... and removal of SUMO on substrate proteins. Here, we show that in the fission yeast Schizosaccharomyces pombe , delocalisation of the SUMO protease Ulp1 from the nuclear envelope results in centromeric defects that can be attributed to hyper-SUMOylation at the nuclear periphery. Unexpectedly, we find...

... that SMN relocated to damaged interphase centromeres, together with the Cajal-body-associated proteins coilin and fibrillarin, during the so-called interphase centromere damage response (iCDR). Here we reveal that SMN is a chromatin-binding protein that specifically interacts with methylated histone H3K79...

... mesenchymal stem cells, and quantitatively followed the early changes in nuclear organization during apoptosis. We found that caspase-8 induces alteration of the nuclear lamina and a subsequent spatial reorganization of both centromeres, which are shifted towards a peripheral localization, and telomeres...

...Susan J. Armstrong; F. Christopher H. Franklin; Gareth H. Jones The intranuclear arrangements of centromeres and telomeres during meiotic interphase and early prophase I of meiosis in Arabidopsis thaliana were analysed by fluorescent in situ hybridisation to spread pollen mother cells and embryo...

... parallels that of the MPM2 phosphoepitopes, since it is found in the centrosome and centromere from early prophase until late anaphase, the microtubule-overlapping region during anaphase, and the region on either side of the midbody during telophase. Centromere localisation is not dependent upon...

..., and affinity-purified antibodies from this doublet repro-duced the fluorescence staining pattern of the whole serum. We propose that p330 d is a novel member of the class of ‘chromosomal passenger’ proteins, which are associated transiently with centromeres during early mitosis and are then redistributed...

...Marty F. Bartholdi ABSTRACT The distribution of centromeres in the interphase nuclei of human diploid fibroblasts was analyzed using anti-centromere immunofluorescence and laser scanning confocal microscopy. The positions of the centromeres were placed within the nuclear chromatin distribution...

...Nathalie Chaly; David L. Brown ABSTRACT We have examined early mitotic stages in HeLa cells, mouse 3T3 fibroblasts and mitogen-activated mouse lymphocytes by immunofluorescence labelling with anti-tubulin and anti-centromere. Chromatin organization was monitored with the DNA-specific fluorochrome...