...Paolo Guerra; Luc-Alban P. E. Vuillemenot; Yulan B. van Oppen; Marije Been; Andreas Milias-Argeitis ABSTRACT Recent studies have revealed that the growth rate of budding yeast and mammalian cells varies during the cell cycle. By linking a multitude of signals to cell growth, the highly conserved...

...: Mutations of polycystins lead to the genetic disorder ADPKD. We show that the fission yeast polycystin homolog Pkd2p promotes cell expansion during interphase growth and antagonizes the SIN pathway during cytokinesis. Cell growth Cytokinesis Fission yeast Pkd2 Polycystin SIN pathway Turgor...

...)-containing SCF ubiquitin ligase complex in an auxin-dependent manner, and then the ubiquitylated target proteins are rapidly degraded by proteasomes. We evaluated the Rab1 -CKO and Rab5 -CKO cells in relation to cell survival, cell growth, and organelle distribution and size at various time points after...

...Julie Rich-Robinson; Afton Russell; Eleanor Mancini; Maitreyi Das ABSTRACT In fission yeast, polarized cell growth stops during division and resumes after cytokinesis completes and cells separate. It is unclear how growth reactivation is timed to occur immediately after cell separation. We...

... on the processes related to cell growth and inflammatory response. Our findings reveal the importance of VPS37 paralogs in orchestrating cell homeostasis through maintaining the stability of ESCRT-I. CRC is a leading cause of cancer-associated deaths worldwide because it is often diagnosed in advanced...

.... This work provides standardized methods and resources to decipher the complex mechanisms that control cell growth. This article has an associated First Person interview with Sarah Taheraly, joint first author of the paper. * Present address: Institut Curie, PSL Research University, INSERM U932, 75005...

...Kendall J. Condon; David M. Sabatini ABSTRACT The mechanistic target of rapamycin (mTOR) signaling pathway coordinates environmental and intracellular cues to control eukaryotic cell growth. As a pivot point between anabolic and catabolic processes, mTOR complex 1 (mTORC1) signaling has established...

...-dependent transcription at G1/S, which prevents DNA replication stress. Cell growth Cell cycle Nitrogen Rum1 Ste9 MBF Schizosaccharomyces pombe Ministerio de Economía y Competitividad BFU2011-28274 BFU2014-55439-R and BFU2017-88335-R Junta de Castilla y León CSI084U16...

.... To identify regulators of growth that can reverse this growth arrest, we introduced an ORFeome plasmid library into the pat1 ts mei4Δ strain. Overexpression of eight genes promoted cell growth; two of these were core RNA polymerase subunits, and one was sck2 + , an S6 kinase thought to contribute to TORC1...

... pneumophila . Disrupting both genes additionally leads to defects in development, with strong delay in cell aggregation, formation of large streams and multi-tipped aggregates. Single and double mutants display differential sensitivity to cell growth under conditions of iron overload or depletion. The data...

...Thomas P. Neufeld As a response to nutrient deprivation and other cell stresses, autophagy is often induced in the context of reduced or arrested cell growth. A plethora of signaling molecules and pathways have been shown to have opposing effects on cell growth and autophagy, and results of recent...

... . Fission yeast Gtr1 and Gtr2 are non-essential proteins that enhance cell growth in the presence of amino acids in the medium. The function of Gtr1 and Gtr2 in nutrient signaling is further supported by the observation that even in rich medium the deletion of either gene results in the promotion of mating...

... cytoskeleton Cell growth Development Exon trap Fig. 1. Targeted disruption of αII-spectrin. ( A ) An ES cell line was established using gene trap vector RRQ171 (β-geo) from Bay Genomics. Analysis by 5′ RACE identified insertion of the vector between exons 24 and 25 of the murine Spna2 gene...

...-independent mechanism that links cell growth to cell proliferation in mammalian cells, and suggested that selective targeting of the RNA polymerase I transcription machinery might be advisable to hinder proliferation of p53-deficient cancer cells. Because these results showed that POLR1A interference...

... networks participates in the adaptation of the entire cell polarity with respect to external constraints. These modifications further impact cell migration, growth and differentiation. [email protected] © 2010. 2010 Cell architecture Cell differentiation Cell growth Cell polarity...

... available kit (Life Technologies). The RACE product (560 bp) was sequenced and then ligated to the 2.1-kb clone to generate the near-full-length cDNA (~2.7 kb). Cell growth was measured using a colorimetric assay ( Skehan et al., 1990 ). Briefly, cells (2000 cells/well) were treated with human IFN-β...

...Jian-Bin Wang; Robert Sonn; Yemmsrach K. Tekletsadik; Daniel Samorodnitsky; Mahasin A. Osman Cell proliferation requires close coordination of cell growth and division to ensure constant cell size through the division cycles. IQGAP1, an effector of CDC42 GTPase has been implicated in the modulation...

... Annealing ) used in the PCR cycling conditions in °C is also indicated. * Author for correspondence (e-mail: [email protected] ) 29 11 2007 © The Company of Biologists Limited 2008 2008 DNA replication Ku Cell growth Nascent DNA Replication stress checkpoint...

...Beatriz Álvarez; Sergio Moreno The fission yeast Schizosaccharomyces pombe is an excellent model system in which to study the coordination of cell growth and cell differentiation. In the presence of nutrients, fission yeast cells grow and divide; in the absence of nutrients, they stop growing...

...Stefania Castagnetti; Ralf Behrens; Paul Nurse The rod-shaped Schizosaccharomyces pombe cell grows in a polarized fashion from opposing ends. Correct positioning of the growth zones is directed by the polarity marker Tea1 located at the cell ends where actin patches accumulate and cell growth takes...