Shuh Narumiya Citron-kinase (Citron-K) is a Rho effector working in cytokinesis. It is enriched in cleavage furrow, but how Rho mobilizes Citron-K remains unknown. Using anti-Citron antibody...

...Tatsuhiko Noguchi; Issei Mabuchi ABSTRACT We studied reorganization of actin-myosin cytoskeleton at the growing ends of the cleavage furrow of Xenopus eggs in order to understand how the contractile ring is formed during cytokinesis. Reorganization of F-actin structures during the furrow formation...

... of the equatorial cortex in 100% of cells. This concentration of myosin-II isozymes in the cleavage furrow is maintained until the daughter cells separate. The superimposition of these small spots concentrated in the cleavage furrow produces the intense, uniform staining observed in conventional micrographs...

...Issei Mabuchi ABSTRACT Cleavage furrow formation at the first cell division of sea urchin and sand dollar eggs was investigated in detail by fluorescence staining of actin filaments with rhodamine-phalloidin of either whole eggs or isolated egg cortices. Cortical actin filaments were clustered...

...Shigenobu Yonemura; Issei Mabuchi; Shoichiro Tsuktta ABSTRACT To develop a mass isolation procedure for the cleavage furrow from synchronized sea urchin eggs, we compared the stability of the cleavage furrow with that of the rest of the cortex (polar-region cortex) and the inner cytoplasm under...

... the cleavage furrow forms, it does so around the maximum circumference of the cell. During anaphase, a subpopulation of the INCENP antigen becomes localized to the cortex where the furrow will subsequently form. This occurs prior to any other evidence of furrowing. Thus, binding of the INCENPs to this region...