... in any direction without turning. This implies that any part of the chloroplast periphery can function as the leading or trailing edge during movement. This ability is mediated by a special structure, which consists of short actin filaments that are polymerized at the leading edge of moving chloroplasts...

... cell surface expansions. Genes able to mitigate SMN deficiency operate within pathways in which SMN can act, such as mRNA translation, actin network and endocytosis. Here, we found that SMN accumulates at membrane protrusions during the dynamic rearrangement of the actin filaments. In addition...

... of Mug33 translocation on Myo52 strongly suggest that translocation of Mug33 TVEs on actin filaments is driven by Myo52. This is similar to post-Golgi trafficking in budding yeast, which depends on the type V myosin Myo2p ( Govindan et al., 1995 ; Pruyne and Bretscher, 2000 ; Pruyne et al., 1998...

...G. Bradley Alsop; Dahong Zhang We systematically examined the impact of microtubules on distribution of actin filaments and positioning of cell cleavage using micromanipulation to progressively alter the symmetric distribution of spindle microtubules in grasshopper spermatocytes. The initial...

... ). An improved method for determining the actin filament content of nonmuscle cells by the DNAase I inhibition assay. Anal. Biochem. 117 , 170 -177. Franck, P. F., Bevers, E. M., Lubin, B. H., Comfurius, P., Chiu,D. T., Op den Kamp, J. A., Zwaal, R. F., van Deenen, L. L. and Roelofsen,B. ( 1985...

... translocation pathway of S100A13 in ECV, which is unique for the S100 family since S100 proteins lack the classic signal sequence for secretion. Other secretion pathways such as the actin filament pathway, which can be blocked by amlexanox ( Tarantini et al., 2001 ), and the tubulin-dependent translocation...

... 2001 by Company of Biologists 2001 Chloroplast movement Microtubule Actin filament Phytochrome Blue light receptor Intracellular movement of organelles is an indispensable event for many cellular functions. Unlike animals, plants cannot change their location from where they start...

... of peripheral actin filaments. Neither Clostridium botulinum C3 toxin, which selectively ADP-ribosylates Rho, nor Clostridium sordellii lethal toxin, which inactivates Rac, affected cortical actin, suggesting that Cdc42 plays a specific role in the organization of subplasmalemmal actin. Indeed, toxin B strongly...

... at the leading edge and move retrogradely to reach the base of the growth cone within a lapse of 30-60 seconds. Such velocity (5.7 μm/minute) is consistent with a coupling between F3 receptors and the retrograde flow of actin filaments. When actin filaments were disrupted by cytochalasin B, the F3Fc beads...

...Joel S. Tabb; Bradley J. Molyneaux; Darien L. Cohen; Sergei A. Kuznetsov; George M. Langford ABSTRACT Axoplasmic organelles in the giant axon of the squid have been shown to move on both actin filaments and microtubules and to switch between actin filaments and microtubules during fast axonal...

... by Company of Biologists 1998 EGFP-actin Cell migration Cell adhesion Actin filament Actin is a highly conserved and abundant cytoskeletal protein in eukaryotic cells. It is implicated in a number of cellular activities, including reorganization of cell shape and cell motility ( Stossel...

... the subcellular localization of isoforms of G protein γ subunits in Swiss 3T3 and C6 glioma cells, mainly containing the γ 5 and γ 12 subunits. Immunocytochemical double staining with phalloidin showed co-localization of the γ 12 subunit with actin filaments (F-actin), while the γ 5 co-localized with vinculin...

... by rearrangement of the cytoskeleton. To identify changes in the organization of actin filaments and microtubules that occur as growth cones turn, we used time-lapse phase contrast videomicroscopy to observe embryonic chick dorsal root ganglion neuronal growth cones at a substratum border between fibronectin...

... Actin filament ATP-dependence Fig. 1. Light and electron micrographs of tubular epithelial cells of the P 3 -segment of canine nephron incubated for catalase activity. (Inset in A) Peroxisomes are abundant and clustered in the basolateral cytoplasm. (A) Microtubules are frequently seen in close...

... the track of their movement. Fluorescent phallotoxins and monoclonal antiactin antibodies label filament bundles in the submicrovillar region, indicating that they are composed of F-actin. Finally, depolymerization of the submicrovillar actin filaments by incubation with cytochalasin B results in a blockade...

...Shigenobu Yonemura; Masahiko Itoh; Akira Nagafuchi; Shoichiro Tsukita ABSTRACT Cadherin has an intimate spatial relationship with actin filaments (AF) in various types of cells, forming the cell-to-cell adherens junction (AJ). We compared the AJ/AF rela-tionship between non-polarized fibroblasts...

... of the host cell. By recruiting actin filaments into a ‘comet tail’ localized at one pole of the bacterial cell wall, Listeria become mobile and propel themselves through the cytoplasm. They create protrusions at the plasma membrane that can invaginate adjacent cells. In this work, we have analysed...

...George M. Langford; Sergei A. Kuznetsov; Dale Johnson; Darien L. Cohen; Dieter G. Weiss ABSTRACT The directionality of the actin-dependent motors on squid axoplasmic organelles was determined using actin filaments assembled on the barbed ends of acrosomal processes. Acrosomal processes were...

...Issei Mabuchi ABSTRACT Cleavage furrow formation at the first cell division of sea urchin and sand dollar eggs was investigated in detail by fluorescence staining of actin filaments with rhodamine-phalloidin of either whole eggs or isolated egg cortices. Cortical actin filaments were clustered...

... filaments are densely associated with plasma membranes. 31 03 1992 28 05 1992 © 1992 by Company of Biologists 1992 ezrin radixin moesin actin filament In this study, we examined the distribution of the members of the ERM family. Taking both crossreaction and masking problems...