... adherens junctions to the force-generating actomyosin cytoskeleton. Drosophila Canoe and mammalian Afadin play key roles. One central task for the field is defining mechanisms by which upstream inputs from Ras-family GTPases regulate Canoe/Afadin. They are unusual in sharing two tandem Ras-association (RA...

...Emily D. McParland; T. Amber Butcher; Noah J. Gurley; Ruth I. Johnson; Kevin C. Slep; Mark Peifer ABSTRACT Robust linkage between adherens junctions and the actomyosin cytoskeleton allows cells to change shape and move during morphogenesis without tearing tissues apart. The Drosophila multidomain...

... by The Company of Biologists Ltd 2023 Summary: E-cadherin and Scribble dynamically relocate during division of single cells to orient the mitotic spindle and control placement of the daughter cells. Scribble E-cadherin Mitotic spindle Retraction fibres Adherens junction Australian...

...Harsha Swamy; Angela J. Glading ABSTRACT Krev-interaction trapped protein 1 (KRIT1) is an endothelial scaffold protein that promotes adherens junction (AJ) stability. The precise mechanism by which KRIT1 promotes barrier stabilization is unclear. We tested the ability of a panel of KRIT1 constructs...

... as they move. These stresses are characteristically tensile in nature and have often been inferred to arise when moving cells pull upon the cell–cell adhesions that hold them together. We now report that epithelial tension at adherens junctions between migrating cells also increases due to an increase in RhoA...

... homeostasis. The adherens junction (AJ) connects the actomyosin networks of neighboring cardiomyocytes and is required for proper heart function. Yet little is known about the molecular composition of the cardiomyocyte AJ or how it is organized to function under mechanical load. Here, we define...

...Bhargavi Manda; Hina Mir; Ruchika Gangwar; Avtar S. Meena; Shrunali Amin; Pradeep K. Shukla; Kesha Dalal; Takuya Suzuki; RadhaKrishna Rao ABSTRACT The apical junctional complex (AJC), which includes tight junctions (TJs) and adherens junctions (AJs), determines the epithelial polarity, cell-cell...

...-cadherin has a tightly folded globular structure with multiple domains that contain determinants of homophilic binding. Cadherin Adherens junction Cell adhesion Atomic force microscopy Insect Evolution The main cell–cell adhesion molecules at adherens junctions are members...

...Robert Sroka; Johan Van Lint; Sarah-Fee Katz; Marlon R. Schneider; Alexander Kleger; Stephan Paschke; Thomas Seufferlein; Tim Eiseler ABSTRACT Dynamic regulation of cell–cell adhesion by the coordinated formation and dissolution of E-cadherin-based adherens junctions is crucial for tissue...

.... This interplay between integrins and cadherins can control fibronectin matrix assembly and signaling, and a fine balance between traction forces at focal adhesions and intercellular tension at adherens junctions is crucial for directional collective cell migration. In this Commentary, we discuss two central...

.... However, the list of identified adherens junction proteins is likely to be incomplete, limiting investigation into this essential cell structure. To expand the inventory of potentially relevant proteins, we expressed E-cadherin fused to biotin ligase in MDCK epithelial cells, and identified by mass...

... upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly...

... they are critical to guarantee rapid apical cell constrictions. Contraction of the apical actomyosin filament system occurs independently of Dzy and Rap1, but loss of Dzy results in a delayed establishment of the apical adherens junction (AJ) belt, whereas in the absence of Rap1 only a fragmentary apical AJ belt...

... complexes: adherens junctions, desmosomes and gap junctions. However, recent morphological and molecular data indicate a mixing of adherens junctional and desmosomal components, resulting in a ‘hybrid adhering junction’ or ‘area composita’. The α-catenin family member αT-catenin, part of the N-cadherin...

... assembly pathways in Drosophila S2 cells . Biochem. Biophys. Res. Commun. 265 , 372 - 375 . Georgiou M. , Marinari E. , Burden J. , Baum B. ( 2008 ). Cdc42, Par6, and aPKC regulate Arp2/3-mediated endocytosis to control local adherens junction stability . Curr. Biol. 18...

...Juan Huang; Lynn Huang; Yi-Jiun Chen; Erin Austin; Caitlin E. Devor; Fabrice Roegiers; Yang Hong Adherens junctions (AJs) in epithelial cells are constantly turning over to modulate adhesion properties under various physiological and developmental contexts, but how such AJ dynamics are regulated...

...Nadine Muschalik; Elisabeth Knust Photoreceptor morphogenesis in Drosophila requires remodelling of apico-basal polarity and adherens junctions (AJs), and includes cell shape changes, as well as differentiation and expansion of the apical membrane. The evolutionarily conserved transmembrane protein...

... and cap cells in Drosophila testes and ovary, respectively) that contacts the basal lamina. Adherens junctions (AJs) anchor the stem cell to the support cell in a stromal niche and maintain contact with neighbours in an epithelial niche. ( B-D ) The effects on support and/or stem cells of integrin...

... intensity; AJ, adherens junctions. Cyto., cytoplasm). Values are mean ± s.d. Note that E-cadherin suppression by the ΔC1A, ΔC1B or ΔC2 mutant is associated with fewer organized β-catenin and actin filaments at cell-cell junctions. Fig. 6. The hinge region of PKCδ is required for the targeting of cell-cell...

...Lysianne Follonier; Sébastien Schaub; Jean-Jacques Meister; Boris Hinz Neoformation of intercellular adherens junctions accompanies the differentiation of fibroblasts into contractile myofibroblasts, a key event during development of fibrosis and in wound healing. We have previously shown...