... cells or fibroblasts, contain microtubule asters attached to the centrosome and the Golgi complex, whereas in many other differentiated cells, microtubules form linear arrays or meshworks anchored at membrane-bound organelles or the cell cortex. Over the past decade, new developments in cell culture...

.... Primary cilia Ciliopathy Actin Microtubule Centrosome Pericentriolar material Hyperosmotic stress Extracellular stress Japan Society for the Promotion of Science JP21K06172 Japan Science and Technology Agency http://dx.doi.org/10.13039/501100002241 Precursory...

... back into a cilium remain poorly understood. Here, we identify centrosomal protein of 170 kDa (CEP170) as a dynein-2-interacting protein in mammalian cells. We show that loss of CEP170 perturbs intraflagellar transport and hedgehog signalling, and alters the stability of dynein-2 holoenzyme complex...

... Al-Hakim , A. K. , Bashkurov , M. , Gingras , A. C. , Durocher , D. and Pelletier , L. ( 2012 ). Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture . Mol. Cell. Proteomics 11 , M111.014233 . 10.1074...

... and secretory domain at the interface with their target, the immune synapse (IS). During IS formation, CTLs acquire a transient polarity, marked by re-orientation of the centrosome and microtubule cytoskeleton toward the IS, thus directing the transport and delivery of the lytic granules to the target cell...

...Abrar Aljiboury; Heidi Hehnly ABSTRACT The centrosome is a non-membrane-bound organelle that is conserved across most animal cells and serves various functions throughout the cell cycle. In dividing cells, the centrosome is known as the spindle pole and nucleates a robust microtubule spindle...

... and mitosis. Pro-LC3B accumulated aberrantly at the centrosome with a concomitant increase in centrosomal proteins PCM1 and CEP131, which was rescued by exogenous ATG4B. The two-stage cell cycle defects following ATG4B and ATG4A loss have important therapeutic implications for PDAC. Fig. 3. ATG4B KO...

...Priti S. Atmakuru; Jyotsna Dhawan ABSTRACT The centrosome is an evolutionarily conserved, ancient organelle whose role in cell division was first described over a century ago. The structure and function of the centrosome as a microtubule-organizing center, and of its extracellular extension...

... and mitosis, calcineurin consistently associated with multiple centrosomal and/or ciliary proteins. These include POC5, which binds centrins in a Ca 2+ -dependent manner and is a component of the luminal scaffold that stabilizes centrioles. We show that POC5 contains a calcineurin substrate motif (PxIxIT type...

...Dandan Ma; Fulin Wang; Junlin Teng; Ning Huang; Jianguo Chen ABSTRACT Centrosomes are composed of centrioles surrounded by pericentriolar material. The two centrioles in G1 phase are distinguished by the localization of their appendages in the distal and subdistal regions; the centriole possessing...

... are restricted to proliferating crypt cells, including Lgr5+ stem cells, where the Mcc protein is distinctly associated with the centrosome. Upon intestinal cellular differentiation, Mcc is redeployed to the apical domain of polarized villus cells where non-centrosomal microtubule organizing centers (ncMTOCs...

... in ciliary cell biology. Basal body Centrosome Cilia Cytoplasmic transport Diffusion Motor proteins National Heart, Lung, and Blood Institute https://doi.org/10.13039/100000050 R01HL117164 Eunice Kennedy Shriver National Institute of Child Health and Human Development https...

... al., 2018 ), the beginning of S-phase was taken as the time of centrosome separation (‘CS’). Entry into mitosis was taken as the time of nuclear envelope breakdown (NEB). Living embryos were imaged at 21°C using a DeltaVision OMX V3 Blaze microscope (GE Healthcare Life Sciences, UK...

... of Thr3 phosphorylation on histone H3 (H3T3ph) does not alter borealin and SGO2 centromeric localization. Meiosis Centromere Centrosome Haspin H3T3ph Aurora B Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Ministerio de Ciencia e Innovación...

... of Biologists Ltd 2022 Highlighted Article: Characterization of new roles for retromer in C. elegans development and in the regulation of ciliogenesis in mammalian cells, suggesting a novel role for retromer in CP110 removal from the mother centriole. Centrosome Ciliogenesis CP110 Retromer...

...Morgan Le Roux-Bourdieu; Devashish Dwivedi; Daniela Harry; Patrick Meraldi ABSTRACT Centrioles are central structural elements of centrosomes and cilia. In human cells, daughter centrioles are assembled adjacent to existing centrioles in S-phase and reach their full functionality with the formation...

... proposed that the APC/C might fulfil other functions, including assembly of the mitotic spindle. Here, we show that the APC/C localizes to centrosomes, the organizers of the eukaryotic microtubule cytoskeleton, specifically during mitosis. Recruitment of the APC/C to spindle poles requires the centrosomal...

... in ciliary protein trafficking, in the assembly of the T cell synapse. We demonstrated that BBS1 allows for centrosome polarization towards the immune synapse. This function is achieved through the clearance of centrosomal F-actin and its positive regulator WASH1 (also known as WASHC1), a process that we...

... function. Centrosome Cilia Cytoskeleton Mechanobiology Organelle assembly Rootlets Medical Research Council http://dx.doi.org/10.13039/501100007155 University of Cambridge http://dx.doi.org/10.13039/501100000735 Isaac Newton Trust http://dx.doi.org/10.13039...

...Jeffrey C. Medley; Joseph R. DiPanni; Luke Schira; Blake M. Shaffou; Brandon M. Sebou; Mi Hye Song ABSTRACT Aberrant centrosome numbers are associated with human cancers. The levels of centrosome regulators positively correlate with centrosome number. Thus, tight control of centrosome protein...