.../ Highlighted Article: Phosphorylation and dephosphorylation regulate clustering of PAR3, a cell polarity-regulating factor, and this clustering affects the localization of PAR3 and cell–cell junctions. PAR3 PAR-3 ASPP2 aPKC Epithelium Polarity Ministry of Education, Culture, Sports...

...Indrasen Magre; Vikas Fandade; Indraneel Damle; Poulomi Banerjee; Santosh Kumar Yadav; Mahendra Sonawane; Jomon Joseph ABSTRACT The Par polarity complex, consisting of Par3, Par6 and atypical protein kinase C (aPKC), plays a crucial role in the establishment and maintenance of cell polarity...

... by the unspecific nature of interventions. Here we address this problem in relation to atypical PKC (aPKC) action, which in conjunction with the exocyst complex controls the polarised delivery of promigratory signals. A drug-dependent recruitment approach was employed to manipulate the local recruitment of signals...

...Hironori Ogawa; Nao Ohta; Woongjoon Moon; Fumio Matsuzaki Drosophila neural stem cells or neuroblasts undergo typical asymmetric cell division. An evolutionally conserved protein complex, comprising atypical protein kinase C (aPKC), Bazooka (Par-3) and Par-6, organizes cell polarity to direct...

... to unpolarized neurons. Early stages of neuronal polarization are accompanied by increasing levels of interaction of the exocyst complex with PAR-3 and atypical protein kinase C (aPKC), and by the RalA-dependent association of the exocyst complex with PAR-3. Thus, neuronal polarization involves a RalA-regulated...

...Yosuke Horikoshi; Atsushi Suzuki; Tomoyuki Yamanaka; Kazunori Sasaki; Keiko Mizuno; Hajime Sawada; Shigenobu Yonemura; Shigeo Ohno The evolutionarily conserved polarity proteins PAR-3, atypical protein kinase C (aPKC) and PAR-6 critically regulate the apical membrane development required...

... the truncated Nagie oko protein inactive and recreates all features of the nagie oko mutant phenotype when tested in functional complementation assays. Our observations reveal a previously unknown diversity of alternative multi-protein assembly compositions of the Crumbs–Nagie-oko and Par6-aPKC protein...

...Masaru Kishikawa; Atsushi Suzuki; Shigeo Ohno Atypical protein kinase C (aPKC) generally plays crucial roles in the establishment of cell polarity in various biological contexts. In mammalian epithelial cells, aPKC essentially works towards the transition of primordial spot-like adherens junctions...

... that they are part of an evolutionarily conserved PAR-aPKC system involved in cell polarity in various biological contexts. Recent progress has illustrated the common principle that the PAR-aPKC system is the molecular machinery that converts initial polarity cues in the establishment of complementary membrane...

.../aPKC complex has a role in establishing cellular polarity and division orientation in other systems, we explored its potential function in the developing mouse embryo. We show that both Par3 and aPKC adopt a polarized localization from the 8-cell stage onwards and that manipulating their function re...

...Atsushi Suzuki; Chikako Ishiyama; Katsutaka Hashiba; Miki Shimizu; Klaus Ebnet; Shigeo Ohno We have previously shown that aPKC interacts with cell polarity proteins PAR-3 and PAR-6 and plays an indispensable role in cell polarization in the C. elegans one-cell embryo as well as in mammalian...