... by cholesterol. We prepared reconstituted membranes based on the lipid composition of caveolae. The reconstituted membrane with cholesterol had a weaker affinity for the F-BAR domain of PACSIN2 than a membrane without cholesterol. Consistent with this, upon depletion of cholesterol from the plasma membrane...

...Albert Pol; Frederic Morales-Paytuví; Marta Bosch; Robert G. Parton ABSTRACT Caveolae are invaginations of the plasma membrane that are remarkably abundant in adipocytes, endothelial cells and muscle. Caveolae provide cells with resources for mechanoprotection, can undergo fission from the plasma...

... components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G...

...Alexander Ludwig; Benjamin James Nichols; Sara Sandin ABSTRACT Caveolae are specialized membrane domains that are crucial for the correct function of endothelial cells, adipocytes and muscle cells. Caveolins and cavins are both required for caveolae formation, and assemble into a large (80S...

...Yosuke Senju; Eva Rosenbaum; Claudio Shah; Sayaka Hamada-Nakahara; Yuzuru Itoh; Kimiko Yamamoto; Kyoko Hanawa-Suetsugu; Oliver Daumke; Shiro Suetsugu ABSTRACT PACSIN2, a membrane-sculpting BAR domain protein, localizes to caveolae. Here, we found that protein kinase C (PKC) phosphorylates PACSIN2...

...Asier Echarri; Miguel A. Del Pozo ABSTRACT An essential property of the plasma membrane of mammalian cells is its plasticity, which is required for sensing and transmitting of signals, and for accommodating the tensional changes imposed by its environment or its own biomechanics. Caveolae...

...Oleksiy Kovtun; Vikas A. Tillu; Nicholas Ariotti; Robert G. Parton; Brett M. Collins ABSTRACT Caveolae are an abundant feature of the plasma membrane in many cells. Until recently, they were generally considered to be membrane invaginations whose formation primarily driven by integral membrane...

...Jagan Mohan; Björn Morén; Elin Larsson; Mikkel R. Holst; Richard Lundmark ABSTRACT Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis owing to their dynamic behaviour ranging from stable surface association to dynamic...

.... , Le Guennec   J. Y. , Roger   S. , Gore   J. ( 2011 ). Na(V)1.5 enhances breast cancer cell invasiveness by increasing NHE1-dependent H(+) efflux in caveolae.   Oncogene   30 , 2070 – 2076 . 10.1038/onc.2010.574 Brisson   L. , Reshkin   S. J. , Goré   J. , Roger   S...

.... * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) 22 12 2012 © 2013. Published by The Company of Biologists Ltd 2013 Endothelial cell Shear stress Caveolae Plasma membrane Lipid order Membrane fluidity Giant unilamellar vesicle...

...Othon L. Gervásio; William D. Phillips; Louise Cole; David G. Allen Caveolae are invaginations of the plasma membrane that are formed by caveolins. Caveolar membranes are also enriched in cholesterol, glycosphingolipids and signaling enzymes such as Src kinase. Here we investigate the effect...

... of plasma membrane cholesterol with methyl-β-cyclodexrin, indicating involvement of caveolae in localized ATP release. Ca 2+ imaging with Fluo-4 combined with ATP imaging revealed that shear stress evoked an increase in intracellular Ca 2+ concentration and the subsequent Ca 2+ wave that originated from...

.... Here, we initially confirm that EHD2, a protein previously shown biochemically to be present in caveolar fractions and to bind to pacsins, is a caveolar protein. We go on to report that GFP–pacsin 2 can be recruited to caveolae, and that endogenous pacsin 2 partially colocalises with caveolin 1...

...Emmanuel Boucrot; Mark T. Howes; Tomas Kirchhausen; Robert G. Parton Caveolae form a specialized platform within the plasma membrane that is crucial for an array of important biological functions, ranging from signaling to endocytosis. Using total internal reflection fluorescence (TIRF) and 3D fast...

...Yosuke Senju; Yuzuru Itoh; Kazunori Takano; Sayaka Hamada; Shiro Suetsugu Caveolae are flask-shaped invaginations of the plasma membrane that are associated with tumor formation, pathogen entry and muscular dystrophy, through the regulation of lipids, signal transduction and endocytosis. Caveolae...

... matrix Endocytosis Caveolae Fibrosis Degradation and removal of ECM proteins is a cell-mediated process, which is involved in a number of physiological processes, such as development, postnatal tissue remodeling and tissue repair ( Clark, 1996 ; Holmbeck et al., 1999 ; Vu et al., 1998...

...Matthew Kirkham; Susan J. Nixon; Mark T. Howes; Laurent Abi-Rached; Diane E. Wakeham; Michael Hanzal-Bayer; Charles Ferguson; Michelle M. Hill; Manuel Fernandez-Rojo; Deborah A. Brown; John F. Hancock; Frances M. Brodsky; Robert G. Parton Caveolae are an abundant feature of mammalian cells...

...Susan J. Nixon; Adrian Carter; Jeremy Wegner; Charles Ferguson; Matthias Floetenmeyer; Jamie Riches; Brian Key; Monte Westerfield; Robert G. Parton Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role...

.... The cholesterol-enriched membrane microdomains, caveolae, regulate PDGF receptor signalling in fibroblasts and we examined their role in SFK functions. Here we show that caveolae disruption by membrane cholesterol depletion or expression of the dominant-negative caveolin-3 DGV mutant impaired Src mitogenic...

...Meri M. Vihanto; Cecile Vindis; Valentin Djonov; Douglas P. Cerretti; Uyen Huynh-Do Eph receptor tyrosine kinases are key players during the development of the embryonic vasculature; however, their role and regulation in adult angiogenesis remain to be defined. Caveolae are flask-shaped...