... inhibitors have been described including ZM447439, which prevents chromosome alignment, spindle checkpoint function and cytokinesis. Subsequently, ZM447439-treated cells exit mitosis without dividing and lose viability. Because ZM447439 inhibits both Aurora A and B, we set out to determine which phenotypes...

...; MG132, 20 μM; and ZM447439, 2 μM. All cell culture conditions were as described previously (Taylor et al., 2001). To enrich for mitotic cells, cultures were treated overnight with either taxol or nocodazole. For immunoblot analysis, soluble cell proteins or immune complexes were resuspended...