...Qisheng Zuo; Kai Jin; Man Wang; Yani Zhang; Guohong Chen; Bichun Li ABSTRACT The unique developmental characteristics of chicken primordial germ cells (PGCs) enable them to be used in recovery of endangered bird species, gene editing and the generation of transgenic birds, but the limited number...

...Miguel Weil; Michael D. Jacobson; Martin C. Raff ABSTRACT We show that mouse sperm die spontaneously within 1-2 days in culture and that treatment with either staurosporine (STS) and cycloheximide (CHX) or a peptide caspase inhibitor does not accelerate or delay the cell death. Chicken erythrocytes...

...Kenji Shimada; Masahiko Harata; Shigeki Mizuno ABSTRACT A hnRNP-free nuclear matrix prepared from chicken MSB-1 cells was used to raise monoclonal antibodies. The monoclonal antibodies 2H3 and 3B7 showed identical non-homogeneous immunofluorescence staining patterns of nuclei in MSB-1 cells...

... as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while three isoenzymes, cytosolic B-CK, sarcomeric mitochondrial CK (Mib-CK), and a variant...

...Christine L. Hatem; Nancy R. Gough; Douglas M. Fambrough ABSTRACT Lysosomal membranes are enriched in extensively glycosylated transmembrane proteins, LAMP-1 and LAMP-2. LAMP-1 proteins have been characterized from several mammalian species and from chickens, but no nonmammalian homologues of LAMP...

...M. Douaire; B. Belloir; J.-C. Guillemot; J.-M. Fraslin; P. Langlois; J. Mallard ABSTRACT Hepatocytes isolated from 9-week-old chickens were cultured in a serum-free, hormonally defined medium. Relative amounts of mRNAs coding for lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase, 9...

...Mathias G. Bergman; Edgar Wawra; MÅrten Winge ABSTRACT Chicken erythrocyte histone H5 has been suggested repeatedly to be a general suppressor of transcription and replication. Therefore, the biological functions of H5 were investigated and compared with those of Hl (Hla + Hlb) by microinjection...