... to this canonical nuclear import pathway. Here, we report a new unconventional nuclear import mechanism exploited by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). We found that AcMNPV nucleocapsids entered the nucleus of digitonin-permeabilized cells in the absence of exogenous...

... electrondense membrane structures inside the cytoplasm, which were never seen in Sf9 cells infected with normal baculoviruses (Fig. 2b). These structures appeared to consist of thin parallel or concentric lamellae. Fig. 2. Overexpression of chicken occludin in insect Sf9 cells by recombinant baculovirus...

... was expressed in insect ovarian Sf9 cells using the baculovirus expression system. Approximately half of the induced 52 kDa γ-tubulin was recovered in the supernatant after centrifugation of Sf9 cell lysates at 18,000 g for 15 minutes. When the cell super-natant was analyzed by FPLC on a Superdex 200 sizing...

... acid polypeptide with a calculated molecular mass of 109 kDa. The N-terminal 73% of the antigen was 87% identical to the human clone, whereas the remaining 27% of the coding region showed only 48% homology. Insect Sf9 cells infected with baculovirus containing the full-length insert produced 105 and 95...

... at the C-terminus. * Author for correspondence 01 02 1993 22 03 1993 © 1993 by Company of Biologists 1993 mitosis spindle mitotic poles microtubules autoantibody gene cloning leucine zipper NuMA baculovirus transfection Microtubules are ubiquitous organelles...

... and herpesviruses, baculoviruses replicate within the nucleus and bind detergentinsoluble elements of the nuclear matrix during assembly (Wilson and Price, 1988). Electron microscopic studies of baculovirus-infected cells indicate that during nucleocapsid morphogenesis empty capsids appear to form in association...