... increase in cellular cAMP and consequent responses in the cytoplasm required for cell–cell fusion. Here, we identify a new participant in ciliary signaling, Gamete-Specific Protein Kinase (GSPK). GSPK is essential for the adhesion-induced cAMP increase and for rapid gamete fusion. The protein...

..., associated with an increase in intracellular cAMP levels. Real-time analysis of cAMP dynamics revealed that PI3Kγ modulates the degradation of cAMP in primary VSMCs independently of its kinase activity through regulation of the enzyme phosphodiesterase 4. Importantly, the use of an N-terminal competing...

...Gergő Szanda; Éva Wisniewski; Anikó Rajki; András Spät ABSTRACT We have previously demonstrated in H295R adrenocortical cells that the Ca 2+ -dependent production of mitochondrial cAMP (mt-cAMP) by the matrix soluble adenylyl cyclase (sAC; encoded by ADCY10 ) is associated with enhanced aldosterone...

...Miguel J. Lobo; Margarida D. Amaral; Manuela Zaccolo; Carlos M. Farinha ABSTRACT Cyclic AMP (cAMP) activates protein kinase A (PKA) but also the guanine nucleotide exchange factor ‘exchange protein directly activated by cAMP’ (EPAC1; also known as RAPGEF3). Although phosphorylation by PKA is known...

... the cellular protein homeostasis. Here, we show that the phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate (PIP 2 ) tethers the F508del CFTR to the actin cytoskeleton, stabilizing it on the apical membrane and rescuing the sub-membrane compartmentalization of cAMP...

...Matthias Gerhardt; Michael Walz; Carsten Beta ABSTRACT Recent work has demonstrated that the receptor-mediated signaling system in chemotactic amoeboid cells shows typical properties of an excitable system. Here, we delivered spatially confined stimuli of the chemoattractant cAMP to the membrane...

... specific AKAPs are depleted by siRNA-mediated knockdown, point to ezrin as a scaffold required for hCG-, cAMP- and PKA-mediated regulation of the fusion process. By a variety of immunoprecipitation and immunolocalization experiments, we show that ezrin directs PKA to a molecular complex of connexin 43...

...Delphine Mika; Wito Richter; Ruth E. Westenbroek; William A. Catterall; Marco Conti ABSTRACT Multiple cAMP phosphodiesterase (PDE) isoforms play divergent roles in cardiac homeostasis but the molecular basis for their non-redundant function remains poorly understood. Here, we report a novel role...

... Dictyostelium , secreted cAMP serves as a chemoattractant, with extracellular cAMP propagated as oscillating waves to ensure directional migratory signals. cAMP oscillations derive from transient excitatory responses of adenylyl cyclase, which then rapidly adapts. We have studied chemotactic signaling...

... XEN cells cultured with GSK3 inhibitor formed adhesion and tight junctions, and acquired bottom-top polarity, indicating the formation of VE cells. In contrast, forskolin, an activator of the cAMP pathway, induced the disruption of cell contacts in rat XEN cells. Treatment with forskolin induced PE...

..., suggesting that the intracellular milieu might affect its ability to respond to cAMP regulation. We recently reported that overexpression of the Na + /H + exchanger regulatory factor NHERF1 in the cystic fibrosis (CF) airway cell line CFBE41o-rescues the functional expression of ΔF508CFTR by promoting F...

...Laura J. Ayling; Stephen J. Briddon; Michelle L. Halls; Gerald R. V. Hammond; Luis Vaca; Jonathan Pacheco; Stephen J. Hill; Dermot M. F. Cooper The central and pervasive influence of cAMP on cellular functions underscores the value of stringent control of the organization of adenylyl cyclases (ACs...

... that cAMP-specific phosphodiesterases (PDE4s) directly bind PAFAH1B1 (also known as LIS1). Dissociation of LIS1–dynein complexes is coupled with loss of dynein function, as determined in assays of both microtubule transport and directed cell migration in wounded monolayers. Such loss in dynein functioning...

...Debbie Willoughby; Sebastian Wachten; Nanako Masada; Dermot M. F. Cooper Ca 2+ -sensitive adenylyl cyclases (ACs) orchestrate dynamic interplay between Ca 2+ and cAMP that is a crucial feature of cellular homeostasis. Significantly, these ACs are highly selective for capacitative Ca 2+ entry (CCE...

... that is relayed to neighboring cells during chemotaxis. Although significant knowledge about cAMP production in chemotaxing cells has been derived from studies performed on cell populations, cAMP dynamics at the single cell level have not been investigated. To examine this, we used a FRET-based cAMP sensor...

...Andreas Schmid; Ge Bai; Nathalie Schmid; Manuela Zaccolo; Lawrence E. Ostrowski; Gregory E. Conner; Nevis Fregien; Matthias Salathe Airway ciliary beat frequency regulation is complex but in part influenced by cyclic adenosine monophosphate (cAMP)-mediated changes in cAMP-dependent kinase activity...

... phagosomal membrane actin assembly provides tracks for lysosomes to move towards phagosomes, thereby facilitating fusion. Here, we investigated how cAMP affected phagosomal actin assembly in vitro, and phagosomal actin, acidification and late fusion events in J774 macrophages. Latex bead phagosomes are shown...

... cyclic AMP (cAMP). A number of kinetic models (including our own) predict that interdependent Ca 2+ and cAMP oscillations can be generated. A previous study in Xenopus neurons illustrated prolonged, low-frequency cAMP oscillations during bursts of Ca 2+ transients. However, the detection of more dynamic...

... Src cAMP Motility Skin The healing of cutaneous wounds is a dynamic, well-organized and complex process requiring the orchestration of many different cell types and cellular processes ( Martin, 1997 ). Dermal fibroblasts are actively involved in this process. They migrate to the wound site...

... of Cx36 by glucose. Glucose or forskolin effects on Cx36 expression were not suppressed by the L-type Ca 2+ -channel blocker nifedipine but were fully blunted by the cAMP-dependent protein kinase (PKA) inhibitor H89. A 4 kb fragment of the human Cx36 promoter was identified and sequenced. Reporter-gene...