... pseudopodia during phagocytosis. This is consistent with a role for intact ezrin in maintaining folded membrane on the cell surface, which then becomes available for cell spreading and phagocytosis. * Author for correspondence ( [email protected] ) Competing interests The authors declare...

... that following detachment and replating, CLCa is uniquely responsible for promoting efficient cell spreading and migration. Selective depletion of CLCa, but not of CLCb, reduced the initial phase of isotropic spreading of HeLa, H1299 and HEK293 cells by 60–80% compared to siRNA controls, and wound closure...

..., no new membrane is added into the plasma membrane. Pharmacological inhibition of calpain-activation, which is triggered by Ca 2+ influx during neutrophil spreading, prevents normal cell flattening. In calpain-suppressed cells, an aberrant form of cell spreading can occur where an uncoordinated...

... in CD9 recoveries disappeared ( Fig. 6A , right panels). These results suggest that mutant CD9 was not simply left behind in the cell pellet, but rather was reorganized to become partially unavailable (i.e. less soluble) within the Brij 96 lysate. Fig. 3. CD9 mutation affects cell spreading...

...-signal transduction, we performed a small-scale siRNA screen in A549 lung epithelial cells. Of the Rap effectors tested, only Radil blocked Rap-induced spreading. Additionally, we identified a novel role for Ezrin downstream of Rap1. Ezrin was necessary for Rap-induced cell spreading, but not Rap-induced...

... interaction by overexpressing the minimal kinectin–kinesin interaction domain on kinectin in cells. This perturbation resulted in a morphological change to a rounded cell shape and reduced cell spreading and migration. Immunofluorescence and live-cell imaging demonstrated a kinectin-dependent ER extension...

... irrespective of the nature of adhesive contacts. When NMII activity is inhibited during cell spreading by Rho kinase inhibition, blebbistatin, caldesmon overexpression or NMIIA RNAi, the symmetric traction forces are lost and cell spreading persists, causing cytoplasm fragmentation by membrane tension...

... in the regulation of cell spreading and directional migration in response to various stimuli. Here we show that discoidin domain receptor 1 (DDR1), a tyrosine kinase receptor activated by type I collagen, associates with the non-muscle myosin IIA heavy chain (NMHC-IIA) upon ligand stimulation. An association...

... PxxP motifs, which bind Src homology 3 (SH3) domains, are indispensable for the coordinated regulation of filopodium and focal adhesion formation and cell-spreading dynamics during attachment. Candidate Abl PxxP-motif-binding partners were identified by screening a comprehensive SH3-domain phage...

..., impaired cell-cell adhesion, enhanced cell spreading and an increase of β1 integrin activity–effects similar to those of constitutively active R-Ras(38V). Overexpression of ORP3 leads to formation of polarized cell-surface protrusions, impaired cell spreading and decreased β1 integrin activity. In primary...

... fibers Focal adhesions Cell spreading The ability of most cells to survive, proliferate and migrate is regulated in part by the adhesive interactions they make with different components of the surrounding extracellular matrix (ECM), such as fibronectin (FN) ( Boudreau and Jones, 1999...

... to the subfragment 2 domains of nonmuscle myosin IIA and myosin IIB and to the N-terminus of the long form of myosin light chain kinase (L-MLCK). SV inhibits cell spreading via an MLCK- and myosin II-dependent mechanism. Overexpression of SV reduces the rate of cell spreading, and RNAi-mediated knockdown...

...Marko Jović; Naava Naslavsky; Debora Rapaport; Mia Horowitz; Steve Caplan β1 integrins bind to the extracellular matrix and stimulate signaling pathways leading to crucial cellular functions, including proliferation, apoptosis, cell spreading and migration. Consequently, control of β1 integrin...

... Reggie Flotillin Tyrosine phosphorylation Rafts Cell spreading Endocytosis Signaling through growth factor receptors such as the epidermal growth factor receptor (EGFR) involves complex molecular networks of proteins and multiple phosphorylation events, including the autophosphorylation...

...Coert Margadant; Angelique van Opstal; Johannes Boonstra Prevention of cell spreading or disruption of actin filaments inhibits growth factor stimulated cell cycle re-entry from quiescence, mainly because of a failure to induce cyclin D expression. Ectopic cyclin D expression overrules anchorage...

...Aude S. Ada-Nguema; Harry Xenias; Michael P. Sheetz; Patricia J. Keely R-Ras, an atypical member of the Ras subfamily of small GTPases, enhances integrin-mediated adhesion and signaling through a poorly understood mechanism. Dynamic analysis of cell spreading by total internal reflection...

...Katarzyna Bialkowska; Takaomi C. Saido; Joan E. B. Fox In this study, we used cultured cells spreading on β3 integrin substrates to examine the possibility that spectrin is involved in signal transduction. Spectrin clustered with specialized calpain-induced β3 integrin signaling complexes...

...-radixin-moesin (ERM proteins) in the marginal band of chicken erythocytes. J. Cell Sci. 107 , 2523 -2534. Yu, D. H., Qu, C. K., Henegariu, O., Lu, X. and Feng, G. S. ( 1998 ). Protein tyrosine phosphatase Shp-2 regulates cell spreading, migration, and focal adhesion. J. Biol. Chem. 273...

..., King's College London, London SE1 1UL, UK ‡ These authors contributed equally to the work 10 11 2003 © The Company of Biologists Limited 2004 2004 Rac1 Cell spreading GTPase Macrophage The Rho family of small GTPases includes Rac, Rho and Cdc42 proteins, which act...

... actin polymerization that drives extension of the leading cell edge. Subsequently, RhoA regulates myosin-dependent contractile force,which is required for formation of adhesive contacts and stress fibers. During cell spreading, however, the activity of RhoA is reduced by a mechanism involving...