...; Rayah Alsayed; Benjamin J. Perrin; Scott W. Crawley Myosin-7A (Myo7A) is a motor protein crucial for the organization and function of stereocilia, specialized actin-rich protrusions on the surface of inner ear hair cells that mediate hearing. Mutations in Myo7A cause several forms of genetic hearing...

... in common use, aspects of their cellular architecture and mechanobiology remain insufficiently understood. Here, we investigated how the actin cytoskeleton is regulated in different pluripotency states. We observed a drastic reorganization during the transition from ground-state naïve mouse embryonic stem...

... stimuli. Emerging evidence highlights the importance of intrinsic nuclear mechanisms in the mechanoresponse. The discovery of actin and associated motor proteins, such as myosins, in the nucleus, along with advances in chromatin organisation research, has raised new questions about the contribution...

... cilia of murine intramedullary collecting duct cells. The shortening of primary cilia caused by hyperosmotic shock followed delocalization of the pericentriolar material (PCM). Excessive microtubule and F-actin formation in the cytoplasm coincided with the hyperosmotic shock-induced changes to primary...

... organelles, heavily regulated cytoskeletal networks and complex signaling cascades. Because the actin cytoskeleton interfaces with each of these features, understanding how it evolved and diversified across eukaryotic phyla is essential to understanding the evolution and diversification of eukaryotic cells...

... tight junctions, and the mechanisms and regulation of the tight junction barrier in physiology and pathology. Actin Claudin Epithelium Myosin Permeability Tight junctions ZO-1 Cingulin Occludin Barrier Polarity Canton of Geneva http://dx.doi.org/10.13039/501100011723...

... in outer segment coverage between photoreceptor subtypes. CP length is stable across light and dark conditions, yet heat-shock inducible expression of tagged actin revealed rapid turnover of the CP actin core. Detailed imaging of the embryonic retina uncovered substantial remodeling of the developing...

...Thomas C. A. Blake; Helen M. Fox; Vasja Urbančič; Roshan Ravishankar; Adam Wolowczyk; Edward S. Allgeyer; Julia Mason; Gaudenz Danuser; Jennifer L. Gallop ABSTRACT Filopodia are narrow actin-rich protrusions with important roles in neuronal development where membrane-binding adaptor proteins...

...Laura E. McCormick; Cristian Suarez; Laura E. Herring; Kevin S. Cannon; David R. Kovar; Nicholas G. Brown; Stephanie L. Gupton ABSTRACT The actin cytoskeleton performs multiple cellular functions, and as such, actin polymerization must be tightly regulated. We previously demonstrated...

...Thomas A. Phillips; Stefania Marcotti; Susan Cox; Maddy Parsons ABSTRACT The actin cytoskeleton plays a critical role in cell architecture and the control of fundamental processes including cell division, migration and survival. The dynamics and organisation of F-actin have been widely studied...

...Matthieu Gélin; Alexandre Schaeffer; Jérémie Gaillard; Christophe Guérin; Benoit Vianay; Magali Orhant-Prioux; Marcus Braun; Christophe Leterrier; Laurent Blanchoin; Manuel Théry ABSTRACT The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates...

...Sabrina M. Holland; Gianluca Gallo ABSTRACT The regulation of the intracellular level of ATP is a fundamental aspect of bioenergetics. Actin cytoskeletal dynamics have been reported to be an energetic drain in developing neurons and platelets. We addressed the role of actin dynamics in primary...

...) with DAVID of the top 50 proteins from the DTSSP 10G5 eluate. Red, actin-associated components; orange, intermediate filament-associated components; blue, microtubule-associated components; gray, other components. (G) Overlap of protein interaction networks of key proteins from the DTSSP 10G5 eluate (GIPC3...

... and promotes integrin–talin–actomyosin coupling. Actin Integrin Kindlin Membrane Talin Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 ANR-18-CE13-0026-01 ANR-21-CE13-0010-03 ANR-20-CE13-0016 Cell adhesion and migration are controlled by biochemical...

...Michael Bachmann; Baihao Su; Rolle Rahikainen; Vesa P. Hytönen; Jinhua Wu; Bernhard Wehrle-Haller ABSTRACT Talin (herein referring to the talin-1 form), is a cytoskeletal adapter protein that binds integrin receptors and F-actin, and is a key factor in the formation and regulation of integrin...

...Melissa A. Quintanilla; John A. Hammer; Jordan R. Beach ABSTRACT Non-muscle myosin 2 (NM2) motors are the major contractile machines in most cell types. Unsurprisingly, these ubiquitously expressed actin-based motors power a plethora of subcellular, cellular and multicellular processes...

...Franziska Lehne; Sven Bogdan ABSTRACT Exocytosis is a fundamental cellular process by which cells secrete cargos from their apical membrane into the extracellular lumen. Cargo release proceeds in sequential steps that depend on coordinated assembly and organization of an actin cytoskeletal network...

... molecular motors and other proteins are immobilized on artificial surfaces. In this Perspective, I discuss the progress towards practically viable applications achieved so far using the myosin II–actin motor–filament system. I also highlight several fundamental pieces of insights derived from the studies...

...Stephen M. Coscia; Cameron P. Thompson; Qing Tang; Elana E. Baltrusaitis; Joseph A. Rhodenhiser; Omar A. Quintero-Carmona; E. Michael Ostap; Melike Lakadamyali; Erika L. F. Holzbaur ABSTRACT Mitochondrial homeostasis requires a dynamic balance of fission and fusion. The actin cytoskeleton promotes...

..., for intracellular transport. Here we describe how molecular motors and cytoskeletal tracks can generate streaming. Actin Cytoplasmic streaming Dynein Kinesin Microtubules Myosin National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35 GM131752...