... of SMLM direct stochastic optical reconstruction microscopy (dSTORM) data to determine how the clathrin endocytosis inhibitors pitstop 2, dynasore and latrunculin A (LatA) alter the morphology of clathrin-coated pits. SuperResNET analysis of HeLa and Cos7 cells identified three classes of clathrin...

...Stéphane Vassilopoulos; Guillaume Montagnac ABSTRACT Clathrin assembles into honeycomb-like lattices at the plasma membrane but also on internal membranes, such as at the Golgi and tubular endosomes. Clathrin assemblies primarily regulate the intracellular trafficking of different cargoes...

... are packaged into coated and non-coated transport carriers derived from the trans Golgi and TGN. The cargoes of clathrin-coated vesicles are chiefly residents of endo-lysosomal organelles, while uncoated carriers ferry cargo to the cell surface. There are outstanding questions regarding the mechanisms...

... of the AP2 adaptor complex, a key player in clathrin-mediated endocytosis, is a highly organized process controlled by alpha- and gamma-adaptin-binding protein (AAGAB, also known as p34). In this study, we demonstrate that besides AP2, AAGAB also regulates the assembly of AP1, a cargo adaptor involved...

...Ellis L. Ryan; James Shelford; Teresa Massam-Wu; Richard Bayliss; Stephen J. Royle ABSTRACT A multiprotein complex containing TACC3, clathrin and other proteins has been implicated in mitotic spindle stability. To disrupt this complex in an anti-cancer context, we need to understand its composition...

...Francesco Baschieri; Dahiana Le Devedec; Samuel Tettarasar; Nadia Elkhatib; Guillaume Montagnac ABSTRACT Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results...

... a role for clathrin and/or endocytosis in the regulation of this pathway, but conflicting results exist and demand a deeper mechanistic understanding. We investigated the consequences of clathrin depletion on Wnt/β-catenin signaling in cell lines and found a pronounced reduction in β-catenin protein...

...Francesco Baschieri; Kseniia Porshneva; Guillaume Montagnac ABSTRACT Clathrin-mediated endocytosis is the main entry route for most cell surface receptors and their ligands. It is regulated by clathrin-coated structures that are endowed with the ability to cluster receptors and to locally bend...

... found in each of the complexes. Summary: Adaptor protein complexes select cargo for transport between subcellular compartments. Disease-causing mutations identified in each of the five complexes cause certain membrane proteins to be mislocalised. Adaptor protein complex Clathrin...

.... and Homann , U. ( 2012 ). Clathrin-independent endocytosis contributes to uptake of glucose into BY-2 protoplasts . Plant J.   70 , 578 - 584 . 10.1111/j.1365-313X.2011.04892.x Barberon , M. , Dubeaux , G. , Kolb , C. , Isono , E. , Zelazny , E. and Vert , G. ( 2014...

...Oxana M. Tsygankova; James H. Keen ABSTRACT Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show...

... matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, in human keratinocytes, αVβ5 is predominantly found in FCLs, and formation of the αVβ5-containing FCLs requires the presence...

...Volker Haucke; Michael M. Kozlov ABSTRACT Clathrin-mediated endocytosis is an essential cellular mechanism by which all eukaryotic cells regulate their plasma membrane composition to control processes ranging from cell signaling to adhesion, migration and morphogenesis. The formation of endocytic...

...Wataru Yamamoto; Suguru Wada; Makoto Nagano; Kaito Aoshima; Daria Elisabeth Siekhaus; Junko Y. Toshima; Jiro Toshima ABSTRACT Clathrin-mediated endocytosis requires the coordinated assembly of various endocytic proteins and lipids at the plasma membrane. Accumulating evidence demonstrates a crucial...

..., live-cell imaging and correlative spinning-disk microscopy and transmission electron microscopy assays we provide the first in-depth high-resolution characterisation of this process. We provide a model of compensatory endocytosis based on rapid clathrin- and dynamin-mediated retrieval. Inhibition...

...Paul T. Manna; Samson O. Obado; Cordula Boehm; Catarina Gadelha; Andrej Sali; Brian T. Chait; Michael P. Rout; Mark C. Field ABSTRACT Clathrin-mediated endocytosis (CME) is the most evolutionarily ancient endocytic mechanism known, and in many lineages the sole mechanism for internalisation...

... pathway that directly returns cargo from early endosomes to the cell surface. In contrast, NECAP2 does not regulate the clathrin-mediated endocytosis of these cargos, the degradation of EGFR or the recycling of transferrin along the slow, Rab11-dependent recycling pathway. We show that protein knockdown...

..., constituents of Glut4 storage vesicles (GSVs). We further found that CD2AP forms a complex with GGA2, a clathrin adaptor, which sorts Glut4 to GSVs, suggesting a role for CD2AP in this process. We also found that CD2AP forms a complex with clathrin and connects clathrin to actin in the perinuclear region...

... endocytosis of adhesion proteins has been shown to play a role in cell migration, we examined whether Cad11 endocytosis plays a role in Cad11-mediated migration. The mechanism by which Cad11 is internalized is unknown. Using a GST pulldown assay, we found that clathrin binds to the Cad11 cytoplasmic domain...

...Bum-Chan Park; Yang-In Yim; Xiaohong Zhao; Maciej B. Olszewski; Evan Eisenberg; Lois E. Greene ABSTRACT Cyclin-G-associated kinase (GAK), the ubiquitously expressed J-domain protein, is essential for the chaperoning and uncoating of clathrin that is mediated by Hsc70 (also known as HSPA8). Adjacent...